View source for Team:BostonU

{{Team:BostonU}}
<html>
<header>
<title>Home</title>
<style type="text/css">
/*
#header {
  margin-top: 150px;
    height: 265px;
    background: url(https://static.igem.org/mediawiki/2015/0/08/Homepic.JPG) no-repeat;
    background-repeat: no-repeat;
    background-position: center; 
}
#contentpage {
    border-color: #C0C0C0;
    background-color: #DBDBFF;
    width: 80%;
    margin-right: auto;
    margin-left: auto;
    border-radius: 10px;
    font-weight: bold;
    font-size: 16px;
}
#mainpage {
    border: 1px solid #C0C0C0;
    width: 100%;
    float: left;
    padding: 10px;
    height: 300px;
    box-shadow: 5px 5px 2px #888888;
    border-radius: 10px;
    background-color: #DBDBFF;
    font-family: Arial, Helvetica, sans-serif;
}
#mainpage p {
	line-height:22px;
	margin:10px 0px 22px 0px;
}
#mainpage blockquote {
	background:#DBDBFF;
	display:block; 
	margin:5px;
	font family: Arial, Helvetica, sans-serif;	
        font-style:italic;
}
h2, h3, h4, h5 {
    color: #000000;
    font-weight: bold;
    font-family: Arial, Helvetica, sans-serif;
    vertical-align: middle;
    font-size: 30px;
}
.clear {
	clear:both;}

*/
    </style>


</header>
<body bgcolor="#DBDBFF">
<div id="wrapper">

<div id="header">
</div>


<div id="contentpage">
<div id="mainpage">
<h2 style="font-color:#000000;">Developing conditionally dimerizable split protein systems for genetic logic and genome editing applications </h2>

<p style="padding-bottom:100px;">The field of synthetic biology seeks to engineer desirable cellular functionalities by developing molecular technologies that enable precise genetic manipulation. A promising solution is to reliably control proteins that naturally execute genetic modifications. Current strategies to regulate activity of such proteins primarily rely on modulating protein expression level through transcriptional control; however, these methods are susceptible to slow response and leaky expression. In contrast, strategies that exploit post-translational regulation of activity, such as conditional dimerization of split protein halves, have been demonstrated to bypass these limitations. Here, we compare the relative efficiency of previously characterized dimerization domains in regulating activities of three important genetic manipulation proteins - integrases and recombination directionality factors for genetic logic applications, and saCas9 for in vivo genome editing applications. We also establish guidelines to rationally identify promising protein split sites. Our characterization of these systems in mammalian cells ultimately paves way for important biomedical applications.</p>

<center>
<table align="center">
<tr>
<td><center><a href="https://2015.igem.org/Team:BostonU/Temporal_Control"><img style="height:55%; width:55%; padding-right:30px;" src="https://static.igem.org/mediawiki/2015/thumb/9/99/Active_and_inactive_protein.png/800px-Active_and_inactive_protein.png" /></a></center></td>
<td><center><a href="https://2015.igem.org/Team:BostonU/App_1/Motivation"><img style="height:55%; width:55%; padding-left:30px; padding-right:30px;" src="https://static.igem.org/mediawiki/2015/thumb/8/86/Home_page_integrase_RDF.png/562px-Home_page_integrase_RDF.png" /></a></center></td>
<td><center><a href="https://2015.igem.org/Team:BostonU/App_2/Motivation"><img style="height:55%; width:55%; padding-left:30px;" src="https://static.igem.org/mediawiki/2015/thumb/1/1c/Crispr_cas9_labeled.png/800px-Crispr_cas9_labeled.png" /></a></center></td>
</tr>

<tr>
<td><h3 align="center" style="padding-bottom:50px;">Controlling Protein Activity</h3></td>
<td><h3 align="center" style="padding-bottom:50px;">Integrase and RDF</h3></td>
<td><h3 align="center" style="padding-bottom:50px;">saCas9</h3></td>

</tr>

<tr>
<td><center><a href="https://2015.igem.org/Team:BostonU/Mammalian_synbio/Significance"><img style="height:40%; width:40%; padding-right:30px;" src="https://static.igem.org/mediawiki/2015/thumb/5/59/Mammalian_syn_bio_home_page.png/618px-Mammalian_syn_bio_home_page.png" /></a></center></td>
<td><center><a href="https://2015.igem.org/Team:BostonU/Education/Building_with_Biology"><img style="height:75%; width:75%; padding-left:30px; padding-right:30px;" src="https://static.igem.org/mediawiki/2015/c/c5/Building_w_biology.jpg" /></a></center></td>
<td><center><a href="https://2015.igem.org/Team:BostonU/Attributions"><img style="height:55%; width:55%; padding-left:30px;" src="https://static.igem.org/mediawiki/2015/thumb/b/ba/Transcriptional_logic_circuit.png/800px-Transcriptional_logic_circuit.png" /></a></center></td>
</tr>

<tr>
<td><h3 align="center">Mammalian Synthetic Biology</h3></td>
<td><h3 align="center">Education and Outreach</h3></td>
<td><h3 align="center">Attributions</h3></td>
</tr>
</table>
</center>


<br><br><br><br><br><br>

</body>
</html>