Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/13 August 2015"
Oliviacheng (Talk | contribs) (→08/13/2015) |
Oliviacheng (Talk | contribs) (→PCR amplification of M1-12) |
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==PCR amplification of M1-12== | ==PCR amplification of M1-12== | ||
| − | + | {| class="wikitable" | |
| + | | Q5 buffer | ||
| + | | 10 uL | ||
| + | |- | ||
| + | | dNTPs | ||
| + | | 1 uL | ||
| + | |- | ||
| + | | F-03 | ||
| + | | 2.5 uL | ||
| + | |- | ||
| + | | G-03 | ||
| + | | 2.5 uL | ||
| + | |- | ||
| + | | ddH2O | ||
| + | | 32.5 uL | ||
| + | |- | ||
| + | | Template | ||
| + | | 1 uL | ||
| + | |- | ||
| + | | Q5 | ||
| + | | 0.5 uL | ||
| + | |} | ||
| + | |||
[[File:PCR0813.001.jpg| 800px]] | [[File:PCR0813.001.jpg| 800px]] | ||
Revision as of 03:47, 15 August 2015
08/13/2015
Colony PCR for M1-9(T7) and M1SeqAB1
Same protocol from yesterday
Ran samples on 1.5%
Lane 2-5: M1-9(T7) colony 1-4 Lane 7-10: M1SeqAB1 colony 1-4
PCR amplification of M1-12
| Q5 buffer | 10 uL |
| dNTPs | 1 uL |
| F-03 | 2.5 uL |
| G-03 | 2.5 uL |
| ddH2O | 32.5 uL |
| Template | 1 uL |
| Q5 | 0.5 uL |
