Difference between revisions of "Team:Warwick/Modelling1"
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<h5 class="sidebartitle">DNA Origami</h5> | <h5 class="sidebartitle">DNA Origami</h5> | ||
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| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/7/7d/Warwick3armedglue.png" class="pics" alt=""> This shows how the DNA strands come together. Three double stranded strings of DNA are denatured and then when slowly cooled will come together to form the Y shape. However after the denaturing each strand of DNA has an equal chance of bonding to the original piece of DNA as it does to the correct origami side. Therefore the more complex the structure the less likely it is that that structure will fully form. |
<p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/4/44/WarwickY_PlasmidSequences.png" height="300px" width="300px" border="1px"></p>This is a sequence we came up with for a Y shaped origami structure. <br><br> | <p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/4/44/WarwickY_PlasmidSequences.png" height="300px" width="300px" border="1px"></p>This is a sequence we came up with for a Y shaped origami structure. <br><br> | ||
<br>The highlighted colours correspond to half of one arm which is complementary to half of another arm of the same colour. At the ends of each coloured string is the binding site for a zinc finger.<br> | <br>The highlighted colours correspond to half of one arm which is complementary to half of another arm of the same colour. At the ends of each coloured string is the binding site for a zinc finger.<br> | ||
Revision as of 10:31, 19 August 2015
This shows how the DNA strands come together. Three double stranded strings of DNA are denatured and then when slowly cooled will come together to form the Y shape. However after the denaturing each strand of DNA has an equal chance of bonding to the original piece of DNA as it does to the correct origami side. Therefore the more complex the structure the less likely it is that that structure will fully form.
It is paramount that the length of the plasmid arms are kept to a minimum length as the longer the arms the more unstable the resulting structure will be. It would also take a longer time to form and would have a lower probability of formation. However if the plasmid arms are kept to the smallest possible size it decreases the likelihood of the correct number of E.coli cells bonding to the ends (we have assumed that the ends of the E.coli are perfect spheres and will bond in the centre- if this is not the case the you will need an extra length to accommodate. We calculated 30% would be the optimum error margin to add). 
