Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/13 August 2015"
Oliviacheng (Talk | contribs) (→Colony PCR for M1-9(T7) and M1SeqAB1) |
Oliviacheng (Talk | contribs) (→Colony PCR for M1-9(T7) and M1SeqAB1) |
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All samples showed bands of expected size | All samples showed bands of expected size | ||
| + | M1-9(T7) Colony 3 has a slight smaller band than the others | ||
==PCR amplification of M1-12== | ==PCR amplification of M1-12== | ||
Revision as of 22:39, 15 August 2015
08/13/2015
Colony PCR for M1-9(T7) and M1SeqAB1
Same protocol from yesterday
Ran samples on 1.5%
All samples showed bands of expected size M1-9(T7) Colony 3 has a slight smaller band than the others
PCR amplification of M1-12
| Q5 buffer | 10 uL |
| dNTPs | 1 uL |
| F-03 | 2.5 uL |
| G-03 | 2.5 uL |
| ddH2O | 32.5 uL |
| Template | 1 uL |
| Q5 | 0.5 uL |
