View source for Team:UCLA/Notebook/Interlab Study/2 July 2015


* Resuspended BioBrick plamids.
**Resuspended BioBricks BBa_J23101, BBa_J23106, BBa_J23117, BBa_I3504, BBa_I270260, and BBa_R0040 in 10uL of nuclease free ddH2O, according to registry instructions.  Aliquots were incubated at room temp in the wells for 5 minutes to reabsorb all dried DNA, and transferred to PCR tubes, labeled, and stored at -20 degrees Celsius. 

* Transformed all BioBrick plasmids in 5-alpha electrocompetent E. coli.
** Conducted using the protocol listed here.
** With the exception of BBa_I3504, all transformed cell lines were grown on chloramphenicol supplemented plates. (504 requires ampicillin ABX). 
** 200uL and 50uL aliquots of each of the 1mL rescued cells were plates using sterile glass beads on each plate, respectively.
** Plates were inverted, labeled, and incubated for 16 hours in 37 degrees Celsius. 
*** Results of the transformations are HERE. 




{{Template_All_Teams}}

<!-- Declare that you are going to use html code instead of wiki code -->
<html>

	<!-- Start of CSS-->
		<style type="text/css">

		/* PAGE LAYOUT */

		/* Change Background color*/
			body { 
				background-color: #fff;
			}

		/* Creates a container that will wrap all of the content inside your wiki pages. */
			#mainContainer {  
				width: 978px;
				overflow:hidden;
				float:left;
				margin-left:20px;
				margin-bottom: 10px;
				background-color: #fff; 
				border-bottom: 14px solid #565656;
				border-right: 2px solid #565656;
				border-left: 2px solid #565656;
				border-top: 2px solid #565656;
				font-family: "Trebuchet MS", Helvetica, sans-serif;
			}

		/* Creates the container for the menu */
			#menuContainer  { 
				float:left;
				width: 134px;
				padding: 20px 0px;
				border-top: 14px solid #565656;
				background-color: #E8E8E9;
			}

		/* Creates the container for the content */
			#contentContainer {
				padding-top:20px;
				padding-right:10px;
				margin-bottom: 20px;
				width: 814px;
				padding-left: 20px;
				float: left;
				background-color: #fff; 
				border-top: 14px solid #565656;
				font-family: "Trebuchet MS", Helvetica, sans-serif;
			}

		/*Set up height place holder for the banner*/
			#bannerContainer {
				height:200px;
				margin:auto;
				text-align:center;
                                background-image: url("https://static.igem.org/mediawiki/2015/4/48/Team_UCLA_banner.jpg");
                                background-size: cover;
				color: #24B694;
			}

		/*VISUAL STYLE (TABLES, FONTS, LINKS, IMAGES, UL) */

		/*Change the styling of text for everything inside main container*/
			#mainContainer p { 
				font-size: 13px;
				color: #000000;
			}
			
		/*This changes the color and font family */
			#contentContainer h1, h2, h3, h4, h5, h6 { 
				color: #565656;
				border-bottom: none;
				font-weight: bold;
				font-family: "Trebuchet MS", Helvetica, sans-serif;
				margin-top:10px;
			}

		/*Style of the links - links are different inside the menu */
			#contentContainer a { 
				font-weight: bold;
				color: #23b593;
			}

	 	  /* Styling links on hover- links are different inside the menu */
			#contentContainer a:hover { 
				color: #59bf92;
			}

		/*Change the styling of tables */
    	    		#contentContainer table {
    				border: 1px solid #565656;
    				border-collapse: collapse;
    				width: 90%
    				margin: auto;
    				margin-bottom: 15px;
    				margin-top: 15px;
    				margin-right: 10px;
    				margin-left: 10px;
    			}

		/*Change the styling of table cells*/
    			#contentContainer  td {
    				padding: 10px;
    				border: 1px solid #565656;
    				border-collapse: collapse;
    				vertical-align: text-top;
    			}

		/*Change the styling of table headers */
    			#contentContainer th {
    				background-color: #E8E8E9;
    				padding: 10px;
    				border: 1px solid #565656;
    				border-collapse: collapse;
    				vertical-align: text-top;
    			}



		/*MENU STYLING */

		/*Styling for the links in the menu */
			#menuContainer a {
				color: #565656;
				text-decoration:none;
				font-weight: bold;
			}

		/* Sets the style for lists inside menuContainer  */
			#menuContainer ul { 
				list-style: none;
				margin-left:0px;
			}

		/*Styles the list items to become menu buttons */
			#menuContainer ul li { 
				text-align: center;
				display: block;
				width: 100%;
				height:30px;
				padding-top:10px;
			}

		/*For the menu buttons, changes the color when hovering*/
			#menuContainer li:hover {
				color:  #FFF;
				background-color: #24B694;
			}

		/*Submenus are not displayed as default*/
			#menuContainer li ul {
				display: none;
				padding-top:15px;
				margin-left: -19px;
			}

		/*Submenus are displayed when hovering the menu button */
			#menuContainer li:hover ul {
				/*display: inline-block; */
				display: block; 
				position: absolute; 
				float:right;
				margin-left: 134px;
				margin-top:-42px;
 			}

		/*Style the submenu buttons*/
			#menuContainer li ul li{
				background-color: #59BF92; 
				padding-left:20px;
				padding-right:20px;
				height:30px;
				padding-top:10px;
                                padding-bottom:10px;
				margin-top:-2px;
				color: #565656;
				width: 150px;
			}

	/*CLASSES */

		/*Clear class for all the pages, adds spacing too*/
			.clear{
				clear:both;
				height: 10px;
			}


		/* highlight box for special messages */
    			.highlightBox {
    			    	width:500px; 
    			    	margin:auto; 
    			    	background-color: #E8E8E9; 
				margin-bottom: 15px;
				margin-top: 15px;
				padding: 15px;
				padding-top: 5px;
    			 }


		</style>
	<!-- End of CSS -->


		<!-- Start of the template html elements. -->
		<div id ="mainContainer"><!--The closing tag for mainContainer should be placed at the bottom of each content page.-->


			<div id="bannerContainer">
			</div>

			<!-- Start of menu -->
			<div id="menuContainer">

				<!-- This list is your menu, every list item is a menu button and nested listed become submenu buttons -->
				 <ul>
					<a href="https://2015.igem.org/Team:UCLA"><li>HOME</li></a>

					<a href="https://2015.igem.org/Team:UCLA/Team"><li>TEAM</li></a>

					<a href="#"><li>PROJECTS
            					<ul>
<!--							<a href="https://2015.igem.org/Team:UCLA/Description"><li>Description</li></a>
							<a href="https://2015.igem.org/Team:UCLA/Experiments"><li>Experiments &amp; Protocols</li></a>  
							<a href="https://2015.igem.org/Team:UCLA/Results"><li>Results</li></a>  
							<a href="https://2015.igem.org/Team:UCLA/Design"><li>Design</li></a>-->
							<a href="https://2015.igem.org/Team:UCLA/Projects/Silks"><li>Silk Genetics</li></a>
							<a href="https://2015.igem.org/Team:UCLA/Projects/Functionalization"><li>Functionalization</li></a>
							<a href="https://2015.igem.org/Team:UCLA/Projects/Materials"><li>Materials Processing</li></a>
							<a href="https://2015.igem.org/Team:UCLA/Projects/Protein_Cages"><li>Protein Cages</li></a>
						</ul>
					</li></a>

					<a href="#"><li>PARTS
            					<ul>
							<a href="https://2015.igem.org/Team:UCLA/Parts"><li>Team Parts</li></a>
							<a href="https://2015.igem.org/Team:UCLA/Basic_Part"><li>Basic Parts</li></a>  
							<a href="https://2015.igem.org/Team:UCLA/Composite_Part"><li>Composite Parts</li></a>
							<a href="https://2015.igem.org/Team:UCLA/Part_Collection"><li>Part Collection</li></a>  
						</ul>
					</li></a>

					<a href="https://2015.igem.org/Team:UCLA/Notebook"><li>NOTEBOOKS
                                                <ul>
							<a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics"><li>Spider Silk Genetics</li></a>
							<a href="https://2015.igem.org/Team:UCLA/Notebook/Honeybee_Silk"><li>Honeybee Silk</li></a>  
							<a href="https://2015.igem.org/Team:UCLA/Notebook/Recombinant_Expression"><li>Recombinant Silk Functionalization</li></a>  
							<a href="https://2015.igem.org/Team:UCLA/Notebook/Materials"><li>Materials  Processing</li></a>  
							<a href="https://2015.igem.org/Team:UCLA/Notebook/Protein_Cages"><li>Protein Cages</li></a>
                                                        <a href="https://2015.igem.org/Team:UCLA/Notebook/Interlab_Study"><li>UCLA Measurement Interlab Study</li></a>
						</ul>
                                        </li></a>

					<a href="#"><li>MEETING NOTES
                                                <ul>
							<a href="https://2015.igem.org/Team:UCLA/Notebook/General_Meetings"><li>General Meetings</li></a>
                                                        <a href="https://2015.igem.org/Team:UCLA/Notebook/Coordinator_Meetings"><li>Coordinator Meetings</li></a>
							<a href="https://2015.igem.org/Team:UCLA/Notebook/Advisor_Meetings"><li>Advisor Meetings</li></a>    
						</ul>
                                        </li></a>

       
					<a href="https://2015.igem.org/Team:UCLA/Attributions"><li>ATTRIBUTIONS</li></a>

					<a href="https://2015.igem.org/Team:UCLA/Collaborations"><li>COLLABORATIONS</li></a>

					<a href="https://2015.igem.org/Team:UCLA/Practices"><li>HUMAN PRACTICES</li></a>

					<a href="https://2015.igem.org/Team:UCLA/Safety"><li>SAFETY</li></a>

					<a href="https://2015.igem.org/Team:UCLA/Modeling"><li>MODELING</li></a>

					<a href="https://2015.igem.org/Team:UCLA/Measurement"><li>MEASUREMENT STUDY</li></a>

					<a href="https://2015.igem.org/Team:UCLA/Software"><li>SOFTWARE</li></a>

					<a href="https://2015.igem.org/Team:UCLA/Entrepreneurship"><li>ENTREPRENEURSHIP</li></a>

				 </ul>
			</div>
			<!-- End of menu  -->

			<!-- Start of content -->
			<div id="contentContainer"> <!--The closing tag for contentContainer should be placed at the bottom of each content page.-->
</html>