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		<div class="cd-timeline-block" id="01-07-15">
			<div class="cd-timeline-content">
				<div class="cd-timeline-img cd-picture"></div>

				<center>
					<h3> <!-- Titre general de l'experience du jour -->
						PCR pDawn BioBricks
					</h3>
					<h4> <!-- Sous partie de l'experience -->
						PCR pDawn III
					</h4>
				</center>
				<h5>
					<i>  <!-- Objectif de l'experience -->
						Aim: first step for pDawn amplification and mutagenesis
					</i>
				</h5>
				
				
				<table id="PCRmix">
					<caption valign="top" style="color:rgba(255, 0, 0, 0.6);font-weight: bold;"> Mix PCR preparation:</caption>
	
					<tr>
						<th>
							
						</th>
						<th> 
							pDawn III
						</th>
					</tr>
	
				<tr>
						<td>
							MQ water
						</td>
						<td> 
							39,5 µL
						</td>
					</tr>
					
					<tr>
						<td>
							Mg2+ 50 mM
						</td>
						<td> 
							1,5 µL
						</td>
					</tr>
					
					<tr>
						<td>
							Buffer RB
						</td>
						<td> 
							5 µL
						</td>
					</tr>
					
					<tr>
						<td>
							dNTPs 10µL
						</td>
						<td> 
							1 µL
						</td>
						
						<tr>
						<td>
							Primer Fwd 50µM
						</td>
						<td> 
							1µL pDawn Fwd III
						</td>
					</tr>
					
					<tr>
						<td>
							Primer Rev 50µM
						</td>
						<td> 
							1µL pDawn Rev III
						</td>
					</tr>
					
					<tr>
						<td>
							DNA
						</td>
						<td> 
							1µL pDawn
						</td>
					</tr>
					
					<tr>
						<td>
							Taq Pol
						</td>
						<td> 
							0.5 µL 
						</td>
					</tr>
					
					
					</table>
				
					<table id="iGEMTAQ2">
					<caption valign="top" style="color:rgba(255, 0, 0, 0.6);font-weight: bold;"> PCR cycle – IGEM TAQ 2</caption>
				
					<tr>
						<th>
							
						</th>
						<th> 
							T°C
						</th>
						<th> 
							Time
						</th>
						<th> 
							Cycle
						</th>
					</tr>

					<tr>
						<td >
							Initial denaturation
						</td>
						<td><center>
							95
						</center></td>
						<td><center>
							30 sec
						</center></td>
						<td><center>
							1
						</center></td>
					</tr>
					
					<tr>
						<td >
							Denaturation
						</td>
						<td><center>
							95
						</center></td>
						<td><center>
							30 sec
						</center></td>
						<td rowspan=3><center>
							17
						</center></td>
					</tr>
					
					<tr>
						<td >
							Annealing
						</td>
						<td><center>
							60
						</center></td>
						<td><center>
							30 sec
						</center></td>
						
					</tr>
					
					<tr>
						<td >
							Extension
						</td>
						<td><center>
							72
						</center></td>
						<td><center>
							2 min 
						</center></td>
						
					</tr>
					
					<tr>
						<td >
							Final extension
						</td>
						<td><center>
							72
						</center></td>
						<td><center>
							5 min 
						</center></td>
						<td><center>
							1
						</center></td>
					</tr>
					
					<tr>
						<td>
							Hold
						</td>
						<td><center>
							4
						</center></td>
						<td><center>
							Infinite
						</center></td>
						<td><center>
							
						</center></td>
					</tr>

				</table>
				

			<center>
					<h4> <!-- Sous partie de l'experience -->
						Gibson Assembly
					</h4>
				</center>
				<h5>
					<i>  <!-- Objectif de l'experience -->
						Aim: first step of our VVD Biobrick
					</i>
				</h5>
			


<table>
					<caption valign="top" style="color:rgba(255, 0, 0, 0.6);font-weight: bold;"> Quantification of DNA fragments by Image J:</caption>
				
					<tr>
						<th>
							Fragments
						</th>
						<th> 
							Concentration (ng.µL<sup>-1</sup>)
						</th>
						<th>
							Lenght
						</th>
						<th>
						Molecular weight (g.mol<sup>-1</sup>)
						</th>
						<th>
						Molarity (mol.L<sup>-1</sup>)
						</th>
						<th>
						1/ratio
						</th>
						<th>
						Dilution factor
						</th>
						<th>
						Molarity after dilution
						</th>
						<th>
						DNA Volume
						</th>
						<th>
						Volume Water 
						</th>
						<th>
						Volume totale
						</th>
					</tr>
					<tr>
						<td>
							pSB1C3
						</td>
						<td> <center>
							91.11
						</center></td>
						<td><center>
							2 200
						</center></td>
						<td><center>
						1 430 000
						</center></td>
						<td>
						0.000000063712
						</td>
						<td><center>
						7.75
						</center></td>
						<td><center>
						1.35
						</center></td>
						<td>
						0.000000047103
						</td>
						<td><center>
						3.50
						</center></td>
						<td><center>
						1.50
						</center></td>
						<td><center>
						5
						</center></td>
					</tr>
					
					<tr>
						<td>
							VVD1
						</td>
						<td> <center>
							15.71
						</center></td>
						<td><center>
							250
						</center></td>
						<td><center>
						162 500
						</center></td>
						<td>
						0.000000096698
						</td>
						<td><center>
						5.10
						</center></td>
						<td><center>
						2.05
						</center></td>
						<td>
						0.000000047103
						</td>
						<td><center>
						1.00
						</center></td>
						<td><center>
						1.00
						</center></td>
						<td><center>
						2
						</center></td>
					</tr>
					
					<tr>
						<td>
							VVD2
						</td>
						<td> <center>
							7.65
						</center></td>
						<td><center>
							250
						</center></td>
						<td><center>
						162 500
						</center></td>
						<td>
						0.000000047103
						</td>
						<td><center>
						10.48
						</center></td>
						<td><center>
						1.00
						</center></td>
						<td>
						0.000000047103
						</td>
						<td><center>
						1.00
						</center></td>
						<td><center>
						0
						</center></td>
						<td><center>
						1
						</center></td>
					</tr>
					
					<tr>
						<td>
							YC155
						</td>
						<td> <center>
							128.31
						</center></td>
						<td><center>
							400
						</center></td>
						<td><center>
						260 000
						</center></td>
						<td>
						0.000000493488
						</td>
						<td><center>
						1.00
						</center></td>
						<td><center>
						10.48
						</center></td>
						<td>
						0.000000047103
						</td>
						<td><center>
						1.00
						</center></td>
						<td><center>
						9.00
						</center></td>
						<td><center>
						10
						</center></td>
					</tr>
					</table>
								
				<p>
						<i class="fa fa-angle-right"></i> DNA mix:
						<ul>
							<li><b>YC155</b>: 1µL + 9µL of MQ water</li>
							<li><b>VVD1</b>: 1µL + 1µL of MQ water</li>
							<li><b>VVD2</b>: no dilution</li>
							<li><b>pSB1C3</b>: 3.5µL + 1.5µL of MQ water</li>
						</ul>
						<i class="fa fa-angle-right"></i> Mix = 2µL of each
						<br>
						<i class="fa fa-angle-right"></i> 5µl used for the Gibson assembly added to 15µl of master mix
					</p>
				
			
				
		
			
			<center>
					<h4> <!-- Sous partie de l'experience -->
						Electrophoresis 
					</h4>
				</center>
				
				<p>
					
					<br>
					<i class="fa fa-angle-right"></i> Gel : 1,2 g agarose + 120 mL of TAE 1x + 150 µL of BET
					<br>
					<i class="fa fa-angle-right"></i> Migration of pDawn III
				</p>
			<div class="row">
					<div class="col-sm-6">
						<h5 style="color:rgba(255, 0, 0, 0.6);">Expected results</h5>		
								<p align="center">
									<img src="https://static.igem.org/mediawiki/2015/6/64/Expectedresultats-010715.png" alt="Expected results"  width=auto height="180">
								</p>
					</div>
					
					<div class="col-sm-6">
							<h5 style="color:rgba(255, 0, 0, 0.6);">Results </h5>
							<p align="center"> 
								<img src="https://static.igem.org/mediawiki/2015/f/fb/Resultats-010715.jpg" alt="Results"  width=auto height="180">
							</p>
					</div>   
			</div>	
				
				<p>
				<i class="fa fa-angle-right"></i>Soft band at 1500 bp, good but not enough
				
				</p>	
				
			
			
			
			
			<!-- DATE de l'experience !ID aussi -->
			<span class="cd-date" id="01-07-15"><br>1 July 15</span>
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