Difference between revisions of "Team:NTU-LIHPAO-Taiwan/Notebook"

Line 113: Line 113:
 
background-repeat: no-repeat;
 
background-repeat: no-repeat;
 
}
 
}
 +
 +
#Wrapper_Bottom {
 +
width: 100%;
 +
height: 100px;
 +
bottom: 0px;
 +
top: auto;
 +
background-color: #FFF;
 +
border-top-style: solid;
 +
border-top-color: #079235;
 +
border-top-width: 3px;
 +
margin-right: auto;
 +
margin-left: auto;
 +
margin-top: 0px;
 +
margin-bottom: 0px;
 +
z-index: 9998;
 +
position: absolute;
 +
}
 +
 +
  
 
/*-------------------------------------------*/
 
/*-------------------------------------------*/
Line 161: Line 180:
 
}
 
}
  
 +
 +
 +
/*-------------------------------------------*/
 +
/*---------------Bottom icons----------------*/
 +
/*-------------------------------------------*/
 +
 +
#NTUschool_logo {
 +
height: 70px;
 +
width: 265px;
 +
position: absolute;
 +
left: 25%;
 +
top: 5px;
 +
right: auto;
 +
bottom: auto;
 +
z-index: 9999;
 +
margin-left: -132px;
 +
}
 +
 +
#LIHPAO_logo {
 +
height: 70px;
 +
width: 215px;
 +
position: absolute;
 +
left: 75%;
 +
top: 5px;
 +
right: auto;
 +
bottom: auto;
 +
z-index: 9999;
 +
margin-left: -107px;
 +
}
 +
 +
#NTUBST_logo {
 +
height: 70px;
 +
width: 70px;
 +
position: absolute;
 +
left: 50%;
 +
top: 5px;
 +
right: auto;
 +
bottom: auto;
 +
z-index: 9999;
 +
margin-left: -35px;
 +
}
 +
 +
#Bottom_Text {
 +
color: #767676;
 +
bottom: 0px;
 +
width: 100%;
 +
height: auto;
 +
font-size: 15px;
 +
position: absolute;
 +
text-align: center;
 +
}
  
  
Line 909: Line 979:
 
});
 
});
 
</script>
 
</script>
 +
 +
<div id="Wrapper_Bottom">
 +
<div id="NTUschool_logo"><a href="http://www.ntu.edu.tw/english/index.html"><img src="https://static.igem.org/mediawiki/2015/d/de/NTUschool_icon.png" height="70" /></a></div>
 +
<div id="LIHPAO_logo"><a href="http://www.llsc.com.tw/"><img src="https://static.igem.org/mediawiki/2015/d/d4/LIHPAO_logo.png" height="70" /></a></div>
 +
<div id="NTUBST_logo"><a href="http://www.bst.ntu.edu.tw/BST-new/NTUBST.html"><img src="https://static.igem.org/mediawiki/2015/6/68/NTUBST_logo.png" height="70" /></a></div>
 +
<div id="Bottom_Text">
 +
Maintained by the iGEM team NTU-LIHPAO-Taiwan&nbsp;&nbsp;&nbsp;&nbsp;©2015 NTU-LIHPAO-Taiwan
 +
</div>
 +
</div>
 
</div>
 
</div>
  
 
</body>
 
</body>
 
</html>
 
</html>

Revision as of 08:13, 30 August 2015

NTU-LIHPAO-Taiwan

July
7/4
Manufacture CP+ plate
7/6
Learn how to transform
Apoptosis part:
Transformation of:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
7/7
Learn how to manufacture competent cell
Manufacture competent cell
PYY part:
Transformation of:
[promoter] (BBa_J23100), [signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
Apoptosis part:
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806), [C1] (BBa_C0051)
7/8
Learn how to extract the plasmid
Learn how to conduct the gel extraction and electrophoresis
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
Restriction enzyme:
[RBS] cleave by EcoRI, [C2] cleave by EcoRI and PstI
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C2] ----- Result: Succeeded
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806) again
7/9
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C1] ----- Result: Succeeded
[endolysin] ----- Result: Failed (Smeared band was too blurred)
PYY part:
Extract the plasmid:
[signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
Gel electrophoresis:
[signal peptide] ----- Result: Succeeded
[terminator] ----- Result: Succeeded
7/10
Manufacture CP+ plate
Manufacture Amp+ plate
Apoptosis part:
Gel electrophoresis:
[RBS] (BBa_B0034) ----- Result: Failed (Smeared band was too blurred)
[C2] (BBa_C0053) ----- Result: Succeeded
Gel extraction:
[C2]
7/11
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
PYY part:
Transformation of:
[promoter] (BBa_J23101)
7/12
Apoptosis part:
Extract the plasmid:
[pC1] (BBa_R0051)
7/13
PYY part:
Extract the plasmid:
[promoter] (BBa_J23101)
Gel electrophoresis (two white colonies & two red colonies):
[promoter] ------ Result: Failed (white colonies didn’t have band, red colonies had band but not the correct size, we speculated that was pollution)
7/20
Discuss about total synthesis
7/13
PYY part:
Transformation of:
[terminator] (BBa_B0015)
7/24
Discuss our project with professor
PYY part:
Extract the plasmid:
[terminator] (BBa_B0015)
Gel electrophoresis:
[terminator] ----- Result: Failed
7/25
Discuss our problems with Instructor
7/26
PYY part:
Cultured [terminator] again
7/27
PYY part:
Extract the plasmid:
[terminator] (BBa_B0015)
Gel electrophoresis:
[terminator] ----- Result: Succeeded
Maintained by the iGEM team NTU-LIHPAO-Taiwan    ©2015 NTU-LIHPAO-Taiwan