Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/13 August 2015"
Oliviacheng (Talk | contribs) (→Colony PCR for M1-9(T7) and M1SeqAB1) |
Vinsonclam (Talk | contribs) (→Colony PCR for M1-9(T7) and M1SeqAB2) |
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=08/13/2015= | =08/13/2015= | ||
| − | ==Colony PCR for M1-9(T7) and | + | ==Colony PCR for M1-9(T7) and M1SeqAB2== |
Same protocol from [https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/12_August_2015 yesterday] | Same protocol from [https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/12_August_2015 yesterday] | ||
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All samples showed bands of expected size | All samples showed bands of expected size | ||
| + | M1-9(T7) Colony 3 has a slight smaller band than the others | ||
| + | M1-Seq1AB is actually M1-Seq2AB | ||
==PCR amplification of M1-12== | ==PCR amplification of M1-12== | ||
Latest revision as of 17:53, 17 August 2015
08/13/2015
Colony PCR for M1-9(T7) and M1SeqAB2
Same protocol from yesterday
Ran samples on 1.5%
All samples showed bands of expected size M1-9(T7) Colony 3 has a slight smaller band than the others M1-Seq1AB is actually M1-Seq2AB
PCR amplification of M1-12
| Q5 buffer | 10 uL |
| dNTPs | 1 uL |
| F-03 | 2.5 uL |
| G-03 | 2.5 uL |
| ddH2O | 32.5 uL |
| Template | 1 uL |
| Q5 | 0.5 uL |
