View source for Team:HAFS-Korea/Safety

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Safety Procedures for Experiment</h1>
<ol><li>When using the alcohol lamp to sterilize the atmosphere, take caution especially when putting the cap back on to extinguish the flames.</li>
<li>Be sure to wear gloves during the experiment to prevent contamination and to prevent harmful substances from making contact with the skin.</li>
<li>When checking the process of electrophoresis, do not hold the gel; instead, use a spatula to put the gel into the carcinogenic substance, Ethidium bromide.</li>
<li>When cutting the Agarose gel, be careful of using the blade and do not press the blade with bare hands.
<br> → after extraction(by cutting gel band at 1.4kb) →</li> 
<li>Be careful of the heat when placing the DNA tubes into the PCR machine (temperatures tend to rise to over 95 degrees celsius).</li></ol>

<h3>Tips we learned from lab. Survive in the lab for high school kids</h3>
<ol><li>Do not wander around when pipet is covered with tip</li>
<li>Set the maximum levels after using pipet</li>
<li>When tips are used for same solution, they don’t need to be replaced</li></ol>

<h4>@ When treating micro organism in front of the alcohol lamp</h4>
<p>Whenever treating micro organism, heat until the white gold gets red enough. With plates, manage the distance appropriately because the far distance could make easily entrance into the plates</p>
<h4>@ Centrifuge</h4>
<p>Always manage the balance, or else the cacophony will occur with high probability of eruption</p>
<h4>@Vortex</h4>
<p>Do short centrifuge to use the liquid on top of tube (use small centrifuge machine) for 10 seconds-> make sure all the liquids of the micro tube is used</p>
<h4>@ inverting</h4>
<p>= turn up side down of the tube (make sure you slightly exert your strength)</p>
<h4>@Extracting plasmid(read dna mini prep kit with protocol)</h4>
<ul><li>Make sure you take up all the upper liquid (but make sure you don’t touch the bottom debris)</li>
<li>The next step of cell lysis have to be done in three minutes(not intense mixing because the liquid can destroy the plasmid)</li>
<li>The reason why we are doing vortex is because we eliminate all the ethanol</li>
<li>In the elution step, we should make sure that buffer is pull through the white area</li>


@trichoderma extraction step
-       Before the experiment we ought to set the incubation temperature
-       Pick up the fungus in the plate then add distilled water and use the vortex machine. Mix it long enough(about 12 minutes, but do not over-vortex)



@electrophoresis
-       In the part of gel running, use the pipet appropriate for 2 micro liter
-       Make the three spot using the blue coloring matter
-       Mix the liquid enough and fill them to the spot of the gel(make sure all the liquid set in their own site)




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