Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/13 August 2015"

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=08/13/2015=
 
=08/13/2015=
  
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==Colony PCR for M1-9(T7) and M1SeqAB1==
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==Colony PCR for M1-9(T7) and M1SeqAB2==
  
 
Same protocol from  [https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/12_August_2015 yesterday]
 
Same protocol from  [https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/12_August_2015 yesterday]
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Ran samples on 1.5%
 
Ran samples on 1.5%
  
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Lane 2-5: M1-9(T7) colony 1-4
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[[ File:2015-08-13 17hr 01min.jpg |500px]]
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Lane 7-10: M1SeqAB1 colony 1-4
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 +
All samples showed bands of expected size
 +
M1-9(T7) Colony 3 has a slight smaller band than the others
 +
M1-Seq1AB is actually M1-Seq2AB
 +
 
 +
==PCR amplification of M1-12==
 +
 
 +
{| class="wikitable"
 +
| Q5 buffer
 +
| 10 uL
 +
|-
 +
| dNTPs
 +
| 1 uL
 +
|-
 +
| F-03
 +
| 2.5 uL
 +
|-
 +
| G-03
 +
| 2.5 uL
 +
|-
 +
| ddH2O
 +
| 32.5 uL
 +
|-
 +
| Template
 +
| 1 uL
 +
|-
 +
| Q5
 +
| 0.5 uL
 +
|}
 +
 
 +
 
 +
[[File:PCR0813.001.jpg| 800px]]

Latest revision as of 17:53, 17 August 2015

iGEM UCLA




08/13/2015

Colony PCR for M1-9(T7) and M1SeqAB2

Same protocol from yesterday

Ran samples on 1.5%

2015-08-13 17hr 01min.jpg

All samples showed bands of expected size M1-9(T7) Colony 3 has a slight smaller band than the others M1-Seq1AB is actually M1-Seq2AB

PCR amplification of M1-12

Q5 buffer 10 uL
dNTPs 1 uL
F-03 2.5 uL
G-03 2.5 uL
ddH2O 32.5 uL
Template 1 uL
Q5 0.5 uL


PCR0813.001.jpg