Difference between revisions of "Team:Toulouse/Experiments"

Line 100: Line 100:
 
           <tr>
 
           <tr>
 
             <td><!-- YETM 500mL -->
 
             <td><!-- YETM 500mL -->
−
<ul style="font-size:15px;">
+
<ul style="font-size:15px;"align="left">
 
<li> 2.5g Yeast Extract </li>
 
<li> 2.5g Yeast Extract </li>
 
<li> 10g Tryptone </li>
 
<li> 10g Tryptone </li>
Line 123: Line 123:
 
 
 
<td><!-- TFB2 200mL -->
 
<td><!-- TFB2 200mL -->
−
<ul style="font-size:15px;">
+
<ul style="font-size:15px;"align="left">
−
<li> 2.5g Yeast Extract </li>
+
<li> 0.42g MOPS </li>
−
<li> 10g Tryptone </li>
+
<li> 2.21g CaCl2.2H20 </li>
−
<li> 5g MgSO4.7H2O </li>
+
<li> 0.24g RbCl </li>
−
<li> Adjust pH to 7.5 with KOH </li>
+
<li> 30g Glycerol </li>
−
<li> <b>For Plates</b>: add 7.5g of Agar </li>
+
<li> Adjust to pH 6.5 with KOH </li>
 +
<li> Add dH2O to 200mL </li>
 +
<li> Filter sterilize </li>
 +
<li> Store refrigerated at 4°C </li>
 
</ul>
 
</ul>
 
</td><!-- TFB2 200mL END -->
 
</td><!-- TFB2 200mL END -->

Revision as of 10:11, 1 September 2015

iGEM Toulouse 2015

Experiments & Protocols


Transformation Protocol: RbCl method

Cloning
YETM 500mL TFB1 200mL TFB2 200mL
  • 2.5g Yeast Extract
  • 10g Tryptone
  • 5g MgSO4.7H2O
  • Adjust pH to 7.5 with KOH
  • For Plates: add 7.5g of Agar
  • 0.59g KOAc
  • 2.42g RbCl
  • 0.29g CaCl2.2H2O
  • 1.98g MnCl2.4H2O
  • Adjust to pH 5.8 with 0.2 M acetic acid
  • Add dH2O to 200mL
  • Filter sterilize
  • Store refrigerated at 4°C
  • 0.42g MOPS
  • 2.21g CaCl2.2H20
  • 0.24g RbCl
  • 30g Glycerol
  • Adjust to pH 6.5 with KOH
  • Add dH2O to 200mL
  • Filter sterilize
  • Store refrigerated at 4°C

References