Difference between revisions of "Team:Birkbeck/InterLab Study/supplementary info"

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<h1>under construction</h1>
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<table class="Default PivotTable" data-lookName="Default" data-rowLabelWidth="2" data-colLabelHeight="2" data-NumRows="30" data-NumCols="5" data-rowLabels="Corner" data-decimal-char="." data-col-labels="Mean Difference (I-J) ;Std. Error ;Sig. ;95% Confidence Interval Lower Bound; Upper Bound" data-row-labels="E. coli DH5 alpha E. coli DH5 alpha + P1; E. coli DH5 alpha + P2; E. coli DH5 alpha + P3; E. coli DH5 alpha Positive control; E. coli DH5 alpha negative control;E. coli DH5 alpha + P1 E. coli DH5 alpha; E. coli DH5 alpha + P2; E. coli DH5 alpha + P3; E. coli DH5 alpha Positive control; E. coli DH5 alpha negative control;E. coli DH5 alpha + P2 E. coli DH5 alpha; E. coli DH5 alpha + P1; E. coli DH5 alpha + P3; E. coli DH5 alpha Positive control; E. coli DH5 alpha negative control;E. coli DH5 alpha + P3 E. coli DH5 alpha; E. coli DH5 alpha + P1; E. coli DH5 alpha + P2; E. coli DH5 alpha Positive control; E. coli DH5 alpha negative control;E. coli DH5 alpha Positive control E. coli DH5 alpha; E. coli DH5 alpha + P1; E. coli DH5 alpha + P2; E. coli DH5 alpha + P3; E. coli DH5 alpha negative control;E. coli DH5 alpha negative control E. coli DH5 alpha; E. coli DH5 alpha + P1; E. coli DH5 alpha + P2; E. coli DH5 alpha + P3; E. coli DH5 alpha Positive control" data-maxColWidth="72">
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<caption class="title" style="width:670px">Multiple Comparisons<span class="details">Multiple Comparisons, table, Dependent Variable, Fluorescence, Tukey HSD, 1 layers, 2 levels of column headers and 2 levels of row headers, table with 7 columns and 36 rows</span></caption>
 +
<tr class="layerInfo">
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<td class="defaultLayer layers titleLayerSeparator" colspan="7">
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<table class="defaultLayer">
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<tr>
 +
<td colspan="1">Fluorescence</td>
 +
</tr>
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<tr>
 +
<td colspan="1">Tukey HSD</td>
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</table></td>
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<tbody class="PivotBody defaultLayer innerFrameBd">
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<td class="cornerLabels" rowspan="2">(I) Organism</td>
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<td class="cornerLabels" rowspan="2">(J) Organism</td>
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<td class="columnLabels dataAreaLeft hCC vCC role3" rowspan="2">Mean Difference (I-J)</td>
 +
<td class="columnLabels hCC vCC role3" rowspan="2">Std. Error</td>
 +
<td class="columnLabels hDC vCC role3" rowspan="2">Sig.</td>
 +
<td class="columnLabels hCC vCC role2" colspan="2">95% Confidence Interval</td>
 +
</tr>
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<tr>
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<td class="columnLabels vCC role3">Lower Bound</td>
 +
<td class="columnLabels vCC role3">Upper Bound</td>
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</tr>
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<tr>
 +
<td class="rowLabels dataAreaTop role3" rowspan="5">E. coli DH5 alpha</td>
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<td class="rowLabels dataAreaTop vCR role3">E. coli DH5 alpha + P1</td>
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<td class=" o dataAreaTop dataAreaLeft vCC" title="*. The mean difference is significant at the 0.05 level.">-69557.333<sup>*</sup></td>
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<td class=" o dataAreaTop vCC">2150.035</td>
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<td class=" o dataAreaTop vCC">.000</td>
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<td class=" o dataAreaTop vCC">-75938.41</td>
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<td class=" o dataAreaTop vCC">-63176.25</td>
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<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P2</td>
 +
<td class=" e dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">-35413.889<sup>*</sup></td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.000</td>
 +
<td class=" e hCR vCC">-41794.97</td>
 +
<td class=" e hCR vCC">-29032.81</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P3</td>
 +
<td class=" o dataAreaLeft hCR vCC">-4798.889</td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.243</td>
 +
<td class=" o hCR vCC">-11179.97</td>
 +
<td class=" o hCR vCC">1582.19</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha Positive control</td>
 +
<td class=" e dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">-22105.556<sup>*</sup></td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.000</td>
 +
<td class=" e hCR vCC">-28486.63</td>
 +
<td class=" e hCR vCC">-15724.48</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha negative control</td>
 +
<td class=" o dataAreaLeft hCR vCC">-1705.556</td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.967</td>
 +
<td class=" o hCR vCC">-8086.63</td>
 +
<td class=" o hCR vCC">4675.52</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR role3" rowspan="5">E. coli DH5 alpha + P1</td>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha</td>
 +
<td class=" e dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">69557.333<sup>*</sup></td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.000</td>
 +
<td class=" e hCR vCC">63176.25</td>
 +
<td class=" e hCR vCC">75938.41</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P2</td>
 +
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">34143.444<sup>*</sup></td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.000</td>
 +
<td class=" o hCR vCC">27762.37</td>
 +
<td class=" o hCR vCC">40524.52</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P3</td>
 +
<td class=" e dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">64758.444<sup>*</sup></td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.000</td>
 +
<td class=" e hCR vCC">58377.37</td>
 +
<td class=" e hCR vCC">71139.52</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha Positive control</td>
 +
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">47451.778<sup>*</sup></td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.000</td>
 +
<td class=" o hCR vCC">41070.70</td>
 +
<td class=" o hCR vCC">53832.86</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha negative control</td>
 +
<td class=" e dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">67851.778<sup>*</sup></td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.000</td>
 +
<td class=" e hCR vCC">61470.70</td>
 +
<td class=" e hCR vCC">74232.86</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR role3" rowspan="5">E. coli DH5 alpha + P2</td>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha</td>
 +
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">35413.889<sup>*</sup></td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.000</td>
 +
<td class=" o hCR vCC">29032.81</td>
 +
<td class=" o hCR vCC">41794.97</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P1</td>
 +
<td class=" e dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">-34143.444<sup>*</sup></td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.000</td>
 +
<td class=" e hCR vCC">-40524.52</td>
 +
<td class=" e hCR vCC">-27762.37</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P3</td>
 +
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">30615.000<sup>*</sup></td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.000</td>
 +
<td class=" o hCR vCC">24233.92</td>
 +
<td class=" o hCR vCC">36996.08</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha Positive control</td>
 +
<td class=" e dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">13308.333<sup>*</sup></td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.000</td>
 +
<td class=" e hCR vCC">6927.25</td>
 +
<td class=" e hCR vCC">19689.41</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha negative control</td>
 +
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">33708.333<sup>*</sup></td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.000</td>
 +
<td class=" o hCR vCC">27327.25</td>
 +
<td class=" o hCR vCC">40089.41</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR role3" rowspan="5">E. coli DH5 alpha + P3</td>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha</td>
 +
<td class=" e dataAreaLeft hCR vCC">4798.889</td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.243</td>
 +
<td class=" e hCR vCC">-1582.19</td>
 +
<td class=" e hCR vCC">11179.97</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P1</td>
 +
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">-64758.444<sup>*</sup></td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.000</td>
 +
<td class=" o hCR vCC">-71139.52</td>
 +
<td class=" o hCR vCC">-58377.37</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P2</td>
 +
<td class=" e dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">-30615.000<sup>*</sup></td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.000</td>
 +
<td class=" e hCR vCC">-36996.08</td>
 +
<td class=" e hCR vCC">-24233.92</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha Positive control</td>
 +
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">-17306.667<sup>*</sup></td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.000</td>
 +
<td class=" o hCR vCC">-23687.75</td>
 +
<td class=" o hCR vCC">-10925.59</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha negative control</td>
 +
<td class=" e dataAreaLeft hCR vCC">3093.333</td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.704</td>
 +
<td class=" e hCR vCC">-3287.75</td>
 +
<td class=" e hCR vCC">9474.41</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR role3" rowspan="5">E. coli DH5 alpha Positive control</td>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha</td>
 +
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">22105.556<sup>*</sup></td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.000</td>
 +
<td class=" o hCR vCC">15724.48</td>
 +
<td class=" o hCR vCC">28486.63</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P1</td>
 +
<td class=" e dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">-47451.778<sup>*</sup></td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.000</td>
 +
<td class=" e hCR vCC">-53832.86</td>
 +
<td class=" e hCR vCC">-41070.70</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P2</td>
 +
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">-13308.333<sup>*</sup></td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.000</td>
 +
<td class=" o hCR vCC">-19689.41</td>
 +
<td class=" o hCR vCC">-6927.25</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P3</td>
 +
<td class=" e dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">17306.667<sup>*</sup></td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.000</td>
 +
<td class=" e hCR vCC">10925.59</td>
 +
<td class=" e hCR vCC">23687.75</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha negative control</td>
 +
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">20400.000<sup>*</sup></td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.000</td>
 +
<td class=" o hCR vCC">14018.92</td>
 +
<td class=" o hCR vCC">26781.08</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR role3" rowspan="5">E. coli DH5 alpha negative control</td>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha</td>
 +
<td class=" e dataAreaLeft hCR vCC">1705.556</td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.967</td>
 +
<td class=" e hCR vCC">-4675.52</td>
 +
<td class=" e hCR vCC">8086.63</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P1</td>
 +
<td class=" o dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">-67851.778<sup>*</sup></td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.000</td>
 +
<td class=" o hCR vCC">-74232.86</td>
 +
<td class=" o hCR vCC">-61470.70</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P2</td>
 +
<td class=" e dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">-33708.333<sup>*</sup></td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.000</td>
 +
<td class=" e hCR vCC">-40089.41</td>
 +
<td class=" e hCR vCC">-27327.25</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha + P3</td>
 +
<td class=" o dataAreaLeft hCR vCC">-3093.333</td>
 +
<td class=" o hCR vCC">2150.035</td>
 +
<td class=" o hCR vCC">.704</td>
 +
<td class=" o hCR vCC">-9474.41</td>
 +
<td class=" o hCR vCC">3287.75</td>
 +
</tr>
 +
<tr>
 +
<td class="rowLabels hCR vCR role3">E. coli DH5 alpha Positive control</td>
 +
<td class=" e dataAreaLeft hCR vCC" title="*. The mean difference is significant at the 0.05 level.">-20400.000<sup>*</sup></td>
 +
<td class=" e hCR vCC">2150.035</td>
 +
<td class=" e hCR vCC">.000</td>
 +
<td class=" e hCR vCC">-26781.08</td>
 +
<td class=" e hCR vCC">-14018.92</td>
 +
</tr>
 +
</tbody><tfoot style="display:table-footer-group">
 +
<tr>
 +
<td class="footnotes" colspan="7">*. The mean difference is significant at the 0.05 level.</td>
 +
</tr>
 +
<tr>
 +
<td  style="width:120px"></td>
 +
<td  style="width:120px"></td>
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<td  style="width:96px"></td>
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<td  style="width:79px"></td>
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<td  style="width:67px"></td>
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<td  style="width:93px"></td>
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<td  style="width:95px"></td>
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</tfoot>
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</table>
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</div><CENTER><H1><b><head><style type="text/css">p{color:0;font-family:SansSerif;font-size:18pt;font-style:normal;font-weight:bold;text-decoration:none}</style></head><BR>Homogeneous&nbsp;Subsets</b></H1></CENTER><BR>

Latest revision as of 14:48, 2 September 2015

iGEM Westminster Logo




Birkbeck iGEM

The Owligos are the first-ever team entered into the international Genetically Engineered Machine (iGEM) Competition by Birkbeck, University of London. We’re a varied group of students who reflect the diversity and unique character of our institution: many of us have chosen science as a second career, having already spent some time in full-time work. For most of us, this has meant making our way through a degree while continuing to work full-time. Hopefully this kind of dedication will help us successfully navigate our way through our iGEM project.

Project Aim

Our project aims to create a new diagnostic solution that will be low-tech and cost-effective enough to allow its usage in deprived and remote communities. We’re attempting to engineer a bacteriophage lambda chassis to change its host affinity, while simultaneously adding a marker that will facilitate easy detection of a target bacterial pathogen in patient samples.

To demonstrate this approach as a proof of concept for the competition, we plan to change this affinity between different strains of E.coli; however, ultimately we hope to demonstrate that this principle could also be applied to alter the phage’s host range to other bacterial species. We could then provide a modular system capable of diagnosing a range of diseases. Of course, we haven’t chosen a simple goal. But as Birkbeck pioneers, we are determined to prove ourselves by making our project a success. We can’t wait to present the results of our work at the Giant Jamboree in September!




Multiple ComparisonsMultiple Comparisons, table, Dependent Variable, Fluorescence, Tukey HSD, 1 layers, 2 levels of column headers and 2 levels of row headers, table with 7 columns and 36 rows
Fluorescence
Tukey HSD
(I) Organism (J) Organism Mean Difference (I-J) Std. Error Sig. 95% Confidence Interval
Lower Bound Upper Bound
E. coli DH5 alpha E. coli DH5 alpha + P1 -69557.333* 2150.035 .000 -75938.41 -63176.25
E. coli DH5 alpha + P2 -35413.889* 2150.035 .000 -41794.97 -29032.81
E. coli DH5 alpha + P3 -4798.889 2150.035 .243 -11179.97 1582.19
E. coli DH5 alpha Positive control -22105.556* 2150.035 .000 -28486.63 -15724.48
E. coli DH5 alpha negative control -1705.556 2150.035 .967 -8086.63 4675.52
E. coli DH5 alpha + P1 E. coli DH5 alpha 69557.333* 2150.035 .000 63176.25 75938.41
E. coli DH5 alpha + P2 34143.444* 2150.035 .000 27762.37 40524.52
E. coli DH5 alpha + P3 64758.444* 2150.035 .000 58377.37 71139.52
E. coli DH5 alpha Positive control 47451.778* 2150.035 .000 41070.70 53832.86
E. coli DH5 alpha negative control 67851.778* 2150.035 .000 61470.70 74232.86
E. coli DH5 alpha + P2 E. coli DH5 alpha 35413.889* 2150.035 .000 29032.81 41794.97
E. coli DH5 alpha + P1 -34143.444* 2150.035 .000 -40524.52 -27762.37
E. coli DH5 alpha + P3 30615.000* 2150.035 .000 24233.92 36996.08
E. coli DH5 alpha Positive control 13308.333* 2150.035 .000 6927.25 19689.41
E. coli DH5 alpha negative control 33708.333* 2150.035 .000 27327.25 40089.41
E. coli DH5 alpha + P3 E. coli DH5 alpha 4798.889 2150.035 .243 -1582.19 11179.97
E. coli DH5 alpha + P1 -64758.444* 2150.035 .000 -71139.52 -58377.37
E. coli DH5 alpha + P2 -30615.000* 2150.035 .000 -36996.08 -24233.92
E. coli DH5 alpha Positive control -17306.667* 2150.035 .000 -23687.75 -10925.59
E. coli DH5 alpha negative control 3093.333 2150.035 .704 -3287.75 9474.41
E. coli DH5 alpha Positive control E. coli DH5 alpha 22105.556* 2150.035 .000 15724.48 28486.63
E. coli DH5 alpha + P1 -47451.778* 2150.035 .000 -53832.86 -41070.70
E. coli DH5 alpha + P2 -13308.333* 2150.035 .000 -19689.41 -6927.25
E. coli DH5 alpha + P3 17306.667* 2150.035 .000 10925.59 23687.75
E. coli DH5 alpha negative control 20400.000* 2150.035 .000 14018.92 26781.08
E. coli DH5 alpha negative control E. coli DH5 alpha 1705.556 2150.035 .967 -4675.52 8086.63
E. coli DH5 alpha + P1 -67851.778* 2150.035 .000 -74232.86 -61470.70
E. coli DH5 alpha + P2 -33708.333* 2150.035 .000 -40089.41 -27327.25
E. coli DH5 alpha + P3 -3093.333 2150.035 .704 -9474.41 3287.75
E. coli DH5 alpha Positive control -20400.000* 2150.035 .000 -26781.08 -14018.92
*. The mean difference is significant at the 0.05 level.


Homogeneous Subsets