Difference between revisions of "Team:Toulouse/Experiments"

Line 93: Line 93:
 
           <tr>
 
           <tr>
 
             <th>YETM 500mL</th>
 
             <th>YETM 500mL</th>
−
             <th>Header 2</th>
+
             <th>TFB1 200mL</th>
−
             <th>Header 3</th>
+
             <th>TFB2 200mL</th>
 
           </tr>
 
           </tr>
 
         </thead>
 
         </thead>
Line 109: Line 109:
 
</td><!-- YETM 500mL END -->
 
</td><!-- YETM 500mL END -->
 
 
−
<td>Value 5</td>
+
<td><!-- TFB1 200mL -->
 +
<ul style="font-size:15px;">
 +
<li>  0.59g KOAc </li>
 +
<li> 2.42g RbCl </li>
 +
<li> 0.29g CaCl2.2H2O </li>
 +
<li> 1.98g MnCl2.4H2O </li>
 +
<li> Adjust to pH 5.8 with 0.2 M acetic acid </li>
 +
<li> Add dH2O to 200mL </li>
 +
<li> Filter sterilize </li>
 +
<li> Store refrigerated at 4°C </li>
 +
</ul>
 +
</td><!-- TFB1 200mL END -->
 
 
−
<td>Value 9</td>
+
<td><!-- TFB2 200mL -->
 +
<ul style="font-size:15px;">
 +
<li> 2.5g Yeast Extract </li>
 +
<li> 10g Tryptone </li>
 +
<li> 5g MgSO4.7H2O </li>
 +
<li> Adjust pH to 7.5 with KOH </li>
 +
<li> <b>For Plates</b>: add 7.5g of Agar </li>
 +
</ul>
 +
</td><!-- TFB2 200mL END -->
 
           </tr>
 
           </tr>
 
         </tbody>
 
         </tbody>

Revision as of 10:08, 1 September 2015

iGEM Toulouse 2015

Experiments & Protocols


Transformation Protocol: RbCl method

Cloning
YETM 500mL TFB1 200mL TFB2 200mL
  • 2.5g Yeast Extract
  • 10g Tryptone
  • 5g MgSO4.7H2O
  • Adjust pH to 7.5 with KOH
  • For Plates: add 7.5g of Agar
  • 0.59g KOAc
  • 2.42g RbCl
  • 0.29g CaCl2.2H2O
  • 1.98g MnCl2.4H2O
  • Adjust to pH 5.8 with 0.2 M acetic acid
  • Add dH2O to 200mL
  • Filter sterilize
  • Store refrigerated at 4°C
  • 2.5g Yeast Extract
  • 10g Tryptone
  • 5g MgSO4.7H2O
  • Adjust pH to 7.5 with KOH
  • For Plates: add 7.5g of Agar

References