Difference between revisions of "Team:Birkbeck/InterLab Study/supplementary info"

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</head>
 
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<h1>under construction</h1>
 
<h1>under construction</h1>
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<div class="itemBody">
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<table class="Default PivotTable" data-lookName="Default" data-rowLabelWidth="1" data-colLabelHeight="2" data-NumRows="7" data-NumCols="5" data-rowLabels="Corner" data-decimal-char="." data-col-labels="N ;Subset for alpha = 0.05 1; 2; 3; 4" data-row-labels="E. coli DH5 alpha;E. coli DH5 alpha negative control;E. coli DH5 alpha + P3;E. coli DH5 alpha Positive control;E. coli DH5 alpha + P2;E. coli DH5 alpha + P1;Sig." data-maxColWidth="72">
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<caption class="title" style="width:495px">Fluorescence<span class="details">Fluorescence, table, Tukey HSD, 1 layers, 2 levels of column headers and 1 levels of row headers, table with 6 columns and 13 rows</span></caption>
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<td class="defaultLayer layers titleLayerSeparator" colspan="6">
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<tr>
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<td colspan="1">Tukey HSD<sup>a</sup></td>
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<td class="cornerLabels" rowspan="2">Organism</td>
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<td class="columnLabels dataAreaLeft hDC vCC role3" rowspan="2">N</td>
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<td class="columnLabels hCC vCC role2" colspan="4">Subset for alpha = 0.05</td>
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<td class="columnLabels vCC role3">1</td>
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<td class="columnLabels vCC role3">2</td>
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<td class="columnLabels vCC role3">3</td>
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<td class="columnLabels vCC role3">4</td>
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<tr>
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<td class="rowLabels dataAreaTop role3">E. coli DH5 alpha</td>
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<td class=" e dataAreaTop dataAreaLeft vCC">9</td>
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<td class=" e dataAreaTop vCC">4713.00</td>
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<td class=" e dataAreaTop vCC">&nbsp;</td>
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<td class=" e dataAreaTop vCC">&nbsp;</td>
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<td class=" e dataAreaTop vCC">&nbsp;</td>
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<tr>
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<td class="rowLabels hCR role3">E. coli DH5 alpha negative control</td>
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<td class=" o dataAreaLeft hCR vCC">9</td>
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<td class=" o hCR vCC">6418.56</td>
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<td class=" o hCR vCC">&nbsp;</td>
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<td class=" o hCR vCC">&nbsp;</td>
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<td class=" o hCR vCC">&nbsp;</td>
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</tr>
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<tr>
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<td class="rowLabels hCR role3">E. coli DH5 alpha + P3</td>
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<td class=" e dataAreaLeft hCR vCC">9</td>
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<td class=" e hCR vCC">9511.89</td>
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<td class=" e hCR vCC">&nbsp;</td>
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<td class=" e hCR vCC">&nbsp;</td>
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<td class=" e hCR vCC">&nbsp;</td>
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<tr>
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<td class="rowLabels hCR role3">E. coli DH5 alpha Positive control</td>
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<td class=" o dataAreaLeft hCR vCC">9</td>
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<td class=" o hCR vCC">&nbsp;</td>
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<td class=" o hCR vCC">26818.56</td>
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<td class=" o hCR vCC">&nbsp;</td>
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<td class=" o hCR vCC">&nbsp;</td>
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<tr>
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<td class="rowLabels hCR role3">E. coli DH5 alpha + P2</td>
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<td class=" e dataAreaLeft hCR vCC">9</td>
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<td class=" e hCR vCC">&nbsp;</td>
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<td class=" e hCR vCC">&nbsp;</td>
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<td class=" e hCR vCC">40126.89</td>
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<td class=" e hCR vCC">&nbsp;</td>
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</tr>
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<tr>
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<td class="rowLabels hCR role3">E. coli DH5 alpha + P1</td>
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<td class=" o dataAreaLeft hCR vCC">9</td>
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<td class=" o hCR vCC">&nbsp;</td>
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<td class=" o hCR vCC">&nbsp;</td>
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<td class=" o hCR vCC">&nbsp;</td>
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<td class=" o hCR vCC">74270.33</td>
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</tr>
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<tr>
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<td class="rowLabels hCR role3">Sig.</td>
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<td class=" e dataAreaLeft hCR vCC">&nbsp;</td>
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<td class=" e hCR vCC">.243</td>
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<td class=" e hCR vCC">1.000</td>
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<td class=" e hCR vCC">1.000</td>
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<td class=" e hCR vCC">1.000</td>
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<tr>
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<td class="caption" colspan="6">Means for groups in homogeneous subsets are displayed.</td>
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<td class="footnotes" colspan="6">a. Uses Harmonic Mean Sample Size = 9.000.</td>
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Revision as of 14:06, 2 September 2015

iGEM Westminster Logo




Birkbeck iGEM

The Owligos are the first-ever team entered into the international Genetically Engineered Machine (iGEM) Competition by Birkbeck, University of London. We’re a varied group of students who reflect the diversity and unique character of our institution: many of us have chosen science as a second career, having already spent some time in full-time work. For most of us, this has meant making our way through a degree while continuing to work full-time. Hopefully this kind of dedication will help us successfully navigate our way through our iGEM project.

Project Aim

Our project aims to create a new diagnostic solution that will be low-tech and cost-effective enough to allow its usage in deprived and remote communities. We’re attempting to engineer a bacteriophage lambda chassis to change its host affinity, while simultaneously adding a marker that will facilitate easy detection of a target bacterial pathogen in patient samples.

To demonstrate this approach as a proof of concept for the competition, we plan to change this affinity between different strains of E.coli; however, ultimately we hope to demonstrate that this principle could also be applied to alter the phage’s host range to other bacterial species. We could then provide a modular system capable of diagnosing a range of diseases. Of course, we haven’t chosen a simple goal. But as Birkbeck pioneers, we are determined to prove ourselves by making our project a success. We can’t wait to present the results of our work at the Giant Jamboree in September!




under construction

FluorescenceFluorescence, table, Tukey HSD, 1 layers, 2 levels of column headers and 1 levels of row headers, table with 6 columns and 13 rows
Tukey HSDa
Organism N Subset for alpha = 0.05
1 2 3 4
E. coli DH5 alpha 9 4713.00      
E. coli DH5 alpha negative control 9 6418.56      
E. coli DH5 alpha + P3 9 9511.89      
E. coli DH5 alpha Positive control 9   26818.56    
E. coli DH5 alpha + P2 9     40126.89  
E. coli DH5 alpha + P1 9       74270.33
Sig.   .243 1.000 1.000 1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 9.000.