Difference between revisions of "Team:Warwick/Modelling1"
| Line 49: | Line 49: | ||
<br><b>Procedure</b> | <br><b>Procedure</b> | ||
<br>1) Remove insert from plasmid | <br>1) Remove insert from plasmid | ||
| − | <br>2) Use restriction enzymes to cut the plasmids into smaller pieces of equal length (150 bp) | + | <br>2) PCR the plasmids |
| − | <br> | + | <br>3) Use restriction enzymes to cut the plasmids into smaller pieces of equal length (150 bp) |
| − | <br> | + | <br>4) Create "linker" DNA |
| − | <br> | + | <br>5) Denature the double stranded DNA and add linkers |
| + | <br>6) Allow to cool and be amazed at the results | ||
<p style="float: right;"> | <p style="float: right;"> | ||
Revision as of 11:04, 19 August 2015
