View source for Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Ligation with BBa K823023 and BBa K823024 (t32)

{{Team:Groningen/TEMPLATES/LOG
	|title=00:00, 9 June 2015 - 00:00, 9 June 2015
	|executor=Harm Ruesink
	|content=
<div class="text">The ligation of tasA in the plasmids (BBa_K823023 & BBa_K823024) was performed by combining the following components.</div>

<div class="object data" id="tbl1">
	<div class="wrapper">
	<div class="header">
		<div class="field fw2">Sample</div>
		<div class="field fw3">Component</div>
		<div class="field fw3">Amount</div>
	</div>
	<div class="record">
		<div class="field fw2">23 lig</div>
		<div class="field fw3">insert (tasA dig)</div>
		<div class="field fw3">10 µL</div>
	</div>
	<div class="record">
		<div class="field fw2"></div>
		<div class="field fw3">vector(23 dig)</div>
		<div class="field fw3">5 µL</div>
	</div>
	<div class="record">
		<div class="field fw2"></div>
		<div class="field fw3">10x buffer</div>
		<div class="field fw3">2 µL</div>
	</div>
	<div class="record">
		<div class="field fw2"></div>
		<div class="field fw3">T4 ligase</div>
		<div class="field fw3">0.5 µL</div>
	</div>
	<div class="record">
		<div class="field fw2"></div>
		<div class="field fw3">\( \mathrm{H_2O}\)</div>
		<div class="field fw3">2.5 µL</div>
	</div>
	<div class="record">
		<div class="field fw2">24 lig</div>
		<div class="field fw3">insert (tasA dig)</div>
		<div class="field fw3">10 µL</div>
	</div>
	<div class="record">
		<div class="field fw2"></div>
		<div class="field fw3">vector(24 dig)</div>
		<div class="field fw3">5 µL</div>
	</div>
	<div class="record">
		<div class="field fw2"></div>
		<div class="field fw3">10x buffer</div>
		<div class="field fw3">2 µL</div>
	</div>
	<div class="record">
		<div class="field fw2"></div>
		<div class="field fw3">T4 ligase</div>
		<div class="field fw3">0.5 µL</div>
	</div>
	<div class="record">
		<div class="field fw2"></div>
		<div class="field fw3">\( \mathrm{H_2O}\)</div>
		<div class="field fw3">2.5 µL</div>
	</div>
	</div>
	<div class="caption">Components of ligation samples.</div>
</div>

<div class="text">The mixtures were ligated for 2 hours at room temperature.</div>
}}