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− | <div class="container-fluid page-heading">
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− | <h3>Interlab Study</h3>
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− | </div>
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− | <div class="container-fluid content-container">
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− | <div class="col-md-9">
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− | <div class="section" id="Introduction">
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− | <h2>Introduction</h2>
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− | <div class="section" id="Overview">
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− | <h3>Overview</h3>
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− | <p>
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− | One of the fundamental principles of synthetic biology is the characterization of standard biological parts. iGEM HQ's
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− | International InterLab Measurement Study is an endeavour at achieving the large-scale characterization of a set of
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− | biological devices which has been previously designed and catalogued in the BioBricks Registry. In this study, iGEM teams
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− | from across the world set out to measure the fluorescence of model organisms which have been transformed with devices
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− | comprising the same green fluorescent protein (GFP) gene expressed at different levels by being fused to promoters of
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− | different strengths. This would provide a large dataset from which a more accurate analysis that accounts for lab-to-lab
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− | variations can be drawn.
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− | </p>
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− | <p>
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− | The promoters characterized in the 2015 InterLab Study are three constitutive promoters of different strengths found
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− | within the <a href="http://parts.igem.org/Promoters/Catalog/Anderson">Anderson Promoter Collection</a>, which were included
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− | in the 2015 iGEM Distribution Kit. We assembled each of these three promoters upstream of a given GFP-encoding BioBrick and
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− | transformed each of the composite parts into three different strains of E. coli to measure their respective fluorescence levels.
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− | We measured fluorescence levels using a 96-well microplate reader, a flow cytometer, as well as a confocal microscope.
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− | </p>
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− | <div class"section" id="KeyFindings">
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− | <h3>Key Results and Findings</h3>
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− | Through the data obtained from our microplate reader, we found that the strengths of the three promoters investigated are in the same order as Anderson et al's initial characterization
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− | across the three E. coli strains which we studied. The relative fluorescence magnitudes we measured however do not agree with
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− | the ratios presented in the Anderson study.
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− | </p>
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− | <p>
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− | To eliminate the dependence on equipment parameters associated with arbitrary fluorescence units, we calibrated our fluorescence
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− | readings against different concentrations of a fluorescent chemical standard, sodium fluorescein, and expressed fluorescence
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− | intensity in units of its concentration.
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− | </p>
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− | </div>
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− | <div class="col-md-3 contents-sidebar">
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− | <ul id="sidebar" class="nav nav-stacked" data-spy="affix">
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− | <li>
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− | <a href="#Introduction">Introduction</a>
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− | <ul class="nav nav-stacked">
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− | <li><a href="#Overview">Overview</a></li>
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− | <li><a href="#KeyFindings">Key Results and Findings</a></li>
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− | </ul>
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− | </li>
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− | </div>
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− | </div>
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− | </div>
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− | </html>
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