Difference between revisions of "Team:Technion Israel/Lab Notebook/Overview"
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Revision as of 08:16, 16 August 2015
![Team Logo](https://static.igem.org/mediawiki/2015/f/fc/Team_Technion_Logo.png)
Lab Notebook Overview
Week 1: May 2-8
Bacillus
content
E.coli
Planned the Gibson reaction for our gBlock of 3α-HSD and the plasmid N155 (from our mentors) containing pT7, RBS and mcherry.
Ordered the gBlock of 3α-HSD, which was optimized for E. coli codon usage. We also ordered primers for the Gibson reaction for the gBlock.
Performed reverse PCR to the N155 plasmid and carried out a DPN1 reaction on the product.
Active team members this week: Adi and Alexey
Modeling
content
Comb
content
NADPH
content
Week 2: May 9-15
Bacillus
content
E.coli
Our gBlock arrived!!!
We performed PCR on the 3αHSD gBlock with only 10 cycles (as recommended), but received no band in the gel
We performed PCR on the 3αHSD gBlock in higher temperatures, but still received no bands in the gel
Active team members this week: Adi
Modeling
content
Comb
content
NADPH
content
Week 3: May 16-22
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 4: May 23-29
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 5: May 30-June 5
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 6: June 6-12
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 7: June 13-19
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 8: June 20-26
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 9: June 27-July 3
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 10: July 4-10
Bacillus
content
E.coli
We took a break for our exams.
Modeling
content
Comb
content
NADPH
content
Week 11: July 11-17
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 12: July 18-24
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 13: July 25-31
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 14: August 1-7
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 15: August 8-14
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 16: August 15-21
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 17: August 22-28
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 18: August 29-September 4
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 19: September 5-11
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content
Week 20: September 12-18
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content