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| | <!--h2 class="text-center wowload fadeInUp" style="color:#ff0000"><big>Integrated Human Practices</big></h2--> | | <!--h2 class="text-center wowload fadeInUp" style="color:#ff0000"><big>Integrated Human Practices</big></h2--> |
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| − | <h4><i class="fa fa-ils"style="color:#b22222"></i> Introduction </h4>
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| − | <!--p align="justify">The Bio-console is a project developed to make science accessible for all and design for all.
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| − | In this way the safety of the project is one of the most important part.
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| − | Make sure that the bacteria are not able to be release in nature.
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| − | To be sure that any bacteria are able to leave the game different measure have been taken; a kill-swirch, a killing-liposome and a hermetic microfluidic chip.
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| − | </p-->
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| − | <!--Kill Switch -->
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| − | <h3 align="center">Poularization of Biology</h3>
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| − | <h4><i class="fa fa-question"style="color:#b22222"></i>Why do we chose a kill Switch ?</h4>
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| − | <p align="justify"> A kill switch is a <font style="color:#66cdaa"><b>genetically-encoded suicide trigger</b></font>.
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| − | This trigger can be a change in the environment which will make the organism’s life dependent on it.
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| − | In our project, the change will be the presence or not of light.
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| − | That is very interesting as we could create a game over in our Bio Console, once we trigger the bacteria’s death through light.
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| − | Moreover, our team is really concerned about<font style="color:#66cdaa"> BioSafety</font>.
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| − | It is important for us to avoid any possibility of escaping from our microfluidic chip.
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| − | The Kill switch would permit to contain our genetically modified bacteria into the chip through lethality.</p>
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| − | <h4><i class="fa fa-times"style="color:#b22222"></i>How we design it </h4>
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| − | <p align="justify">So we aim to construct an efficient kill-switch triggering by light based on the <font style="color:#66cdaa"><b>pDawn & pDusk - Toxin systems</b></font>.
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| − | In this way we will construct two different parts.
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| − | The first one will be composed of pDawn and <font style="color:#66cdaa"><b>holin/endolysin</b>.</font>
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| − | Toxins will be produced after light stimulation and induce lysis.
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| − | The second part will be composed of pDusk and endolysin/holin complex, this will lead to the cell lysis when the bacteria won’t be exposed to light.
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| − | This second part will be more difficult to characterized indeed all steps before characterization of the plasmid as plasmid amplification will have to be under light. </p>
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