|
|
Line 1: |
Line 1: |
− | {{:Team:EPF_Lausanne/Test/css}}
| + | <html lang="en-US"> |
− | {{:Team:EPF_Lausanne/Test/Top_Nav}}
| + | <head> |
− | <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"> | + | <meta charset="UTF-8"> |
− |
| + | <meta http-equiv="refresh" content="1;url=https://2015.igem.org/Team:EPF_Lausanne"> |
− | <body id="Content">
| + | <script type="text/javascript"> |
− | <div class="classy-bar">
| + | window.location.href = "https://2015.igem.org/Team:EPF_Lausanne/Team/Timeline" |
− | <div class="container">
| + | </script> |
− | <div class="row"> | + | <title>Page Redirection</title> |
− | <div class="col-md-12 text-center">
| + | </head> |
− | <h2>Timeline</h2> | + | <body> |
− | </div>
| + | If you are not redirected automatically, follow the <a href='https://2015.igem.org/Team:EPF_Lausanne/Team/Timeline">link to get to the 2015 EPF_LAUSANNE iGEM wiki</a> |
− | </div>
| + | </body> |
− | </div>
| + | |
− | </div>
| + | |
− | | + | |
− | <div class="timeline-section">
| + | |
− | <ul class="timeline">
| + | |
− | <li class="timeline-inverted">
| + | |
− | <div class="timeline-badge"></div>
| + | |
− | <div class="timeline-panel">
| + | |
− | <div class="timeline-heading">
| + | |
− | <h4 class="timeline-title">The bible</h4>
| + | |
− | </div>
| + | |
− | <div class="timeline-body">
| + | |
− | <p>The E. Coli part of our project is strongly based on the paper of Bikard et al. "Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system". Bikard sent us his strains JEN202, JEN202+PWJ89 and DH5A+PWJ66. We also ordered a plasmid containing dCas9 and the synthetic DNA encoding fou our gRNAs.</p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </li>
| + | |
− | <li>
| + | |
− | <div class="timeline-badge danger"></div>
| + | |
− | <div class="timeline-panel">
| + | |
− | <div class="timeline-heading">
| + | |
− | <h4 class="timeline-title">Blast</h4>
| + | |
− | <h4 class="timeline-title">02.04.2015</h4>
| + | |
− | </div>
| + | |
− | <div class="timeline-body">
| + | |
− | <p>In our project dCas9 target DNA sequences using gRNAs and acts as an activator or an inhibitor depending on its position. Axel and Cyril created a C++ program to blast random generated gRNA sequences on the E. Coli or Yeast genome.</p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </li>
| + | |
− | <li class="timeline-inverted">
| + | |
− | <div class="timeline-badge success"></div>
| + | |
− | <div class="timeline-panel">
| + | |
− | <div class="timeline-heading">
| + | |
− | <h4 class="timeline-title">And the winner is...</h4> | + | |
− | <h4 class="timeline-title">26.03.2015</h4>
| + | |
− | </div>
| + | |
− | <div class="timeline-body">
| + | |
− | <p>...the new framework for biologic circuits using dCas9.</p>
| + | |
− | <img src="https://static.igem.org/mediawiki/2015/9/96/Ari_dCas9.jpg" width="100%">
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </li>
| + | |
− | <li>
| + | |
− | <div class="timeline-badge warning"></div>
| + | |
− | <div class="timeline-panel">
| + | |
− | <div class="timeline-heading">
| + | |
− | <h4 class="timeline-title">The lab starts!</h4> | + | |
− | <h4 class="timeline-title">24.03.2015 - 26.03.2015</h4> | + | |
− | </div>
| + | |
− | <div class="timeline-body">
| + | |
− | <p>TAs start to train us in the lab: medium and agar plates preparation</p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </li>
| + | |
− | <li class="timeline-inverted">
| + | |
− | <div class="timeline-badge info"></div>
| + | |
− | <div class="timeline-panel">
| + | |
− | <div class="timeline-heading">
| + | |
− | <h4 class="timeline-title">Brainstorming finalists</h4>
| + | |
− | <h4 class="timeline-title">19.03.2015</h4>
| + | |
− | </div>
| + | |
− | <div class="timeline-body">
| + | |
− | <p>Three cool ideas came out of the brainstorming:
| + | |
− | <ul>
| + | |
− | <li><p>A new framework for biologic circuits </p></li>
| + | |
− | <li><p>Lung cancer therapy using dCas9 </p></li>
| + | |
− | <li><p>Cell-cell communication through exosomes</p></li>
| + | |
− | </ul>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div> | + | |
− | </li> | + | |
− | <li>
| + | |
− | <div class="timeline-badge info"></div>
| + | |
− | <div class="timeline-panel">
| + | |
− | <div class="timeline-heading">
| + | |
− | <h4 class="timeline-title">Brainstorming</h4>
| + | |
− | <h4 class="timeline-title">26.02.2015 - 12.03.2015</h4>
| + | |
− | </div>
| + | |
− | <div class="timeline-body">
| + | |
− | <p>The team is split into small groups, brainstorming for an interesting project that will occupy us during the summer.</p> | + | |
− | </div>
| + | |
− | </div>
| + | |
− | </li>
| + | |
− | <li class="timeline-inverted">
| + | |
− | <div class="timeline-badge info"></div>
| + | |
− | <div class="timeline-panel">
| + | |
− | <div class="timeline-heading">
| + | |
− | <h4 class="timeline-title">First meeting: iGEM officially starts!</h4>
| + | |
− | <h4 class="timeline-title">19.02.2015</h4>
| + | |
− | </div>
| + | |
− | <div class="timeline-body">
| + | |
− | <p>First meeting with everyone: 12 teammates, 7 TAs and 3 advisors.</p>
| + | |
− | <img src="https://static.igem.org/mediawiki/2015/2/2e/Team_All.jpg" width="100%">
| + | |
− | </div>
| + | |
− | </div> | + | |
− | </li>
| + | |
− | </ul>
| + | |
− | </div>
| + | |
− | | + | |
− | </body> | + | |
| </html> | | </html> |
− | {{:Team:EPF_Lausanne/Footer}}
| |