Difference between revisions of "Team:Warwick/Modelling5"

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<p> <img src="https://static.igem.org/mediawiki/2015/1/1b/4_Circles_picture.png" align="left" height="3600px" width="360px" border="1px">
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<br>Solutions for the smallest diameter circles into which ‘n’ unit-diameter circles (taken to have a relative diameter of 1) can be packed are shown in the table above. Considering the shape of the megastructure, and looking at it at any angle as a 2-D shape, it becomes apparent that the most appropriate model to use is the model for 4 circles packed within a larger circle.
  
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<br>The smaller the tetrahedrons the stronger they are and hence the stronger the resulting formed 3D structure will be. However by decreasing their size you increase the amount of DNA you need to construct it which adds complexity, takes more time and is more expensive. Therefore it is important to find a compromise between size and amount of DNA used.
 
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From reading various papers, such as <a href="http://www.hindawi.com/journals/jna/2011/360954/">this one</a> we determined the maximum size you could make was a tetrahedron with side lengths of 75nm. This size maximised stiffness and strength while minimising the amount of DNA.
 
 
<br>This graph shows the exponential increase of the number of tetrahedrons needed to bond various amounts of E.coli cells to the surface of the formed structure. Due to our time period and budget it would seem a lot better to use a smaller value of E.coli cells to be bonded (<50) to minimise the DNA sequencing.
 
<br>This graph shows the exponential increase of the number of tetrahedrons needed to bond various amounts of E.coli cells to the surface of the formed structure. Due to our time period and budget it would seem a lot better to use a smaller value of E.coli cells to be bonded (<50) to minimise the DNA sequencing.
  

Revision as of 13:47, 19 August 2015

Warwick iGEM 2015

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