Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/19 May 2015"

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**I should have taken the OD 600 at this point, but I forgot to.
 
**I should have taken the OD 600 at this point, but I forgot to.
 
*Mass Balanced the tubes and added a water tube, and spun down the cells at 5300 rpm at 4 C for 15 min. to pellet the cells.
 
*Mass Balanced the tubes and added a water tube, and spun down the cells at 5300 rpm at 4 C for 15 min. to pellet the cells.
−
*Decanted the supernatant.
+
*Decanted the supernatant.  
 +
*Weighed the pellets
 +
**In total, the wet weight of all the pellets across all 5 falcon tubes was 2.42 grams, approximately 0.5 grams per 45 ml of culture.
 +
*Froze down the pellets in the falcon tubes at -80C

Revision as of 20:30, 19 May 2015

5/19

Cell Harvesting

  • Cells were grown up in 250 ml of LB medium and induced with IPTG. See 5/18 for details.
  • The Cells were split up into 5 pre weighed 50 ml falcon tubes, with 45 ml of cells in each tube.
    • I should have taken the OD 600 at this point, but I forgot to.
  • Mass Balanced the tubes and added a water tube, and spun down the cells at 5300 rpm at 4 C for 15 min. to pellet the cells.
  • Decanted the supernatant.
  • Weighed the pellets
    • In total, the wet weight of all the pellets across all 5 falcon tubes was 2.42 grams, approximately 0.5 grams per 45 ml of culture.
  • Froze down the pellets in the falcon tubes at -80C