View source for Template:Team:Groningen/CONTENT/EXPERIMENTS/Restriction thrC compleet, tasA, bslA and slrR Inserts (th21)

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	|title=00:00, 6 September 2015 - 00:00, 6 September 2015
	|executor=Marieke Mulder
	|content=
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the following samples were digested.

 
construct
restriction enzymes
ThrC backbone with EcoRI and PstI. 
Salt promoter with TasA in pSB1C3 with EcoRI and PstI. 
Salt promoter with YuaB in pSB1C3 with EcoRI and PstI. 
SlrR in pSB1C3 with EcoRI and PstI.

 
restriction were performed for 2 hours at 37°C in stopped by heating at 80°C for 20 min.


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