Revision as of 10:58, 3 September 2015

Birkbeck iGEM

The Owligos are the first-ever team entered into the international Genetically Engineered Machine (iGEM) Competition by Birkbeck, University of London. We’re a varied group of students who reflect the diversity and unique character of our institution: many of us have chosen science as a second career, having already spent some time in full-time work. For most of us, this has meant making our way through a degree while continuing to work full-time. Hopefully this kind of dedication will help us successfully navigate our way through our iGEM project.

Project Aim

Our project aims to create a new diagnostic solution that will be low-tech and cost-effective enough to allow its usage in deprived and remote communities. We’re attempting to engineer a bacteriophage lambda chassis to change its host affinity, while simultaneously adding a marker that will facilitate easy detection of a target bacterial pathogen in patient samples.

To demonstrate this approach as a proof of concept for the competition, we plan to change this affinity between different strains of E.coli; however, ultimately we hope to demonstrate that this principle could also be applied to alter the phage’s host range to other bacterial species. We could then provide a modular system capable of diagnosing a range of diseases. Of course, we haven’t chosen a simple goal. But as Birkbeck pioneers, we are determined to prove ourselves by making our project a success. We can’t wait to present the results of our work at the Giant Jamboree in September!

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+<td class=" o dataAreaLeft hCR vCC">33695218241.426</td>
+<td class=" o hCR vCC">53</td>
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E. coli DH5 alpha (second triplicate); E. coli DH5 alpha (third triplicate); E. coli DH5 alpha P1 (first triplicate); E. coli DH5 alpha P1 (second triplicate); E. coli DH5 alpha P1 (third triplicate); E. coli DH5 alpha P2 (first triplicate); E. coli DH5 alpha P2 (second triplicate); E. coli DH5 alpha P2 (third triplicate); E. coli DH5 alpha P3 (first triplicate); E. coli DH5 alpha P3 (third triplicate); E. coli DH5 alpha Positive control (first triplicate); E. coli DH5 alpha Positive control (second triplicate); E. coli DH5 alpha Positive control (third triplicate); E. coli DH5 alpha negative control (first triplicate); E. coli DH5 alpha negative control (second triplicate); E. coli DH5 alpha negative control (third triplicate);E. coli DH5 alpha P3 (third triplicate) E. coli DH5 alpha (first triplicate); E. coli DH5 alpha (second triplicate); E. coli DH5 alpha (third triplicate); E. coli DH5 alpha P1 (first triplicate); E. coli DH5 alpha P1 (second triplicate); E. coli DH5 alpha P1 (third triplicate); E. coli DH5 alpha P2 (first triplicate); E. coli DH5 alpha P2 (second triplicate); E. coli DH5 alpha P2 (third triplicate); E. coli DH5 alpha P3 (first triplicate); E. coli DH5 alpha P3 (second triplicate); E. coli DH5 alpha Positive control (first triplicate); E. coli DH5 alpha Positive control (second triplicate); E. coli DH5 alpha Positive control (third triplicate); E. coli DH5 alpha negative control (first triplicate); E. coli DH5 alpha negative control (second triplicate); E. coli DH5 alpha negative control (third triplicate);E. coli DH5 alpha Positive control (first triplicate) E. coli DH5 alpha (first triplicate); E. coli DH5 alpha (second triplicate); E. coli DH5 alpha (third triplicate); E. coli DH5 alpha P1 (first triplicate); E. coli DH5 alpha P1 (second triplicate); E. coli DH5 alpha P1 (third triplicate); E. coli DH5 alpha P2 (first triplicate); E. coli DH5 alpha P2 (second triplicate); E. coli DH5 alpha P2 (third triplicate); E. coli DH5 alpha P3 (first triplicate); E. coli DH5 alpha P3 (second triplicate); E. coli DH5 alpha P3 (third triplicate); E. coli DH5 alpha Positive control (second triplicate); E. coli DH5 alpha Positive control (third triplicate); E. coli DH5 alpha negative control (first triplicate); E. coli DH5 alpha negative control (second triplicate); E. coli DH5 alpha negative control (third triplicate);E. coli DH5 alpha Positive control (second triplicate) E. coli DH5 alpha (first triplicate); E. coli DH5 alpha (second triplicate); E. coli DH5 alpha (third triplicate); E. coli DH5 alpha P1 (first triplicate); E. coli DH5 alpha P1 (second triplicate); E. coli DH5 alpha P1 (third triplicate); E. coli DH5 alpha P2 (first triplicate); E. coli DH5 alpha P2 (second triplicate); E. coli DH5 alpha P2 (third triplicate); E. coli DH5 alpha P3 (first triplicate); E. coli DH5 alpha P3 (second triplicate); E. coli DH5 alpha P3 (third triplicate); E. coli DH5 alpha Positive control (first triplicate); E. coli DH5 alpha Positive control (third triplicate); E. coli DH5 alpha negative control (first triplicate); E. coli DH5 alpha negative control (second triplicate); E. coli DH5 alpha negative control (third triplicate);E. coli DH5 alpha Positive control (third triplicate) E. coli DH5 alpha (first triplicate); E. coli DH5 alpha (second triplicate); E. coli DH5 alpha (third triplicate); E. coli DH5 alpha P1 (first triplicate); E. coli DH5 alpha P1 (second triplicate); E. coli DH5 alpha P1 (third triplicate); E. coli DH5 alpha P2 (first triplicate); E. coli DH5 alpha P2 (second triplicate); E. coli DH5 alpha P2 (third triplicate); E. coli DH5 alpha P3 (first triplicate); E. coli DH5 alpha P3 (second triplicate); E. coli DH5 alpha P3 (third triplicate); E. coli DH5 alpha Positive control (first triplicate); E. coli DH5 alpha Positive control (second triplicate); E. coli DH5 alpha negative control (first triplicate); E. coli DH5 alpha negative control (second triplicate); E. coli DH5 alpha negative control (third triplicate);E. coli DH5 alpha negative control (first triplicate) E. coli DH5 alpha (first triplicate); E. coli DH5 alpha (second triplicate); E. coli DH5 alpha (third triplicate); E. coli DH5 alpha P1 (first triplicate); E. coli DH5 alpha P1 (second triplicate); E. coli DH5 alpha P1 (third triplicate); E. coli DH5 alpha P2 (first triplicate); E. coli DH5 alpha P2 (second triplicate); E. coli DH5 alpha P2 (third triplicate); E. coli DH5 alpha P3 (first triplicate); E. coli DH5 alpha P3 (second triplicate); E. coli DH5 alpha P3 (third triplicate); E. coli DH5 alpha Positive control (first triplicate); E. coli DH5 alpha Positive control (second triplicate); E. coli DH5 alpha Positive control (third triplicate); E. coli DH5 alpha negative control (second triplicate); E. coli DH5 alpha negative control (third triplicate);E. coli DH5 alpha negative control (second triplicate) E. coli DH5 alpha (first triplicate); E. coli DH5 alpha (second triplicate); E. coli DH5 alpha (third triplicate); E. coli DH5 alpha P1 (first triplicate); E. coli DH5 alpha P1 (second triplicate); E. coli DH5 alpha P1 (third triplicate); E. coli DH5 alpha P2 (first triplicate); E. coli DH5 alpha P2 (second triplicate); E. coli DH5 alpha P2 (third triplicate); E. coli DH5 alpha P3 (first triplicate); E. coli DH5 alpha P3 (second triplicate); E. coli DH5 alpha P3 (third triplicate); E. coli DH5 alpha Positive control (first triplicate); E. coli DH5 alpha Positive control (second triplicate); E. coli DH5 alpha Positive control (third triplicate); E. coli DH5 alpha negative control (first triplicate); E. coli DH5 alpha negative control (third triplicate);E. coli DH5 alpha negative control (third triplicate) E. coli DH5 alpha (first triplicate); E. coli DH5 alpha (second triplicate); E. coli DH5 alpha (third triplicate); E. coli DH5 alpha P1 (first triplicate); E. coli DH5 alpha P1 (second triplicate); E. coli DH5 alpha P1 (third triplicate); E. coli DH5 alpha P2 (first triplicate); E. coli DH5 alpha P2 (second triplicate); E. coli DH5 alpha P2 (third triplicate); E. coli DH5 alpha P3 (first triplicate); E. coli DH5 alpha P3 (second triplicate); E. coli DH5 alpha P3 (third triplicate); E. coli DH5 alpha Positive control (first triplicate); E. coli DH5 alpha Positive control (second triplicate); E. coli DH5 alpha Positive control (third triplicate); E. coli DH5 alpha negative control (first triplicate); E. coli DH5 alpha negative control (second triplicate)" data-maxColWidth="72">
-<caption class="title" style="width:670px">Multiple Comparisons<span class="details">Multiple Comparisons of Fluorescence (ungrouped)</span></caption>
+<caption class="title" style="width:670px"><span class="details">Multiple Comparisons of Fluorescence of <i>E. coli</i> DH5α Strains.</span></caption>
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@@ Line 2,661: / Line 3,084: @@
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+</div>
+<!--<div class="itemBody">
+<table class="Default PivotTable" data-lookName="Default" data-rowLabelWidth="1" data-colLabelHeight="2" data-NumRows="19" data-NumCols="6" data-rowLabels="Corner" data-decimal-char="." data-col-labels="N ;Subset for alpha = 0.05 1; 2; 3; 4; 5" data-row-labels="E. coli DH5 alpha (third triplicate);E. coli DH5 alpha (first triplicate);E. coli DH5 alpha (second triplicate);E. coli DH5 alpha negative control (second triplicate);E. coli DH5 alpha negative control (third triplicate);E. coli DH5 alpha negative control (first triplicate);E. coli DH5 alpha P3 (third triplicate);E. coli DH5 alpha P3 (first triplicate);E. coli DH5 alpha P3 (second triplicate);E. coli DH5 alpha Positive control (first triplicate);E. coli DH5 alpha Positive control (second triplicate);E. coli DH5 alpha Positive control (third triplicate);E. coli DH5 alpha P2 (first triplicate);E. coli DH5 alpha P2 (third triplicate);E. coli DH5 alpha P2 (second triplicate);E. coli DH5 alpha P1 (third triplicate);E. coli DH5 alpha P1 (second triplicate);E. coli DH5 alpha P1 (first triplicate);Sig." data-maxColWidth="72">
+<caption class="title" style="width:582px">Fluorescence<span class="details">Fluorescence, table, Tukey HSD, 1 layers, 2 levels of column headers and 1 levels of row headers, table with 7 columns and 25 rows</span></caption>
+<tr class="layerInfo">
+<td class="defaultLayer layers titleLayerSeparator" colspan="7">
+<table class="defaultLayer">
+<tr>
+<td colspan="1">Tukey HSD<sup>a</sup></td>
+</tr>
+</table></td>
+</tr>
+<tbody class="PivotBody defaultLayer innerFrameBd">
+<tr>
+<td class="cornerLabels" rowspan="2">GroupingTtest</td>
+<td class="columnLabels dataAreaLeft hDC vCC role3" rowspan="2">N</td>
+<td class="columnLabels hCC vCC role2" colspan="5">Subset for alpha = 0.05</td>
+</tr>
+<tr>
+<td class="columnLabels vCC role3">1</td>
+<td class="columnLabels vCC role3">2</td>
+<td class="columnLabels vCC role3">3</td>
+<td class="columnLabels vCC role3">4</td>
+<td class="columnLabels vCC role3">5</td>
+</tr>
+<tr>
+<td class="rowLabels dataAreaTop role3">E. coli DH5 alpha (third triplicate)</td>
+<td class=" e dataAreaTop dataAreaLeft vCC">3</td>
+<td class=" e dataAreaTop vCC">4593.67</td>
+<td class=" e dataAreaTop vCC">&nbsp;</td>
+<td class=" e dataAreaTop vCC">&nbsp;</td>
+<td class=" e dataAreaTop vCC">&nbsp;</td>
+<td class=" e dataAreaTop vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha (first triplicate)</td>
+<td class=" o dataAreaLeft hCR vCC">3</td>
+<td class=" o hCR vCC">4606.67</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha (second triplicate)</td>
+<td class=" e dataAreaLeft hCR vCC">3</td>
+<td class=" e hCR vCC">4938.67</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha negative control (second triplicate)</td>
+<td class=" o dataAreaLeft hCR vCC">3</td>
+<td class=" o hCR vCC">6087.33</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha negative control (third triplicate)</td>
+<td class=" e dataAreaLeft hCR vCC">3</td>
+<td class=" e hCR vCC">6481.67</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha negative control (first triplicate)</td>
+<td class=" o dataAreaLeft hCR vCC">3</td>
+<td class=" o hCR vCC">6686.67</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha P3 (third triplicate)</td>
+<td class=" e dataAreaLeft hCR vCC">3</td>
+<td class=" e hCR vCC">9051.00</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha P3 (first triplicate)</td>
+<td class=" o dataAreaLeft hCR vCC">3</td>
+<td class=" o hCR vCC">9056.33</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha P3 (second triplicate)</td>
+<td class=" e dataAreaLeft hCR vCC">3</td>
+<td class=" e hCR vCC">10428.33</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha Positive control (first triplicate)</td>
+<td class=" o dataAreaLeft hCR vCC">3</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">26246.00</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha Positive control (second triplicate)</td>
+<td class=" e dataAreaLeft hCR vCC">3</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">27068.00</td>
+<td class=" e hCR vCC">27068.00</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha Positive control (third triplicate)</td>
+<td class=" o dataAreaLeft hCR vCC">3</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">27141.67</td>
+<td class=" o hCR vCC">27141.67</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha P2 (first triplicate)</td>
+<td class=" e dataAreaLeft hCR vCC">3</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">35056.67</td>
+<td class=" e hCR vCC">35056.67</td>
+<td class=" e hCR vCC">35056.67</td>
+<td class=" e hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha P2 (third triplicate)</td>
+<td class=" o dataAreaLeft hCR vCC">3</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">39823.67</td>
+<td class=" o hCR vCC">39823.67</td>
+<td class=" o hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha P2 (second triplicate)</td>
+<td class=" e dataAreaLeft hCR vCC">3</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">45500.33</td>
+<td class=" e hCR vCC">&nbsp;</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha P1 (third triplicate)</td>
+<td class=" o dataAreaLeft hCR vCC">3</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">70725.67</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha P1 (second triplicate)</td>
+<td class=" e dataAreaLeft hCR vCC">3</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">72252.67</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">E. coli DH5 alpha P1 (first triplicate)</td>
+<td class=" o dataAreaLeft hCR vCC">3</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">&nbsp;</td>
+<td class=" o hCR vCC">79832.67</td>
+</tr>
+<tr>
+<td class="rowLabels hCR role3">Sig.</td>
+<td class=" e dataAreaLeft hCR vCC">&nbsp;</td>
+<td class=" e hCR vCC">.967</td>
+<td class=" e hCR vCC">.563</td>
+<td class=" e hCR vCC">.075</td>
+<td class=" e hCR vCC">.285</td>
+<td class=" e hCR vCC">.508</td>
+</tr>
+</tbody><tfoot style="display:table-footer-group">
+<tr>
+<td class="caption" colspan="7">Means for groups in homogeneous subsets are displayed.</td>
+</tr>
+<tr>
+<td class="footnotes" colspan="7">a. Uses Harmonic Mean Sample Size = 3.000.</td>
+</tr>
+<tr>
+<td  style="width:120px"></td>
+<td  style="width:67px"></td>
+<td  style="width:79px"></td>
+<td  style="width:79px"></td>
+<td  style="width:79px"></td>
+<td  style="width:79px"></td>
+<td  style="width:79px"></td>
+</tr>
+</tfoot>
+</table>-->
+</div></body></html>

Difference between revisions of "Team:Birkbeck/InterLab Study/fluorescence ANOVA ungrouped"

Revision as of 10:58, 3 September 2015

Birkbeck iGEM

Project Aim