Difference between revisions of "Troubleshooting/Ligation"
| Line 36: | Line 36: | ||
<b>No colonies</b> | <b>No colonies</b> | ||
| + | <ol><li><b>Uncut plasmid:</b> It's possible that your destination backbone was not cut well during your digest. </li> | ||
| + | <li><b>Ligase didn't work:</b> | ||
Revision as of 19:37, 7 May 2015
Ligation Troubleshooting
Now that you know you transformation efficiency and it's above 1 x 108 CFU/µg DNA, we can work on other possible problems if you're still not getting great results from your cloning. This page is focused on common problems students have with ligations.
No colonies
- Uncut plasmid: It's possible that your destination backbone was not cut well during your digest.
- Ligase didn't work: