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Revision as of 16:05, 25 August 2015
GOPTS surface
protocol for derivatisation of glass slides with GOPTS
According to protocoll from Günter
material: GOPTS, aqua dest., acetone; (plasma generator, nitrogen gun)
duration: ca. 2h
- Name slides
- Wash with aqua dest.
- Dry with wafergun
- Activate in plasma generator: gas flow: 40-80 L/h, time: 40 s - 1 min
- Pipette 80 µL of GOPTS-solution per glass sandwich
- Incubate for 1 h at RT
- Part the sandwiches and put in acetone for 30 s
- Wash with acetone (work fast!!)
- Dry with wafergun
storable for up to 2 weeks.