Difference between revisions of "Team:IONIS Paris/Practices"

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<h2> Human Practices </h2>
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<title >IONIS Paris</title>
  
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<p>iGEM teams are unique and leading the field because they "go beyond the lab" to imagine their projects in a social/environmental context, to better understand issues that might influence the design and use of their technologies.</p>
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<p>Teams work with students and advisors from the humanities and social sciences to explore topics concerning ethical, legal, social, economic, safety or security issues related to their work. Consideration of these Human Practices is crucial for building safe and sustainable projects that serve the public interest. </p>
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<p>For more information, please see the <a href="https://2015.igem.org/Practices_Hub">Practices Hub</a>.</p>
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<h4>Note</h4>
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<p>You must fill out this page in order to be considered for all <a href="https://2015.igem.org/Judging/Awards">awards</a> for Human Practices:</p>
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<ul>
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<li>Human Practices silver medal criterion</li>
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<li>Human Practices gold medal criterion</li>
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<li>Best Integrated Human Practices award</li>
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<li>Best Education and Public Engagement award</li>
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</ul>
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<h5>Some Human Practices topic areas </h5>
 
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<ul>
 
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<li>Philosophy</li>
 
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<li>Public Engagement / Dialogue</li>
 
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<li>Education</li>
 
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<li>Product Design</li>
 
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<li>Scale-Up and Deployment Issues</li>
 
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<li>Environmental Impact</li>
 
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<li>Ethics</li>
 
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<li>Safety</li>
 
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<li>Security</li>
 
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<li>Public Policy</li>
 
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<li>Law and Regulation</li>
 
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<li>Risk Assessment</li>
 
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</ul>
 
  
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<h5>What should we write about on this page?</h5>
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<p>On this page, you should write about the Human Practices topics you considered in your project, and document any special activities you did (such as visiting experts, talking to lawmakers, or doing public engagement).</p>
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<h5>Inspiration</h5>
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<p>Read what other teams have done:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:Dundee/policypractice/experts">2014 Dundee </a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Policy_Practices_Overview">2014 UC Davis </a></li>
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<li><a href="https://2013.igem.org/Team:Manchester/HumanPractices">2013 Manchester </a></li>
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<li><a href="https://2013.igem.org/Team:Cornell/outreach">2013 Cornell </a></li>
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<h3>Integrated Human Practices</h3>
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<h1 class="intro wow zoomIn" wow-data-delay="0.4s" wow-data-duration="0.9s"  style="font-family:sans-serif; color:#B00000 " >Human Practices</h1>
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<p>Do you want to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Integrated Human Practices award</a>? Make it easy for the judges to find any wiki content that is relevant to this prize. Highlight this content with a header or separate section.</p>
 
  
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<h3>Education and Public Engagement</h3>
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<p>Do you want to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Education and Public Outreach award</a>? Make it easy for the judges to find any wiki content that is relevant to this prize. Highlight this content with a header or separate section.</p>
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<div id="about"  class="container spacer about">
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<h2 class="text-center wowload fadeInUp" style="color:#ff0000"><big>IN CONSTRUCTION</big></h2>
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<h4><i class="fa fa-ils"style="color:#b22222"></i> Introduction </h4>
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<!--p align="justify">The Bio-console is a project developed to make science accessible for all and design for all.
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In this way the safety of the project is one of the most important part.
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Make sure that the bacteria are not able to be release in nature.
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To be sure that any bacteria are able to leave the game different measure have been taken; a kill-swirch, a killing-liposome and a hermetic microfluidic chip.
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<h3 align="center">Poularization of Biology</h3>
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<h4><i class="fa fa-question"style="color:#b22222"></i>Why do we chose a kill Switch ?</h4>
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<p align="justify"> A kill switch is a <font style="color:#66cdaa"><b>genetically-encoded suicide trigger</b></font>.
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This trigger can be a change in the environment which will make the organism’s life dependent on it.  
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In our project, the change will be the presence or not of light.
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That is very interesting as we could create a game over in our Bio Console, once we trigger the bacteria’s death through light.
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Moreover, our team is  really concerned about<font style="color:#66cdaa"> BioSafety</font>.
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It is important for us to avoid any possibility of escaping from our microfluidic chip.
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The Kill switch would permit to contain our genetically modified bacteria into the chip through lethality.</p>
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<h4><i class="fa fa-times"style="color:#b22222"></i>How we design it </h4>
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<p align="justify">So we aim to construct an efficient kill-switch triggering by light based on the <font style="color:#66cdaa"><b>pDawn & pDusk - Toxin systems</b></font>.
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In this way we will construct two different parts.
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The first one will be composed of pDawn and <font style="color:#66cdaa"><b>holin/endolysin</b>.</font>
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Toxins will be produced after light stimulation and induce lysis.
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The second part will be composed of pDusk and endolysin/holin complex, this will lead to the cell lysis when the bacteria won’t be exposed to light.
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This second part will be more difficult to characterized indeed all steps before characterization of the plasmid as plasmid amplification will have to be under light. </p>
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<h3 align="center">Education and Public Engagement</h3>
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<h4><i class="fa fa-question"style="color:#b22222"></i>Why do we chose a photoactivable liposome ?</h4>
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<p align="justify">In our Bio-Console, the gameover is based on the death of the bacteria and then the lose of fluorescence.
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For this we have to find an efficient system that will kill the fastest our bacteria.  
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In this way we have introduce a<font style="color:#66cdaa"><b> photosensible liposome </b></font>containing toxins.
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The liposome opening trigger by light allows relase of toxins and the fast death of bacteria.
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<h4><i class="fa fa-times"style="color:#b22222"></i>How we aim to design it </h4>
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<p align="justify"> The light-triggerable liposome will be made with photo-polymerizable phospholipid.
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It will contain <font style="color:#66cdaa"><b>toxins</b></font> previously synthesized by resistant engineered bacteria.
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The liposome will be locked up in a bubble with our bacteria.
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In this way when a bright flash will appear, it will activate the liposome opening and release toxins.
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Finally toxins will quickly eliminate our bacteria, which will lose his fluorescence.
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</p>
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<CENTER>
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<div class="col-sm-3 col-xs-6">
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<i class="fa  fa-male fa-5x"></i><h4>Human Practices</h4>
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<i class="fa fa-share-alt  fa-5x"></i><h4>Share</h4>
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<i class="fa fa-graduation-cap fa-5x"></i><h4>Education</h4>
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<i class="fa fa-users  fa-5x"></i><h4>Engagement</h4>
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<img src="https://static.igem.org/mediawiki/2015/3/34/Wii.jpg" alt="img01"/>
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<figcaption>
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<h2>Bio-Console</h2>
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<p>Our reality game using bacteria<br>
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<a href="https://2015.igem.org/Team:IONIS_Paris/Project" >View more</a></p>           
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</figcaption>
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</figure>
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<!--https://static.igem.org/mediawiki/2015/c/c2/Schema1_IONIS.png-->
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<img src="https://static.igem.org/mediawiki/2015/thumb/b/b1/Optogenetics.jpg/800px-Optogenetics.jpg" alt="team"/>
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<figcaption>
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<h2>OPTOGENETICS</h2>
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<p>Our scientific core of the project<br>
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<a href="https://2015.igem.org/Team:IONIS_Paris/Project/Optogenetics" >View more</a></p>           
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</figcaption>
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</figure>
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<img src="https://static.igem.org/mediawiki/2015/1/18/GRID.jpg" alt="team"/>
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<h2>MICROFLUIDICS</h2>
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<p>Our challenge in achieving the Bio-console<br>
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<a href="https://2015.igem.org/Team:IONIS_Paris/Project/Microfluidics" >View more</a></p>           
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</figcaption>
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</figure>
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<h3 class="title">Title</h3>
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<a class="prev">‹</a>
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Revision as of 12:58, 28 August 2015

IONIS Paris

IN CONSTRUCTION

Introduction

Poularization of Biology

Education and Public Engagement

Human Practices

Share

Education

Engagement

img01

Bio-Console

Our reality game using bacteria
View more

team

OPTOGENETICS

Our scientific core of the project
View more

team

MICROFLUIDICS

Our challenge in achieving the Bio-console
View more




IONIS Paris team. 2015 Edition