Difference between revisions of "Team:NTU-LIHPAO-Taiwan/Notebook"
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<li><div id=width_small><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan">Home</a></div> | <li><div id=width_small><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan">Home</a></div> | ||
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<li><div id=width_small><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Team">Team</a></div> | <li><div id=width_small><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Team">Team</a></div> | ||
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<li><div id=width_small span style="cursor:default"><a>Project</a></div> | <li><div id=width_small span style="cursor:default"><a>Project</a></div> | ||
<ul class="subs"> | <ul class="subs"> | ||
| − | <li><a href=" | + | <li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Project/Abstract">Abstract</a></li> |
| − | <li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Overview">Overview</a></li> | + | <li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Project/Overview">Overview</a></li> |
| − | <li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Parts">Parts</a></li> | + | <li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Project/Parts">Parts</a></li> |
| − | <li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Results">Results</a></li> | + | <li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Project/Results">Results</a></li> |
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<li><div id=width_small span style="cursor:default"><a>Modeling</a></div> | <li><div id=width_small span style="cursor:default"><a>Modeling</a></div> | ||
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<li><div id=width_small span style="cursor:default"><div id=Position_Now><a>Notebook</a></div></div> | <li><div id=width_small span style="cursor:default"><div id=Position_Now><a>Notebook</a></div></div> | ||
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<li><div id=width_small><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Safety">Safety</a></div> | <li><div id=width_small><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Safety">Safety</a></div> | ||
</li> | </li> | ||
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<li><div id=width_large><a href="#">Human Practice</a></div> | <li><div id=width_large><a href="#">Human Practice</a></div> | ||
<ul class="subs"> | <ul class="subs"> | ||
Revision as of 08:18, 30 August 2015
July
7/4
7/6
Transformation of:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
7/7
Transformation of:
[promoter] (BBa_J23100), [signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806), [C1] (BBa_C0051)
7/8
Extract the plasmid:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
Restriction enzyme:
[RBS] cleave by EcoRI, [C2] cleave by EcoRI and PstI
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C2] ----- Result: Succeeded
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806) again
7/9
Extract the plasmid:
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C1] ----- Result: Succeeded
[endolysin] ----- Result: Failed (Smeared band was too blurred)
Extract the plasmid:
[signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
Gel electrophoresis:
[signal peptide] ----- Result: Succeeded
[terminator] ----- Result: Succeeded
7/10
Gel electrophoresis:
[RBS] (BBa_B0034) ----- Result: Failed (Smeared band was too blurred)
[C2] (BBa_C0053) ----- Result: Succeeded
Gel extraction:
[C2]
7/11
Extract the plasmid:
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
Transformation of:
[promoter] (BBa_J23101)
7/12
Extract the plasmid:
[pC1] (BBa_R0051)
7/13
Extract the plasmid:
[promoter] (BBa_J23101)
Gel electrophoresis (two white colonies & two red colonies):
[promoter] ------ Result: Failed (white colonies didn’t have band, red colonies had band but not the correct size, we speculated that was pollution)
7/20
7/13
Transformation of:
[terminator] (BBa_B0015)
7/24
Extract the plasmid:
[terminator] (BBa_B0015)
Gel electrophoresis:
[terminator] ----- Result: Failed
7/25
7/26
Cultured [terminator] again
7/27
Extract the plasmid:
[terminator] (BBa_B0015)
Gel electrophoresis:
[terminator] ----- Result: Succeeded
Maintained by the iGEM team NTU-LIHPAO-Taiwan ©2015 NTU-LIHPAO-Taiwan