|
|
| (31 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| − | {{2015Top}}
| |
| − | <html>
| |
| | | | |
| − |
| |
| − | <!-- this is where the submenu is placed -->
| |
| − |
| |
| − |
| |
| − | <div id="leftColumn">
| |
| − | <h4><a href= "https://2015.igem.org/Troubleshooting">Troubleshooting</a></h4>
| |
| − | <ul>
| |
| − | <a href= "https://2015.igem.org/Troubleshooting#intro"> <li>Introduction</li></a>
| |
| − | <a href= "https://2015.igem.org/Troubleshooting#tips"> <li>General Tips and Tricks</li></a>
| |
| − | <ol><a href= "https://2015.igem.org/Troubleshooting/Transformation"> <li>Transformation</li></a>
| |
| − | <a href="https://2015.igem.org/Troubleshooting/Ligation"> <li>Ligation and Restriction Digest</li></ol>
| |
| − | <a href= "https://2015.igem.org/Troubleshooting#protocols"> <li>iGEM Protocols</li></a>
| |
| − | <a href= "https://2015.igem.org/Troubleshooting#answer"> <li>Answers</li></a>
| |
| − | <a href= "https://2015.igem.org/Troubleshooting#ask"> <li>Ask Your Questions</li></a>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − |
| |
| − | <!-- this is where the content is placed --->
| |
| − | <div id="rightColumn">
| |
| − |
| |
| − | <!-- start of content ---------------------------------------->
| |
| − | <div class="alertMessage"> Page is under construction. <br>iGEM HQ is currently working on updating this information
| |
| − | for the iGEM 2015 competition. </div>
| |
| − |
| |
| − | <h2>Ligation Troubleshooting</h2>
| |
| − |
| |
| − | <p>
| |
| − | Now that you know you <a href"https://2015.igem.org/Troubleshooting/Transformation">transformation efficiency</a> and it's above 1 x 10<sup>8</sup> CFU/µg DNA, we can work on other possible problems if you're still not getting great results from your cloning. This page is focused on common problems students have with ligations.
| |
| − | <br><br>
| |
| − |
| |
| − | <b>No colonies</b>
| |
| − | <p>
| |
| − | If you know your cells are working well, there are a few common ligation problems that might be happening with your reaction.
| |
| − |
| |
| − | <ol><li><b>Uncut insert:</b> It's possible that your insert was not cut well during your digest. </li>
| |
| − | <li><b>Ligase didn't work:</b>
| |
| − |
| |
| − |
| |
| − |
| |
| − |
| |
| − | <div class="clear"></div><div class="clear"></div>
| |
| − |
| |
| − |
| |
| − |
| |
| − | <!-- end of content ---->
| |
| − |
| |
| − | </div>
| |
| − | <div class="clear"></div>
| |
| − | </html> {{2015Bottom}}<html>
| |
| − | </div>
| |
| − | </html>
| |