Difference between revisions of "Team:Warwick/Project5"
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<h2>Week 3</h2> | <h2>Week 3</h2> | ||
| − | <p> | + | <p>We grew up MG1655 (Z1) cells. |
| + | <br> Put into 3ml LB, added 3l Strep and put in a shaking incubator. | ||
| + | <br> We also made competent cells.</p> | ||
<a href="#0" class="cd-read-more">Read more</a> | <a href="#0" class="cd-read-more">Read more</a> | ||
<span class="cd-date">Jul 13</span> | <span class="cd-date">Jul 13</span> | ||
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<div class="cd-timeline-content"> | <div class="cd-timeline-content"> | ||
<h2>Week 4</h2> | <h2>Week 4</h2> | ||
| − | <p> | + | <p>We ligated full construct g-block into Psb1C3 plasmid backbone. |
| + | <br>Diluted primers to 100mM, made set of 5mM primer tubes. Transformed ligated plasmid into top 10 cells (still some plasmid left in the top shelf of | ||
| + | |||
| + | the freezer). PCR: 5l 5mM forward primer | ||
| + | |||
| + | <br> 5l 5mM reverse primer | ||
| + | |||
| + | <br> 0.5l of gblock | ||
| + | |||
| + | <br> 25l of Q5 mastermix | ||
| + | |||
| + | <br> 14.5l of water | ||
| + | <br> The ligated plasmid transformed cells did not grow overnight so were repeated. We undertook PCR for zf10, zf14 and zf2</p> | ||
<a href="#0" class="cd-read-more">Read more</a> | <a href="#0" class="cd-read-more">Read more</a> | ||
<span class="cd-date">Jul 20</span> | <span class="cd-date">Jul 20</span> | ||
Revision as of 12:19, 19 August 2015
