Difference between revisions of "Team:York/Protocols/Growth Media"
Lizalexianu (Talk | contribs) (Created page with "<html> <h2>Lysogeny Broth</h2> <h3>Materials</h3> <ul> <li>10g of tryptone</li> <li>5g of yeast extract</li> <li>10g of NaCl</li> <li>1L of Deionized Water</li> <li> 1M NaCl </l...") |
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<h2>Lysogeny Broth</h2> | <h2>Lysogeny Broth</h2> | ||
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</ul> | </ul> | ||
− | < | + | <h4>Procedure</h4> |
<ol> | <ol> | ||
<li>Use a container with at least double the volume of the liquid that you are making.</li> | <li>Use a container with at least double the volume of the liquid that you are making.</li> | ||
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<li>Autoclave. </li> | <li>Autoclave. </li> | ||
<li>Store at room temperature or +4°C.</li> | <li>Store at room temperature or +4°C.</li> | ||
+ | </ol> | ||
+ | |||
+ | <h2>LB Agar</h2> | ||
+ | |||
+ | <h4>Procedure</h4> | ||
+ | <ol> | ||
+ | <li>follow steps to make lysogeny broth as above</li> | ||
+ | <li>add 15g of agar powder per litre of LB</li> | ||
+ | <li>autoclave in 200 or 250 mL aliquots in 500mL duran bottles</li> | ||
+ | <li>add antibiotic desired to melted agar (~55°C)</li> | ||
+ | <li>pour into petri dishes and leave to harden in asceptic fume hood</li> | ||
+ | <li>invert, label, wrap with parafilm and store at 4°C</li> | ||
</ol> | </ol> | ||
</html> | </html> |
Latest revision as of 15:08, 31 August 2015
Lysogeny Broth
Materials
- 10g of tryptone
- 5g of yeast extract
- 10g of NaCl
- 1L of Deionized Water
- 1M NaCl
- 1M KOH
Procedure
- Use a container with at least double the volume of the liquid that you are making.
- Dissolve 10 g tryptone, 5 g yeast extract, and 10 g NaCl in 950 mL deionized water.
- Adjust the pH of the medium to 7.0 using 1M NaOH or KOH and bring volume up to 1 liter.
- Autoclave.
- Store at room temperature or +4°C.
LB Agar
Procedure
- follow steps to make lysogeny broth as above
- add 15g of agar powder per litre of LB
- autoclave in 200 or 250 mL aliquots in 500mL duran bottles
- add antibiotic desired to melted agar (~55°C)
- pour into petri dishes and leave to harden in asceptic fume hood
- invert, label, wrap with parafilm and store at 4°C