Difference between revisions of "Team:NTU-LIHPAO-Taiwan/Notebook"

Line 790: Line 790:
 
[promoter] ------ Result: Failed (white colonies didn’t have band, red colonies had band but not the correct size, we speculated that was pollution)
 
[promoter] ------ Result: Failed (white colonies didn’t have band, red colonies had band but not the correct size, we speculated that was pollution)
  
 +
</div>
 +
 +
 +
<div class="Container2"><div class="Text2" id="First1">7/20</div></div>
 +
<div class="Text3">
 +
<Red>Discuss about total synthesis</Red>
 +
</div>
 +
 +
 +
<div class="Container2"><div class="Text2" id="First1">7/13</div></div>
 +
<div class="Text3">
 +
<Green>PYY part:</Green>
 +
</div>
 +
<div class="Text3_Sub">
 +
Transformation of:
 +
</div>
 +
<div class="Text3_SubSub">
 +
[terminator] (BBa_B0015)
 +
</div>
 +
 +
 +
<div class="Container2"><div class="Text2" id="First1">7/24</div></div>
 +
<div class="Text3">
 +
<Red>Discuss our project with professor</Red>
 +
</div>
 +
<div class="Text3">
 +
<Green>PYY part:</Green>
 +
</div>
 +
<div class="Text3_Sub">
 +
Extract the plasmid:
 +
</div>
 +
<div class="Text3_SubSub">
 +
[terminator] (BBa_B0015)
 +
</div>
 +
<div class="Text3_Sub">
 +
Gel electrophoresis:
 +
</div>
 +
<div class="Text3_SubSub">
 +
[terminator] ----- Result: Failed
 +
</div>
 +
 +
 +
<div class="Container2"><div class="Text2" id="First1">7/25</div></div>
 +
<div class="Text3">
 +
<Red>Discuss our problems with Instructor</Red>
 +
</div>
 +
 +
 +
<div class="Container2"><div class="Text2" id="First1">7/26</div></div>
 +
<div class="Text3">
 +
<Green>PYY part:</Green>
 +
</div>
 +
<div class="Text3_SubSub">
 +
Cultured [terminator] again
 +
</div>
 +
 +
 +
<div class="Container2"><div class="Text2" id="First1">7/27</div></div>
 +
<div class="Text3">
 +
<Green>PYY part:</Green>
 +
</div>
 +
<div class="Text3_Sub">
 +
Extract the plasmid:
 +
</div>
 +
<div class="Text3_SubSub">
 +
[terminator] (BBa_B0015)
 +
</div>
 +
<div class="Text3_Sub">
 +
Gel electrophoresis:
 +
</div>
 +
<div class="Text3_SubSub">
 +
[terminator] ----- Result: Succeeded
 
</div>
 
</div>
  

Revision as of 06:45, 17 August 2015

NTU-LIHPAO-Taiwan

July
7/4
Manufacture CP+ plate
7/6
Learn how to transform
Apoptosis part:
Transformation of:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
7/7
Learn how to manufacture competent cell
Manufacture competent cell
PYY part:
Transformation of:
[promoter] (BBa_J23100), [signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
Apoptosis part:
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806), [C1] (BBa_C0051)
7/8
Learn how to extract the plasmid
Learn how to conduct the gel extraction and electrophoresis
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
Restriction enzyme:
[RBS] cleave by EcoRI, [C2] cleave by EcoRI and PstI
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C2] ----- Result: Succeeded
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806) again
7/9
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C1] ----- Result: Succeeded
[endolysin] ----- Result: Failed (Smeared band was too blurred)
PYY part:
Extract the plasmid:
[signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
Gel electrophoresis:
[signal peptide] ----- Result: Succeeded
[terminator] ----- Result: Succeeded
7/10
Manufacture CP+ plate
Manufacture Amp+ plate
Apoptosis part:
Gel electrophoresis:
[RBS] (BBa_B0034) ----- Result: Failed (Smeared band was too blurred)
[C2] (BBa_C0053) ----- Result: Succeeded
Gel extraction:
[C2]
7/11
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
PYY part:
Transformation of:
[promoter] (BBa_J23101)
7/12
Apoptosis part:
Extract the plasmid:
[pC1] (BBa_R0051)
7/13
PYY part:
Extract the plasmid:
[promoter] (BBa_J23101)
Gel electrophoresis (two white colonies & two red colonies):
[promoter] ------ Result: Failed (white colonies didn’t have band, red colonies had band but not the correct size, we speculated that was pollution)
7/20
Discuss about total synthesis
7/13
PYY part:
Transformation of:
[terminator] (BBa_B0015)
7/24
Discuss our project with professor
PYY part:
Extract the plasmid:
[terminator] (BBa_B0015)
Gel electrophoresis:
[terminator] ----- Result: Failed
7/25
Discuss our problems with Instructor
7/26
PYY part:
Cultured [terminator] again
7/27
PYY part:
Extract the plasmid:
[terminator] (BBa_B0015)
Gel electrophoresis:
[terminator] ----- Result: Succeeded