Difference between revisions of "Team:NTU-LIHPAO-Taiwan/Notebook"

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<div class="Container2"><div class="Text2" id="First1">7/7</div></div>
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<Red>Learn how to extract the plasmid</Red>
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<Red>Learn how to conduct the gel extraction and electrophoresis</Red>
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<Green>Apoptosis part:</Green>
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Extract the plasmid:
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[RBS](BBa_B0034), [C2](BBa_C0053)
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Restriction enzyme:
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[RBS] cleave by EcoRI, [C2] cleave by EcoRI and PstI
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Gel electrophoresis:
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[RBS] ----- Result: Failed (Smeared band was too blurred)
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[C2] ----- Result: Succeeded
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Transformation of:
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[pC1](BBa_R0051),[pC2](BBa_R0053),[endolysin](BBa_K112806) <b>again</b>
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Revision as of 03:48, 17 August 2015

NTU-LIHPAO-Taiwan

July
7/4
Manufacture CP+ plate
7/6
Learn how to transform
Apoptosis part:
Transformation of:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
7/7
Learn how to manufacture competent cell
Manufacture competent cell
PYY part:
Transformation of:
[promoter](BBa_J23100), [signal peptide](BBa_K554002), [terminator](BBa_B0015)
Apoptosis part:
Transformation of:
[pC1](BBa_R0051), [pC2](BBa_R0053), [endolysin](BBa_K112806), [C1](BBa_C0051)
7/7
Learn how to extract the plasmid
Learn how to conduct the gel extraction and electrophoresis
Apoptosis part:
Extract the plasmid:
[RBS](BBa_B0034), [C2](BBa_C0053)
Restriction enzyme:
[RBS] cleave by EcoRI, [C2] cleave by EcoRI and PstI
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C2] ----- Result: Succeeded
Transformation of:
[pC1](BBa_R0051),[pC2](BBa_R0053),[endolysin](BBa_K112806) again