Difference between revisions of "Team:NTU-LIHPAO-Taiwan/Notebook"

Line 617: Line 617:
 
</div>
 
</div>
 
<div class="Text3_SubSub">
 
<div class="Text3_SubSub">
−
[promoter](BBa_J23100), [signal peptide](BBa_K554002), [terminator](BBa_B0015)
+
[promoter] (BBa_J23100), [signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
 
</div>
 
</div>
 
<div class="Text3">
 
<div class="Text3">
Line 626: Line 626:
 
</div>
 
</div>
 
<div class="Text3_SubSub">
 
<div class="Text3_SubSub">
−
[pC1](BBa_R0051), [pC2](BBa_R0053), [endolysin](BBa_K112806), [C1](BBa_C0051)
+
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806), [C1] (BBa_C0051)
 
</div>
 
</div>
  
−
<div class="Container2"><div class="Text2" id="First1">7/7</div></div>
+
<div class="Container2"><div class="Text2" id="First1">7/8</div></div>
 
<div class="Text3">
 
<div class="Text3">
 
<Red>Learn how to extract the plasmid</Red>
 
<Red>Learn how to extract the plasmid</Red>
Line 643: Line 643:
 
</div>
 
</div>
 
<div class="Text3_SubSub">
 
<div class="Text3_SubSub">
−
[RBS](BBa_B0034), [C2](BBa_C0053)
+
[RBS] (BBa_B0034), [C2] (BBa_C0053)
 
</div>
 
</div>
 
<div class="Text3_Sub">
 
<div class="Text3_Sub">
Line 664: Line 664:
 
</div>
 
</div>
 
<div class="Text3_SubSub">
 
<div class="Text3_SubSub">
−
[pC1](BBa_R0051), [pC2](BBa_R0053), [endolysin](BBa_K112806) <b>again</b>
+
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806) <b>again</b>
 
</div>
 
</div>
 +
 +
<div class="Container2"><div class="Text2" id="First1">7/9</div></div>
 +
<div class="Text3">
 +
<Green>Apoptosis part:</Green>
 +
</div>
 +
<div class="Text3_Sub">
 +
Extract the plasmid:
 +
</div>
 +
<div class="Text3_SubSub">
 +
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
 +
</div>
 +
<div class="Text3_Sub">
 +
Gel electrophoresis:
 +
</div>
 +
<div class="Text3_SubSub">
 +
[RBS] ----- Result: Failed (Smeared band was too blurred)
 +
</div>
 +
<div class="Text3_SubSub">
 +
[C1] ----- Result: Succeeded
 +
</div>
 +
<div class="Text3_SubSub">
 +
[endolysin] ----- Result: Failed (Smeared band was too blurred)
 +
</div>
 +
 +
 +
 +
 +
  
  

Revision as of 03:55, 17 August 2015

NTU-LIHPAO-Taiwan

July
7/4
Manufacture CP+ plate
7/6
Learn how to transform
Apoptosis part:
Transformation of:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
7/7
Learn how to manufacture competent cell
Manufacture competent cell
PYY part:
Transformation of:
[promoter] (BBa_J23100), [signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
Apoptosis part:
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806), [C1] (BBa_C0051)
7/8
Learn how to extract the plasmid
Learn how to conduct the gel extraction and electrophoresis
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
Restriction enzyme:
[RBS] cleave by EcoRI, [C2] cleave by EcoRI and PstI
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C2] ----- Result: Succeeded
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806) again
7/9
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C1] ----- Result: Succeeded
[endolysin] ----- Result: Failed (Smeared band was too blurred)