Difference between revisions of "Team:Bordeaux/Template:AchievementsProtocols"

 
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<p> Our Organisms of Choice is <I>Escherichia coli</I> and <I>Saccharomyces cerevisiae </I>. Listed below are the protocols we used in our lab. </p>
 
<p> Our Organisms of Choice is <I>Escherichia coli</I> and <I>Saccharomyces cerevisiae </I>. Listed below are the protocols we used in our lab. </p>
  
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<h1> Cloning </h1>
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<h5> Cloning </h5>
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<h1> Working with <I>E.coli</I> </h1>
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<h1> Working with <I>S.cerevisiae </I> </h1>
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  <ul>
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      <li><a href="https://static.igem.org/mediawiki/2015/b/b9/PCR_iGEM_Bordeaux_Protocol.pdf" target="_blank"><font size="3" ><B>PCR Methods iGEM Bordeaux</B></font></a></li>
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      <li><a href="https://static.igem.org/mediawiki/2015/a/a3/E.coli_Competent_Cells_v2.pdf" target="_blank"><font size="3" ><B><I>E.coli</I> Competent Cells</B></font></a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/b/ba/Miniprep_Camilla_V2_.pdf"target="_blank"><font size="3" ><B>Plasmid Extraction using Alkaline Lysis Method</B></font></a></li>
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  </ul>
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<h5> Working with <I>E.coli</I> </h5>
  
 
<ul>
 
<ul>
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      <li><a href="https://static.igem.org/mediawiki/2015/b/b9/PCR_iGEM_Bordeaux_Protocol.pdf" target="_blank">PCR Methods iGEM Bordeaux</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2015/6/60/Growing_Medium_of_E.coli_VC.pdf"target="_blank"><font size="3" ><B>Growing Media of <I>E.coli</I></B></font></a> </li>
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      <li><a href="https://static.igem.org/mediawiki/2015/a/a3/E.coli_Competent_Cells_v2.pdf" target="_blank"><I>E.coli</I> Competent Cells</a></li>
+
 
−
      <li><a href="https://static.igem.org/mediawiki/2015/b/ba/Miniprep_Camilla_V2_.pdf"target="_blank">Plasmid Extraction using Alkaline Lysis Method</a></li>
+
<li><a href="https://static.igem.org/mediawiki/2015/0/08/E.coli_Curdlan_Purification_V%2A%2A.pdf"target="_blank"><font size="3" ><B><I>E.coli</I> Curdlan Purification</B></font></a> </li>
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      <li><a href="https://static.igem.org/mediawiki/2015/2/24/E.coli_Curdlan_Purification.pdf"target="_blank"><I>E.coli</I> Curdlan Purification</a></li>
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 +
 
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</ul>
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<h5> Working with <I>S.cerevisiae </I> </h5>
 +
 
 +
  <ul>
 +
 
 +
    <li><a href="https://static.igem.org/mediawiki/2015/5/5a/High_Efficiency_Yeast_Transformation_.pdf"target="_blank"><font size="3" ><B>High Efficiency Yeast Transformation</B></font></a> </li>
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 +
  <li><a href="https://static.igem.org/mediawiki/2015/7/7d/Genomic_DNA_preparation_from_Yeast_.pdf"target="_blank"><font size="3" ><B> Genomic DNA Preparation from Yeast</B></font></a>  </li>
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 +
 
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</ul>
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<h5> Curdlan Methods </h5>
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 +
<ul>
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 +
 
 +
    <li><a href="https://static.igem.org/mediawiki/2015/b/bb/Curdlan_Quantification.pdf"target="_blank"><font size="3" ><B> Curdlan Quantification</B></font></a></li>
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 +
    <li><a href="https://static.igem.org/mediawiki/2015/4/43/Sulfation_of_polysaccharides_by_Sodium_Pyrosulfate_in_Dim%C3%A9thylsulfoxide_VC.pdf"target="_blank"><font size="3" ><B> Sulfation of Curdlan by Sodium Pyrosulfate in Dimethyl Sulfoxide</B></font></a></li>
  
  

Latest revision as of 17:11, 28 August 2015

Protocols

Our Organisms of Choice is Escherichia coli and Saccharomyces cerevisiae . Listed below are the protocols we used in our lab.

Cloning
Working with E.coli
Working with S.cerevisiae
Curdlan Methods