Benzo[a]pyrene

 ✡ Introduction

   (1)Why do we detect benzo[a]pyrene?

   Because when food,such as fried food ,which is heated above 300 degrees

   celcius,food will release benzo[a]pyrene recycled oil usually contain

   benzo[a]pyrene[1].

   (2)Harm?

   Pathway:from skin,breathing in,eating Carcinogenic,environmental

   pollution, Skin irritation,eye irritation[1].

   (3)Taiwanese regulation

[2]

   (4)National regulation

   Europe:The same to Taiwanese regulation[2]

   American:The MCL has been set at 0.2 ppb[3]

 ✡ Circuit Design

   So far,we don’t find the protein which can combine with the Benzo[a]pyrene,so first

   we must degradate it by Laccase. And its product is Bap-1,6-quinone which can

   combine with the protein,in order that we can detect Benzo[a]pyrene. [4]

   We put the gene fragment which can produce the Laccase in the E.coli

   Let it produce itself.

   QsrR is a protein which can combine with Bap-1,6-quinone.[5]As Laccase,we put the

   gene fragment in the E.coli.Let it produce itself.

   We design a gene part make QsrR repress sfRFP’s production.

   Usually,QsrR is on the Binding Site,and red fluorescent protein will not be produced.

   When QsrR combine with Bap-1,6-quinone, QsrR will be activated and go away.

   And the transcription will carry on,then it will produce red fluorescent protein

 ✡ Result

 ✡ Reference

   (註1)Metabolism of mercury, administered as methylmercuric chloride or mercuric chloride, by lactating ruminants.

   (註2)台北醫學大學公共衛生學系碩士班碩士論文Graduate Institute.

   (註3)Brown, N. L., J. V. Stoyanov, et al. (2003). "The MerR family of transcriptional regulators." FEMS Microbiol Rev 27(2-3): 145-163.

   (註4)Park, S. J., J. Wireman, et al. (1992). "Genetic analysis of the Tn21 mer operator-promoter." J Bacteriol 174(7): 2160-2171.