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    <div class="jamboreh">


      <h1>June</h1>


    <table align=center width="100%" border=1>
    <tr>
      <td align=middle  width="14.28%" bgcolor=#04A872> Monday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Tuesday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Wednesday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Thursday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Friday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Saturday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Sunday </td>
    </tr> <tr>
     <td valign=top align=left> 01/06
    </td>
     <td valign=top> 02/06
       
         <br> - iGEM kit arrived!
       
     </td>
     <td valign=top align=left> 03/06       
     </td>
     <td valign=top align=left> 04/06
     </td>
     <td valign=top align=left> 05/06
     </td>
     <td valign=top align=left> 06/06
     </td>
     <td valign=top align=left> 07/06
     </td>
    </tr> <tr>
     <td valign=top align=left> 08/06</td>
     <td valign=top align=left>  09/06
       
                           <br>- Transformation:
                              <br><font color=#2cc22c>- B0034
                              <br>- K1497008
                              <br>- R0010
                              <br>- K914003
                              <br>- R0080
                              <br>- C0040
                              <br>- B0015
                              <br>- R0040
                              <br>- E0840
                              <br>- E0040
                              <br>- P0440
                              <br>- I13507</br></font>

     </td>
     <td valign=top align=left> 10/06
     </td>
       
     <td valign=top align=left> 11/06
       
                           <br>- Inoculum
                              <br><font color=#2cc22c>- B0034
                              <br>- K1497008
                              <br>- R0010
                              <br>- K914003
                              <br>- R0080
                              <br>- C0040
                              <br>- B0015
                              <br>- R0040
                              <br>- E0840
                              <br>- E0040
                              <br>- P0440
                              <br>- I13507</br></font>
     </td>
     <td valign=top align=left> 12/06
       
                        <br>- Miniprep and Digestion EXSP
                              <br><font color=#2cc22c>- B0034
                              <br>- K1497008
                              <br>- R0010
                              <br>- K914003
                              <br>- R0080
                              <br>- C0040
                              <br>- B0015
                              <br>- R0040
                              <br>- E0840
                              <br>- E0040
                              <br>- P0440
                              <br>- I13507</br></font>

     </td> 
     <td valign=top align=left> 13/06
       
         <p><br>- Agarose gel (Problems with the buffer! Way too concentrated ><)
         <br>- Digestion EXSP again (<font color=#2cc22c>C0040</font>, <font color=#2cc22c>B0015</font>, <font color=#2cc22c>R0040</font>, <font color=#2cc22c>E0840</font>, <font color=#2cc22c>E0040</font>, <font color=#2cc22c>P0440</font>, <font color=#2cc22c>I13507</font>) [We didn’t have <font color=#2cc22c>B0034</font> anymore, so we didn’t prepare the reaction for <font color=#2cc22c>K1497008</font>, <font color=#2cc22c>R0010</font>, <font color=#2cc22c>K914003</font>, <font color=#2cc22c>R0080</font>]</p>
     </td>
     <td valign=top align=left> 14/06
         <p>
         <br>- Agarose gel(<font color=#2cc22c>C0040</font>, <font color=#2cc22c>B0015</font>, <font color=#2cc22c>R0040</font>, <font color=#2cc22c>E0840</font>, <font color=#2cc22c>E0040</font>, <font color=#2cc22c>P0440</font>, <font color=#2cc22c>I13507</font>)
         <br>- Gel purification (<font color=#2cc22c>C0040</font>, <font color=#2cc22c>B0015</font>, <font color=#2cc22c>E0040</font>, <font color=#2cc22c>P0440</font>)
         <br>- Interlab transformations (<font color=#2cc22c>J23101</font>, <font color=#2cc22c>J23106</font>, <font color=#2cc22c>J23117</font>, <font color=#2cc22c>I13504</font>)
         <br>- Ligation Tet_term (C0040 + B0015)</font>
         <br>- Ligation GFP_P0440 (E0040 + P0440)</font></p>     
     <tr>
     <td valign=top align=left> 15/06
       
         <br>- Transformation Tet_term (C0040 + B0015)
         <br>- Transformation GFP_P0440 (E0040 + P0440)
         <br>- Interlab inoculum
         <br>- Inoculum (B0034)
       
     </td>
     <td valign=top align=left> 16/06
       
         <br>- Inoculum (Tet_term, GFP_P0440)
         <br>- Inoculum (Tet_term, GFP_P0440)
         <br>- Interlab minipreps
         <br>- Miniprep (B0034)
         <br>- Digestion ESXP (B0034, B0034, K1497008, R0010, K914003, R0040, E0840)

     </td>    
   
     <td valign=top align=left> 17/06
       
         <br>- Miniprep (Tet_term, GFP_P0440)
         <br>- Agarose gel (B0034, B0034, K1497008, R0010, K914003, R0040, E0840)
         <br>- Gel purification (B0034, B0034, K1497008, R0010, K914003, R0040, E0840)
         <br>- Ligation RBS_hokD (B0034 + K1497008)
         <br>- Ligation Pl_RBS (R0010 + B0034)
         <br>- Ligation Pt_E0840 (R0040 + E0840)
         <br>- Ligation Pt_I13507 (R0040 + I13507)

       
     </td>
     <td valign=top align=left> 18/06
       
         <br>- Transformation (RBS_hokD, Pl_RBS, Pt_E0840, Pt_I13507)
     </td>
       
     <td valign=top align=left> 19/06
       
         <br>- No colonies! Wrong antibiotic!!!!
         <br>- New transformation (RBS_hokD, Pl_RBS, Pt_E0840, Pt_I13507)

       
     </td>
     <td valign=top align=left> 20/06
       
         <br>-  Inoculum (Pl_RBS, Pt_E0840, Pt_I13507)
         <br>-  RBS_hokD didn’t grow

   
     </td>
       
     <td valign=top align=left> 21/06
       
         <br>-  Miniprep (Pl_RBS, Pt_E0840, Pt_I13507)
         <br>-  Digestion NotI  (Tet_term, GFP_P0440, Pl_RBS, Pt_E0840, Pt_I13507)

       
     </td>
     </tr>     
     <td valign=top align=left> 22/06
       
         <br>- Confirmation, agarose gel (Tet_term, GFP_P0440, Pl_RBS, Pt_E0840, Pt_I13507)
         <br>- Confirmed (Tet_term, Pl_RBS, Pt_E0480)
         <br>- Ligation (GFP_P0040, Pt_I13507, RBS_hokD)

       
     <td valign=top align=left> 23/06
       
         <br>- Transformation (GFP_P0040, Pt_I13507, RBS_hokD)
       
     </td>
     <td valign=top align=left> 24/06
       
         <br>- Inoculum [GFP_P0040 (3 colonies), RBS_hokD (3 colonies), I13507, R0080, K914003]
     </td>
  
     <td valign=top align=left> 25/06
       
         <br>-  Miniprep [GFP_P0040 (3 colonies), RBS_hokD (3 colonies), I13507, R0080, K914003]
       
     </td>
     <td valign=top align=left> 26/06
       
     </td>
       
     <td valign=top align=left> 27/06
       
         <br>- EXSP digestion (K91403)
         <br>- Confirmation digestion (RBS_hokD)

       
     </td>
     <td valign=top align=left> 28/06
       
         <br>- Agarose gel (K91403)
         <br>- Agarose gel (RBS_hokD) – positive result \o/
         <br>- Gel purification (K91403)
         <br>- Ligation Pr_RBS (K91403 + B0034) 22ºC for 4h, 16ºC for 10h, 4º overnight
         <br>- EXSP digestion RBS_hokD
         <br>- Interlab digestion (from minipreps of June 15th)
         <br>- Promoters J23101,J23106 and J23117 - SP enzymes
         <br>- GFP part I13504(RBS B0034+GFP E0040+double Term B0010 and B0012 ) - XP enzymes

       
     </td>
     <tr>
     </tr>     
     <td valign=top align=left> 29/06
       
         <br>- Agarose gel RBS_hokD
         <br>- Ligation (Pr_RBS, Pa_RBS)

       
     </td>
     <td valign=top align=left> 30/06
       
         <br>- Inoculum RBS_hokD
         <br>- EXSP digestion RBS_hokD

       
     </td>
     
     
   </table>
   

       <h1>July</h1>


    <table align=center width="100%" border=1>
    <tr>
      <td align=middle  width="14.28%" bgcolor=#04A872> Monday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Tuesday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Wednesday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Thursday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Friday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Saturday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Sunday </td>
    </tr> <tr>
<td><td></td></td>


     <td valign=top> 01/06

		<br>
                 - Agarose gel (RBS_hokD) <br><br>
                 - Gel purification (RBS_hokD) <br><br>
                 - Ligation 2X_RBS_hokD (RBS_hokD + RBS_hokD) <br><br>
                 - EXSP Digestion (P0040, Pl_RBS, GFP_R0440) <br><br>
                 - Agarose gel (P0040) <br><br>
                 - Gel purification (P0040) <br><br>
                 - Ligation Pt_I13507(P0040 + I13507) <br><br> 
                 - Interlab gel purification and ligation <br><br>
                 <br>
     <td valign=top> 02/06
       
<br>        	
- Transformation Ptet_I13507<br><br>
- Agarose gel (Pl_RBS, GFP_P0440)<br><br>
- Gel purification (Pl_RBS, GFP_P0440)<br><br>
- Ligation Pl_P0440 (Pl_RBS + GFP_P0440)<br><br>
- Interlab transformation (with wrong antibiotic ampicillin - is possible found in registry the same parts in another backbone BBa_J61002)<br><br>
<br>
       
     </td>
     <td valign=top align=left> 03/06

<br>       
- Transformation (Pr_RBS, 2X_RBS_hokD)<br><br>
- EXSP digestion (P0440, R0040)<br><br>
- Transformation Pl_R0440 (Pl_RBS + GFP_P0440)<br><br>
- Inoculum (Pt_I13507)<br><br>
<br>
       
     </td>
     <td valign=top align=left> 04/06
<br>
- Agarose gel (P0440, R0040)<br><br>
- Gel purification (P0440, R0040)<br><br>
- Ligation tet_Ptet (P0440 + R0040)<br><br>
- Inoculum (2X_RBS_hokD)<br><br>
- Miniprep (Pt_I13507)<br><br>
<br>
     </td>
     <td valign=top align=left> 05/06
<br>
- Inoculum (R0080, K91403)<br><br>
- EXSP digestion (tet_Term, Pt_E0840)<br><br>
- Miniprep (2X_RBS_hokD)<br><br>
<br>


     </td>
 
<tr>
     <td valign=top align=left> 06/06
<br>     
- NotI digestion (2X_RBS_hokD, Pr_RBS, Pa_RBS)<br><br>
- Transformation (tet_Ptet)<br><br>
- Agarose gel (2X_RBS_hokD) – positive results<br><br>
- Agarose gel (tet_Term, Pt_E0840)<br><br>
- EXSP digestion (Pt_E0480)<br><br>
- Inoculum (Pl_P0440)<br><br>
- NotI digestion (Pt_I13507) – positive results <br><br>
<br>

    </td>
     <td valign=top align=left> 07/06
<br>
- Agarose gel (Pr_RBS, Pa_RBS, Pt_E0480) – positive results for Pr_RBS and Pt_E0840)<br><br>
- EXSP digestion (2X_RBS_hokD, B0015)<br><br>
- Ligation Term_E0840 (Pt_E0840 + tet_Term)<br><br>
- Inoculum (tet_Ptet)<br><br>
- Transformation (Term_E0840)<br><br>
- Miniprep (Pl_P0440)<br><br>
- Not digestion (Pl_P0440) – positive results<br><br>
- EXSP digestion (Pl_P0440, Pt_I13507)<br><br>
- Inoculum (GFP_P0440)<br><br>
<br>
     </td>
     
    <td valign=top align=left> 08/06
<br>
- Agarose gel (2X_RBS_hokD, B0015) – B0015 didn’t digest<br><br>
- Gel purification (2X_RBS_hokD)<br><br>
- EXSP digestion (B0015)<br><br>
- Miniprep (tet_Ptet)<br><br>
- NotI digestion (tet_Ptet)<br><br>
- Agarose gel (tet_Ptet, B0015) – positive results<br><br>
- Gel digestion (B0015)<br><br>
- Ligation hokD_Term (2X_RBS_hokD + B0015)<br><br>
- Ligation Pl_I13507 (Pl_P0440 + Pt_I13507)<br><br>
- Miniprep (GFP_P0440)<br><br>
- EXSP digestion (GFP_P0440)<br><br>
<br>
 </td>
     <td > 09/06
<br>       
- Agarose gel and gel purification (GFP_P0440)<br><br>
- Ligation GFP_I13507 (GFP_P0440 + Pt_I13507)<br><br>
- Transformation (Pl_I13507)<br><br>
<br>
     </td>
     <td valign=top align=left> 10/06
<br>       
- Transformation (hokD_Term)<br><br>
- Inoculum (Pl_I13507)<br><br>
- Transformation (GFP_I13507)<br><br>
<br>

     <td valign=top align=left> 11/06
<br>       
- Miniprep (Pl_I13507)<br><br>
<br> 
     </td>

     <td valign=top align=left> 12/06
       
</tr>
<tr>
       
     <td valign=top align=left> 13/06
<br>       
- Inoculum (GFP_I13507)<br><br>
- NotI digestion (Pl_I13507)<br><br>
- Agarose gel (Pl_I13507) – negative results<br><br>
<br>
     </td>
    
 <td valign=top align=left> 14/06

<br>       
- Miniprep (GFP_I13507)<br><br>
- NotI digestion (GFP_I13507) – negative results<br><br>
- EXSP digestion (Pl_P0440, Pt_I13507)<br><br>
- Inoculum (GFP_P0440, Pt_I13507)<br><br>
<br>
     
     
     <td valign=top align=left> 15/06
<br>       
- Agarose gel (Pl_P0440, Pt_I13507)<br><br>
- Gel purification (Pl_P0440, Pt_I13507)<br><br>
- Ligation Pl_I13507 (Pl_P0440 + Pt_I13507)<br><br>
- Miniprep (GFP_P0440, Pt_I13507)<br><br>
- EXSP digestion (GFP_P0440, Pt_I13507)<br><br>
<br>

       
     </td>
     <td valign=top align=left> 16/06
       
<br>
- Agarose gel and gel purification (GFP_P0440, Pt_I13507)<br><br>
- Ligation GFP_I13507 (GFP_P0440 + Pt_I13507)<br><br>
- Transformation (Pl_I13507)<br><br>
<br>

       
     <td valign=top align=left> 17/06
<br>       
- Transformation (GFP_I13507)<br><br>
- Inoculum (Pt_Term, Pl_I1350)<br><br>
<br>       


     </td>
     <td valign=top align=left> 18/06
<br>       
- Miniprep (Pt_Term, Pl_I13507)<br><br>
- NotI digestion (Pt_Term, Pl_I13507)<br><br>
- Agarose gel (Pt_Term, Pl_I13507) – positive results for Pt_Term<br><br>
<br>       
     <td valign=top align=left> 19/06

     </tr>
<tr>
  
     </td>
     <td valign=top align=left> 20/06
       
         <li><font size=2> iGEM kit arrived!</font></li>
       
     <td valign=top align=left> 21/06
<br>       
- Inoculum (GFP_I13507, Pl_I13507)<br><br>
- New interlab transformations<br><br>
<br>
     </td>
     
     <td valign=top align=left> 22/06
<br>       
- Miniprep (GFP_I13507, Pl_I13507)<br><br>
- NotI digestion (GFP_I13507, Pl_I13507)<br><br>
- EXSP digestion (Pr_RBS, R0080)<br><br>
- Agarose gel (GFP_I13507, Pl_I13507, Pr_RBS)<br><br>
- Gel purification (Pr_RBS)<br><br>
- Inoculum (GFP_I13507, Pl_I13507)<br><br>
- Interlab inocula<br><br>
<br>
     <td valign=top align=left> 23/06
<br>       
- Agarose gel (R0080)<br><br>
- Miniprep (GFP_I13507, Pl_I13507)<br><br>
- NotI digestion (GFP_I13507, Pl_I13507)<br><br>
- Agarose gel (GFP_I13507, Pl_I13507) – Positive results for GFP_I13507<br><br>
- EXSP digestion (GFP_I13507)<br><br>
- Interlab miniprep and restriction analysis<br><br>
<br>

       
     </td>
     <td valign=top align=left> 24/06
<br>       
- Miniprep (B0015, Pt_P0440)<br><br>
- EXSP digestion (2X_RBS_hokD, B0015)<br><br>
- Transformation (Pa_RBS)<br><br>
- Agarose gel of restriction analysis: devices confirmed<br><br>
<br>

       
     <td valign=top align=left> 25/06

<br>       
- agarose gel (2X_RBS_hokD, B0015) - B0015 did not digest<br><br>
<br>
       
     </td>
     <td valign=top align=left> 26/06
<br>       
- Agarose gel and gel purification (GFP_I13507)<br><br>
- Ligation Pr_I13507 (Pr_RBS + GFP_I13507)<br><br>
<br>
     </tr>
     <tr>


     <td valign=top align=left> 27/06
<br>       
- EXSP digestion (Pl_RBS, Pt_Term)<br><br>
- Transformation (Pr_I13507, Pa_RBS)<br><br>
<br>
       
     </td>
     <td valign=top align=left> 28/06
<br>       
- Agarose gel (Pl_RBS, Pt_Term)<br><br>
ligation Pr_I13507(Pr_RBS + Pt_Term)<br><br>
<br>
     </td>
          
     <td valign=top align=left> 29/06
       
<br>        
- EXSP digestion (B0015, 2X_RBS_hokD)<br><br>
- agarose gel (B0015, 2X_RBS_hokD) - 2X_RBS_hokD didn’t digest<br><br>
- EXSP digestion overnight (2X_RBS_hokD)<br><br>
- transformation (Pr_Term, Pl_RBS)<br><br>
<br>
     </td>
     <td valign=top align=left> 30/06
<br>       
- inoculum (Pl_RBS, Pa_RBS)<br><br>
- agarose gel (B0015, 2X_RBS_hokD) - Digestion worked!<br><br>
- ligation 2X_hokD_Term (B0015 + 2X_RBS_hokD) P0440_hokD(P0440_term + 2X_RBS_hokD)<br><br>
<br>

       
     </td>
     <td valign=top align=left> 31/06
       
        
       
     </td>
     
   </table>


<h1>August</h1>


    <table align=center width="100%" border=1>
    <tr>
      <td align=middle  width="14.28%" bgcolor=#04A872> Monday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Tuesday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Wednesday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Thursday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Friday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Saturday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Sunday </td>
    </tr> <tr>
<td><td><td><td><td></td></td></td></td></td>
     <td valign=top> 01/08

		



    </td>

     <td valign=top> 02/08
       

       
     </td>
</tr>
<tr>


     <td valign=top align=left> 03/08

<br>       
- EXSP digestion (R0010, R0080, B0034, Pt_I13507)<br><br>  
- NotI digestion (Pl_I13507)<br><br>  
- Ligation Pa_RBS (R0080 + B0034) and Pl_RBS (R0010 + B0034)<br><br>
<br>
       
     </td>
     <td valign=top align=left> 04/08
<br>

- NotI digestion (Pr_I13507)<br><br>
- Agarose gel (Pr_I13507) - Positive results!!<br><br>
- EXSP digestion (Pt_I13507)<br><br>
- Transformation (Pl_RBS and Pa_RBS)<br><br>

<br>
     </td>
     <td valign=top align=left> 05/08
<br>
- agarose gel (Pl_I13507, Pt_I13507)- negative results for Pl_13507 :(<br><br>
- Agarose gel  and gel purification (Pt_I13507)<br><br>
- ligation Pl_I13507 (Pl_P0440 + Pt_I13507)<br><br>
- inoculum (Pl_RBS, Pa_RBS)<br><br>

<br>


     </td>
 

     <td valign=top align=left> 06/08
<br>     
- Transformation (Pl_I13507, Pr_I13507)<br><br>
- miniprep (Pl_RBS, Pa_RBS)<br><br>
- NotI digestion (Pl_RBS, Pa_RBS)<br><br>

<br>

    </td>
     <td valign=top align=left> 07/08
<br>
- Agarose gel (Pl_RBS, Pa_RBS) - Only Pa_RBS was positive<br><br> 
- EXSP digestion (Pa_RBS, Pt_Term)<br><br>
- Ligation Pa_Term(Pa_RBS + Pt_Term) Pa_I13507 (Pa_RBS + GFP_I13507)<br><br>

<br>
     </td>
     
    <td valign=top align=left> 08/08
<br>
- Ligation (2X_RBS_hokD + Term)<br><br>
- Transformation (Pa_I13507, Pa_Term) <br><br>

<br>
 </td>

     <td > 09/06
<br>       
- Inoculum (Pa_I13507,Pl_I13507,Pa_Term)<br><br>
<br>
     </td>

</tr>
<tr>



     <td valign=top align=left> 10/08
<br>       
- miniprep (Pa_I13507, Pl_I13507, Pa_Term)<br><br>
- NotI digestion (Pa_I13507, Pa_Term)<br><br>
- Agarose gel (Pa_I13507, Pl_I13507, Pa_Term, Pl_RBS) - Positive results for Pa_I13507 and Pa_Term <br><br>
- Pre-inoculum of interlab study devices, BBa_K876004, and controls<br><br>
<br>

     <td valign=top align=left> 11/08
<br>       
- Vector exchange digestion (Pr_RBS, GFP_I13507, GFP_P0440, Tet_Term)<br><br>
- Ligation with pSB1C3 (Pr_RBS,  GFP_I13507, GFP_P0440, Tet_Term)<br><br>
- EXSP digestion (Pl_RBS, GFP_I13507, Pt_Term)<br><br>
- ligation (Pl_I13507, Pl_Term)<br><br>
- Transformation OmpA<br><br>
- Sample preparation of Interlab Study devices and controls (plate reader)<br><br>
- End-point measurement of Interlab Study devices and controls <br><br>
- Sample preparation of BBa_K876004 part (plate reader)<br><br>
- Kinetic measurement of BBa_K876004 (14 hours).<br><br>
<br> 
     </td>

     <td valign=top align=left> 12/08
<br>
- Transformation (Pr_RBS,  GFP_I13507, GFP_P0440, Tet_Term)<br><br>
- Inoculum OmpA<br><br>
- Pre-inoculum of interlab study devices, BBa_K876004, and controls       <br><br>
<br>
       
     <td valign=top align=left> 13/08
<br>       
- Inoculum (Pr_RBS,  GFP_I13507, GFP_P0440, Tet_Term)<br><br>
- miniprep OmpA<br><br>
- EXSP digestion (B0034, OmpA)<br><br>
- agarose gel (B0034, OmpA)<br><br>
- Ligation RBS_OmpA (RBS + OmpA)<br><br>
- Sample preparation of Interlab Study devices and controls (flow cytometry)<br><br>
- Measurement of Interlab Study devices and controls <br><br>
- Sample preparation of BBa_K876004 part  (flow cytometry)<br><br>
- Flow cytometry measurement of BBa_K876004 (14 hours).<br><br>

<br>
     </td>
    
 <td valign=top align=left> 14/08

<br>       
- miniprep (Pr_RBS, GFP_P0440(C3), Pl_I13507) - Positive results for Pl_I13507<br><br> 
- and GFP_P0440<br><br>
- Transformation RBS_OmpA<br><br>
<br>
     
     
     <td valign=top align=left> 15/08
<br>       
- Inoculum (RBS_OmpA, GFP_I13507(C3)<br><br>
<br>

       
     </td>

     <td valign=top align=left> 16/08
       
<br>
- miniprep (RBS_OmpA, GFP_I13507(C3))<br><br>
- NotI digestion (RBS_OmpA, GFP_I13507(C3))<br><br>
- Agarose gel (RBS_OmpA, GFP_I13507(C3))<br><br>
- Ligation Pl_Term(Pl_RBS + Pl_Term)<br><br>
<br>


</tr>
<tr>
       
     <td valign=top align=left> 17/08
<br>       
- Inoculum (RBS_OmpA)<br><br>
- Transformation (Pl_Term)<br><br>
<br>       


     </td>
     <td valign=top align=left> 18/08
<br>       
- IDT primers and gBlocks arrived<br><br>
- miniprep (RBS_OmpA)<br><br>
- NotI digestion (RBS_OmpA)<br><br>
- Agarose gel (RBS_OmpA) - Postive<br><br>
- Vector exchange digestion (RBS_hokD, Pr_RBS)<br><br>
- Ligation RBS_hokD(C3), Pr_RBS(C3)<br><br>
- Inoculum (Pl_Term)<br><br>
<br>   
    
     <td valign=top align=left> 19/08
<br>
- Transformation (RBS_hokD(C3), Pr_RBS(C3))<br><br>
- NotI digestion (Pl_Term)<br><br>
- Agarose gel (Pl_Term)- positive!!!<br><br>
<br>

       
     </td>
     <td valign=top align=left> 20/08       
<br>
- gBlocks and Primers resuspension and quantification<br><br>
- EP digestion (Pl_I13507, Pa_I13507, Pr_I13507, Pl_term, Pa_Term, Pr_Term)<br><br>
- Ligation Pl_I13507, Pa_I13507, Pr_I13507, Pl_term, Pa_Term, Pr_Term with pBS1C3<br><br>
- Inoculum (RBS_hokD(C3), Pr_RBS(C3))<br><br>
<br>
       
     <td valign=top align=left> 21/08
<br>       
- PCR for mutation of EcoRI roxA site<br><br>
- Agarose gel  for amplification confirmation and purification of roxA PCR mutation<br><br>
- DpnI digestion of purificated PCR and DH5a transformation using the DNA digested<br><br>
- PCR for insert amplification of Lcp inserting Biobrick sites<br><br>
- PCR for insert amplification of Lcp inserting pETSUMO restriction sites (Lcp heterologous expression)<br><br>
- Agarose gel for amplification confirmation and purification of lcp PCRs<br><br>
- Digestion of Lcp PCR biobrick and pSB1C3 with EP<br><br>
- Digestion of Lcp PCR for pETSUMO and pETSUMO with restriction enzymes BamHI and HindIII<br><br>
- miniprep (RBS_hokD(C3), Pr_RBS(C3))<br><br>
- NotI digestion (RBS_hokD(C3), Pr_RBS(C3))<br><br>
- Agarose gel (RBS_hokD(C3), Pr_RBS(C3)) - All negative<br><br>
- Transformation (Pl_I13507, Pa_I13507, Pr_I13507, Pl_term, Pa_Term, Pr_Term)<br><br>
- Pre-inoculum (Pl_I13507, Pa_I13507, Pr_I13507, interlab study devices and controls)<br><br>
<br>
     </td>
     
     <td valign=top align=left> 22/08
<br>       
- Agarose gel purification for Lcp ligations<br><br>
- Transformation of Lcp for biobrick and Lcp for pETSUMO using insert and vector purified<br><br>
- Inoculum of 6 roxA mutant colonies<br><br>
- Inoculum (Pl_I13507(C3))<br><br>
- Sample preparation (Pl_I13507, Pa_I13507, Pr_I13507, interlab study devices and controls) (plate reader) - five 96-well plates<br><br>
- Measurement of Pl_I13507, Pa_I13507, Pr_I13507, interlab study devices and controls (12 hours - plate reader)<br><br>
- Pre-inoculum (Pl_Term, Pa_Term, Pr_Term, BBa_K876004 and controls) (codes))<br><br>
<br>
    
 <td valign=top align=left> 23/08
<br>       
- gBlock reactions and transformations (linker-GFP, 6XHis, signalTAT)<br><br>
- Miniprep roxA mutants<br><br>
- Digestion of EcoRI and HindIII of roxA mutants and plasmid non-mutated (control)<br><br>
- Agarose gel of the restriction analysis<br><br>
- Sample preparation (Pl_Term, Pa_Term, Pr_Term, BBa_K876004 and controls) (plate reader) - five 96-well plates<br><br>
- Measurement of Pl_Term, Pa_Term, Pr_Term, Pl_I13507, Pa_I13507, Pr_I13507 and BBa_K876004 (12 hours - plate reader)<br><br>
- miniprep (Pl_I13507(C3))<br><br>
- NotI digestion (Pl_I13507(C3))<br><br>
- Agarose gel (Pl_I13507(C3)) - posite results<br><br>

<br>

</tr>
<tr>
       
     </td>
     <td valign=top align=left> 24/08
<br>       
- Inoculum (linker-GFP, 6XHis, signalTAT, Lcp(pETSUMO), Lcp(pSB1C3))<br><br>
- miniprep (Pa_Term, Pl_Term)<br><br>
- EP digestion (Pa_Term, Pr_Term, Pl_Term, 2xHokD, B0015)<br><br>
- PCR of mutant roxA for biobrick ligation<br><br>
<br>

       
     <td valign=top align=left> 25/08

<br>       
- miniprep (OmpA_RBS, Pr_RBS, Pr_I13507, linker-GFP, signalTAT, 6xHis, Lcp(pETSUMO), Lcp(pSB1C3))<br><br>
- NotI and EXSP digestions ( (OmpA_RBS, Pr_RBS, Pr_I13507, linker-GFP, signalTAT, 6xHis, Lcp(pETSUMO), Lcp(pSB1C3))<br><br>
- Agarose gel ((OmpA_RBS, Pr_RBS, Pr_I13507, linker-GFP, signalTAT, 6xHis, Lcp(pETSUMO), Lcp(pSB1C3)) - Positive results for OmpA_RBS ,linker-GFP, signalTAT, 6xHis, Lcp(pETSUMO), Lcp(pSB1C3)<br><br>
- Ligation 6xHis_Term (linker6xHis +B0015), GFP_Term (linkerGFP + B0015), OmpA_Lcp (OmpA + Lcp(pSB1C3)), Tat_Lcp (signalTAT + Lcp(pSB1C3)<br><br>
- Ligation (Pa_Term(C3), Pl_Term(C3), Pr_Term(C3)<br><br>
- Agarose gel (mutant RoxA)<br><br>
<br>
       
     </td>
     <td valign=top align=left> 26/08
<br>       
- Transformation 6xHis_Term, GFP_Term, OmpA_Lcp, Tat_Lcp<br><br>
<br>
     


     <td valign=top align=left> 27/08
<br>       
- Inoculum (6xHis_Term, GFP_Term, mpA_Lcp, Tat_Lcp)<br><br>
- Transformation (2xhokD_Term)<br><br>
- PCR of mutant roxA for biobrick ligation<br><br>
- Agarose gel of mutant roxA PCR product <br><br>
<br>
       
     </td>
     <td valign=top align=left> 28/08
<br>       
- roxA PCR product purification<br><br>
- roxA PCR product digestion with EP<br><br>
- miniprep (6xHis_Term, GFP_Term, OmpA_Lcp, Tat_Lcp)<br><br>
- NotI and EXSP digestions (6xHis_Term, GFP_Term, OmpA_Lcp, Tat_Lcp) - Only 6xHis_Term was negative<br><br>
- ligation Lcp_Term I (Tat_Lcp + GFP_Term), Lcp_Term II (OmpA_Lcp + GFP_Term) <br><br>
- Pre-inoculum (Pl_Term, Pa_Term, Pr_Term, Pl_I13507, Pa_I13507, Pr_I13507)<br><br>

<br>
     </td>
          
     <td valign=top align=left> 29/08
       
<br>        
- Agarose gel (roxA PCR product)<br><br>
- Ligation roxA(mutant) +pSB1C3<br><br>
- Sample preparation (Pl_Term, Pa_Term, Pr_Term, Pl_I13507, Pa_I13507, Pr_I13507) (plate reader) - seven 96-well plates<br><br>
- Measurement of test promoters (Pl_Term, Pa_Term, Pr_Term, Pl_I13507, Pa_I13507, Pr_I13507)<br><br>
<br>
     </td>
     <td valign=top align=left> 30/08
<br>       
- Transformation (roxA)<br><br>
<br>

</tr>
<tr>       
     </td>
     <td valign=top align=left> 31/08
<br>
- Inoculum (roxA)<br><br>
- Transformation (Lcp_Term I, Lcp_Term II)<br><br>
<br>
       
        
       
     </td>
     
   </table>
   
       <h1>September</h1>


    <table align=center width="100%" border=1>
    <tr>
      <td align=middle  width="14.28%" bgcolor=#04A872> Monday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Tuesday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Wednesday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Thursday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Friday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Saturday </td>
      <td align=middle  width="14.28%" bgcolor=#04A872> Sunday </td>
    </tr> <tr>

<td></td>

     <td valign=top> 01/09

		<br>
                 - miniprep (roxA)<br><br>
                 - Inoculum (Lcp_Term I)<br><br>
                 - Ligation  (Lcp_Term II)<br><br>
                 <br>



    </td>
     <td valign=top> 02/09
       
<br>        	
- miniprep (Lcp_Term I)<br><br>
- digestion (Lcp_Term I, roxA))<br><br>
- Transformation  (Lcp_Term II)<br><br>
<br>
       
     </td>
     <td valign=top align=left> 03/09

<br>       
- Transformation (Pr_RBS, 2X_RBS_hokDLigation Pl_hokD (Pl + 2xhokD))<br><br>
-Pre-inoculum (Pl_Term, Pa_Term, Pr_Term, Pl_I13507, Pa_I13507, Pr_I13507)<br><br>
<br>
       
     </td>
     <td valign=top align=left> 04/09
<br>
- Transformation (Pl_2xhokD)<br><br>
-Sample preparation of test promoters circuits (plate reader)<br><br>
- Measurement of test promoters circuits (Pl_Term, Pa_Term, Pr_Term, Pl_I13507, Pa_I13507, Pr_I13507)<br><br>
<br>
     </td>
     <td valign=top align=left> 05/09
<br>
- Inoculum (Pl_2xhokD)<br><br>
<br>


     </td>
 

     <td valign=top align=left> 06/09
<br>     
- Transformation in Bl21(DE3) (Pl_I13507, Pa_I13507, Pr_I13507, Pl_Term, Pa_Term, Pr_Term)<br><br>
<br>

    </td>

</tr><tr>
     <td valign=top align=left> 07/09
<br>
- Inoculum (Pl_I13507, Pa_I13507, Pr_I13507, Pl_Term, Pa_Term, Pr_Term, in BL21(DE3))<br><br>
<br>
     </td>
     
    <td valign=top align=left> 08/09
<br>
- Miniprep (Pl_I13507, Pa_I13507, Pr_I13507, Pl_Term, Pa_Term, Pr_Term, in BL21(DE3))<br><br>
- Digestion with SacI (OmpA_Lcp, OmpA_roxA, TAT_Lcp, TAT_roxA)<br><br>
- Digestion with SacI and XbaI (OmpA, signalTAT, Lcp, RoxA)<br><br>
- EXSP digestion (R0010, R0080, K914003)<br><br>
-NotI digestion (Pl_I13507, Pa_I13507, Pr_I13507, Pl_Term, Pa_Term, Pr_Term, in BL21(DE3))<br><br>
- Agarose gel (OmpA_Lcp, OmpA_roxA, TAT_Lcp, TAT_roxA, OmpA, signalTAT, Lcp, RoxA, Pl_I13507, Pa_I13507, Pr_I13507, Pl_Term, Pa_Term, Pr_Term) - Positive results for Pl_I13507 and Pr_I13507)<br><br>
- Ligation OmpA_Lcp, OmpA_roxA, TAT_Lcp, TAT_roxA, Pl_I13507II (R0010 + GFP_I13507), Pl_TermII (R0010 + tet_Term), Pa_I13507II (R0080 + GFP_I13507), Pa_TermII (R0080 + tet_Term), Pr_I13507II (K914003 + GFP_I13507), Pr_TermII (K914003 + tet_Term),<br><br>
-Transformation (OmpA_Lcp, OmpA_roxA, TAT_Lcp, TAT_roxA, Pl_I13507II, Pa_I13507II, Pr_I13507II, Pl_TermII, Pa_TermII, Pr_TermII)<br><br>
<br>
 </td>
     <td valign=top align=left> 09/09
<br>       
-Western blot to see if LCP was been expressed in pETSUMO<br><br>
- inoculum (Pl_I13507II, Pa_I13507II, Pl_TermII, Pa_TermII, Pr_TermII, TAT_Lcp_RBS, OmpA_Lcp_RBS, OmpA_roxA_Pt)<br><br>
- Prepare plates for characterization (Pl_I13507 and Pr_I13507 in BL21(DE3)) 10th<br><br>
<br>
     </td>
     <td valign=top align=left> 10/09
<br>       
- Miniprep (Pl_I13507II, Pa_I13507II, Pl_TermII, Pa_TermII, Pr_TermII,  TAT_Lcp_RBS, OmpA_Lcp_RBS, OmpA_roxA_Pt)<br><br>
-NotI digestion (Pl_I13507II, Pe_I13507II, Pl_TermII, Pa_TermII, Pr_TermII)<br><br>
- SacI digestion again (OmpA_Lcp, OmpA_roxA, TAT_Lcp, TAT_roxA,  TAT_Lcp_RBS, OmpA_Lcp_RBS, OmpA_roxA_Pt)<br><br>
-Agarose gel (Pl_I13507II, Pe_I13507II, Pl_TermII, Pa_TermII, Pr_TermII, OmpA_Lcp, OmpA_roxA, TAT_Lcp, TAT_roxA,  TAT_Lcp_RBS, OmpA_Lcp_RBS, OmpA_roxA_Pt)<br><br>
-Ligation (OmpA_Lcp, OmpA_roxA, TAT_Lcp, TAT_roxA,  TAT_Lcp_RBS, OmpA_Lcp_RBS, OmpA_roxA_Pt, GFP_TermA (J23101 + linkerGFP_Term), GFP_TermB (J23117 + linkerGFP_Term), GFP_TermC (J23106 + linkerGFP_Term),<br><br>
-Transformation (OmpA_Lcp, OmpA_roxA, TAT_Lcp, TAT_roxA,  TAT_Lcp_RBS, OmpA_Lcp_RBS, OmpA_roxA_Pt, GFP_TermA, GFP_TermB, GFP_TermC<br><br>
<br>

     <td valign=top align=left> 11/09
<br>       
- Transformation (TAT_ lcp_RBS, TAT_ lcp, TAT_ roxA, OmpA_roxA_Pt)<br><br>
-Inoculum (GFP_TermA, GFP_TermB, GFP_TermC)<br><br>
<br> 
     </td>

     <td valign=top align=left> 12/09
       <br>       
- Miniprep (GFP_TermA, GFP_TermB, GFP_TermC)<br><br>
- Not digestion (GFP_TermA, GFP_TermB, GFP_TermC)<br><br>
- Xbal and NotI digestion (RoxA, signalTAT and Lcp)<br><br>
-Agarose gel (GFP_TermA, GFP_TermB, GFP_TermC, RoxA, signalTAT and Lcp)<br><br>
-Ligation TAT_Lcp (signalTAT + Lcp), TAT_RoxA (signalTAT + roxA)<br><br>
<br>
       
     <td valign=top align=left> 13/09
<br>       
- Transformation (TAT_Lcp, TAT_roxA)<br><br>
<br>
     </td>
</tr><tr>
    
 <td valign=top align=left> 14/09

<br>       
- EP digestion to change the vector  (Pa_Term,  signalTAT, OmpA, Lcp, RoxA)<br><br>
- SacI and EcoRI digestion (signalTat, OmpA)<br><br>
- SacI and PstI digestion (Lcp, RoxA)<br><br>
- Agarose gel ( Pa_Term,  signalTAT, OmpA, Lcp, RoxA)<br><br>
-Ligation (pSB1A2 + RoxA, pSB1A2 + Lcp, pSB1A2 + OmpA and pSB1A2 + SignalTAT)<br><br>
-3A assembly (TAT_RoxA(pSB1A2 +signalTA+RoxA), TAT_Lcp(pSB1A2 +signalTA+Lcp), OmpA_RoxA(pSB1A2 +OmpA+RoxA),OmpA_Lcp( pSB1A2 +OmpA+Lcp))<br><br>
-Transformation (RoxA, Lcp, OmpA, SignalTAT, TAT_RoxA, TAT_Lcp, OmpA_RoxA, OmpA_Lcp)<br><br>
- Inoculum (linkerGFP_J23117, linkerGFP_J23106)<br><br>
<br>
     
     
     <td valign=top align=left> 15/09
<br>       
- Ligation again (pSB1A2 + RoxA, pSB1A2 + Lcp, pSB1A2 + OmpA and pSB1A2 + SignalTAT)<br><br>
- 3A assembly again (TAT_RoxA(pSB1A2 +signalTA+RoxA), TAT_Lcp(pSB1A2 +signalTA+Lcp), OmpA_RoxA(pSB1A2 +OmpA+RoxA),OmpA_Lcp( pSB1A2 +OmpA+Lcp))<br><br>
- Sample preparation (linkerGFP_J23117, linkerGFP_J23106)<br><br>
- Measurements (linkerGFP_J23117, linkerGFP_J23106)<br><br>
<br>

       
     </td>
     <td valign=top align=left> 16/09
       
<br>
- Transformation (RoxA, Lcp, OmpA, SignalTAT, TAT_RoxA, TAT_Lcp, OmpA_RoxA, OmpA_Lcp)<br><br>
- Prepare the plates to submit the new parts<br><br>
- Lcp Expression using pETSUMO in Bl21(DE3)<br><br>
<br>

       
     <td valign=top align=left> 17/09
<br>       
- Inoculum (RoxA, Lcp, OmpA, SignalTAT, TAT_RoxA, TAT_Lcp, OmpA_RoxA, OmpA_Lcp)<br><br>
- Cell lyses<br><br>
- Western blot (Lcp)<br><br>

<br>       


     </td>
     <td valign=top align=left> 18/09
<br>       
- Miniprep (RoxA, Lcp, OmpA, SignalTAT, TAT_RoxA, TAT_Lcp, OmpA_RoxA, OmpA_Lcp)<br><br>
- NotI digestion (RoxA, Lcp, OmpA, SignalTAT, TAT_RoxA, TAT_Lcp, OmpA_RoxA, OmpA_Lcp)<br><br>
- Agarose gel (RoxA, Lcp, OmpA, SignalTAT, TAT_RoxA, TAT_Lcp, OmpA_RoxA, OmpA_Lcp)<br><br>
- Western blot (Lcp)<br><br>
<br>       
     
   </table>



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