Difference between revisions of "Troubleshooting/Ligation"
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| − | Now that you know you <a href"https://2015.igem.org/Troubleshooting/Transformation">transformation efficiency</a> and it's above 1 x 10<sup>8</sup> CFU/µg DNA, we can work on other possible problems if you're still not getting great results from your cloning. | + | Now that you know you <a href"https://2015.igem.org/Troubleshooting/Transformation">transformation efficiency</a> and it's above 1 x 10<sup>8</sup> CFU/µg DNA, we can work on other possible problems if you're still not getting great results from your cloning. This page is focused on common problems students have with ligations. |
<br><br>Here are some tips to help you troubleshoot this problem: | <br><br>Here are some tips to help you troubleshoot this problem: | ||
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Revision as of 17:32, 7 May 2015
Ligation Troubleshooting
Now that you know you transformation efficiency and it's above 1 x 108 CFU/µg DNA, we can work on other possible problems if you're still not getting great results from your cloning. This page is focused on common problems students have with ligations.
Here are some tips to help you troubleshoot this problem: