Difference between revisions of "Team:Birkbeck/InterLab Study/fluorescence ANOVA"

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<title>ANOVA of Fluoresce between strains of E. coli grouped1.htm</title>
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<caption class="title" style="width:402px">Notes<span class="details">Notes, table, 0 levels of column headers and 2 levels of row headers, table with 3 columns and 14 rows</span></caption>
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<td class="rowLabels dataAreaTop role3" colspan="2">Output Created</td>
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<td class=" o dataAreaTop dataAreaLeft">03-SEP-2015 12:01:37</td>
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<td class=" e dataAreaLeft hCR" style="text-align:left;">&nbsp;</td>
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<td class="rowLabels hCR role2" rowspan="6">Input</td>
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<td class="rowLabels hCR vCR role3">Data</td>
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<td class=" o dataAreaLeft hCR" style="text-align:left;">/Volumes/HP v150w/Interlab study write-up/Interlab fluorescence data.sav</td>
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<td class="rowLabels hCR vCR role3">Active Dataset</td>
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<td class=" e dataAreaLeft hCR" style="text-align:left;">DataSet1</td>
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<td class="rowLabels hCR vCR role3">N of Rows in Working Data File</td>
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<td class=" e dataAreaLeft hCR">54</td>
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<td class="rowLabels hCR role2" rowspan="2">Missing Value Handling</td>
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<td class=" o dataAreaLeft hCR" style="text-align:left;">User-defined missing values are treated as missing.</td>
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<td class=" e dataAreaLeft hCR" style="text-align:left;">Statistics for each analysis are based on cases with no missing data for any variable in the analysis.</td>
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<td class="rowLabels hCR role3" colspan="2">Syntax</td>
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<td class=" o dataAreaLeft hCR" style="text-align:left;">ONEWAY Fluorescence BY Organism<br>  /MISSING ANALYSIS<br>  /POSTHOC=TUKEY ALPHA(0.05).</td>
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<td class="rowLabels hCR role2" rowspan="2">Resources</td>
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<td class=" e dataAreaLeft hCR">00:00:00.02</td>
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<td class="rowLabels hCR vCR role3">Elapsed Time</td>
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<td class=" o dataAreaLeft hCR">00:00:00.00</td>
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<caption class="title" style="width:510px"><span class="details">ANOVA Table of Grouped of <i>E. coli</i> DH5α Strains Fluorescence.</span></caption>
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<td colspan="1">Fluorescence</td>
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<td class="cornerLabels">&nbsp;</td>
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<td class="columnLabels dataAreaLeft vCC role3">Sum of Squares</td>
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<td class="columnLabels vCC role3">df</td>
 +
<td class="columnLabels vCC role3">Mean Square</td>
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<td class="columnLabels vCC role3">F</td>
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<td class="columnLabels vCC role3">Sig.</td>
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<td class="rowLabels dataAreaTop role3">Between Groups</td>
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<td class=" o dataAreaTop dataAreaLeft vCC">32696725491.204</td>
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<td class=" o dataAreaTop vCC">5</td>
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<td class=" o dataAreaTop vCC">6539345098.241</td>
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<td class=" o dataAreaTop vCC">314.362</td>
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<td class=" o dataAreaTop vCC">.000</td>
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<td class="rowLabels hCR role3">Within Groups</td>
 +
<td class=" e dataAreaLeft hCR vCC">998492750.222</td>
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<td class=" e hCR vCC">48</td>
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<td class=" e hCR vCC">20801932.296</td>
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<td class=" e hCR vCC">&nbsp;</td>
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<td class=" e hCR vCC">&nbsp;</td>
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<td class="rowLabels hCR role3">Total</td>
 +
<td class=" o dataAreaLeft hCR vCC">33695218241.426</td>
 +
<td class=" o hCR vCC">53</td>
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<td class=" o hCR vCC">&nbsp;</td>
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<caption class="title" style="width:670px">Multiple Comparisons<span class="details">Multiple Comparisons of Fluorescence</span></caption>
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<caption class="title" style="width:670px"><span class="details">Multiple Comparisons of Grouped <i>E. coli</i> DH5α Strains Fluorescence table.</span></caption>
 
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<td class="columnLabels vCC role3">2</td>
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<td class=" e dataAreaTop vCC">&nbsp;</td>
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<td class=" e dataAreaTop vCC">&nbsp;</td>
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<td class=" e dataAreaLeft hCR vCC">9</td>
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<td class=" e hCR vCC">9511.89</td>
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<td class=" o dataAreaLeft hCR vCC">9</td>
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<td class=" o hCR vCC">&nbsp;</td>
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<td class=" o hCR vCC">26818.56</td>
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<td class=" o hCR vCC">&nbsp;</td>
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<td class=" e hCR vCC">40126.89</td>
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<td class=" o hCR vCC">&nbsp;</td>
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<td class=" o hCR vCC">74270.33</td>
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<td class="rowLabels hCR role3">Sig.</td>
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<td class=" e hCR vCC">.243</td>
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<td class=" e hCR vCC">1.000</td>
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<td class=" e hCR vCC">1.000</td>
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<td class=" e hCR vCC">1.000</td>
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<td class="caption" colspan="6">Means for groups in homogeneous subsets are displayed.</td>
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Revision as of 11:08, 3 September 2015

iGEM Westminster Logo




Birkbeck iGEM

The Owligos are the first-ever team entered into the international Genetically Engineered Machine (iGEM) Competition by Birkbeck, University of London. We’re a varied group of students who reflect the diversity and unique character of our institution: many of us have chosen science as a second career, having already spent some time in full-time work. For most of us, this has meant making our way through a degree while continuing to work full-time. Hopefully this kind of dedication will help us successfully navigate our way through our iGEM project.

Project Aim

Our project aims to create a new diagnostic solution that will be low-tech and cost-effective enough to allow its usage in deprived and remote communities. We’re attempting to engineer a bacteriophage lambda chassis to change its host affinity, while simultaneously adding a marker that will facilitate easy detection of a target bacterial pathogen in patient samples.

To demonstrate this approach as a proof of concept for the competition, we plan to change this affinity between different strains of E.coli; however, ultimately we hope to demonstrate that this principle could also be applied to alter the phage’s host range to other bacterial species. We could then provide a modular system capable of diagnosing a range of diseases. Of course, we haven’t chosen a simple goal. But as Birkbeck pioneers, we are determined to prove ourselves by making our project a success. We can’t wait to present the results of our work at the Giant Jamboree in September!




ANOVA of Fluoresce between strains of E. coli grouped1.htm

ANOVA Table of Grouped of E. coli DH5α Strains Fluorescence.
Fluorescence
  Sum of Squares df Mean Square F Sig.
Between Groups 32696725491.204 5 6539345098.241 314.362 .000
Within Groups 998492750.222 48 20801932.296    
Total 33695218241.426 53      

Multiple Comparisons of Grouped E. coli DH5α Strains Fluorescence table.
Fluorescence
Tukey HSD
(I) Organism (J) Organism Mean Difference (I-J) Std. Error Sig. 95% Confidence Interval
Lower Bound Upper Bound
E. coli DH5 alpha E. coli DH5 alpha + P1 -69557.333* 2150.035 .000 -75938.41 -63176.25
E. coli DH5 alpha + P2 -35413.889* 2150.035 .000 -41794.97 -29032.81
E. coli DH5 alpha + P3 -4798.889 2150.035 .243 -11179.97 1582.19
E. coli DH5 alpha Positive control -22105.556* 2150.035 .000 -28486.63 -15724.48
E. coli DH5 alpha negative control -1705.556 2150.035 .967 -8086.63 4675.52
E. coli DH5 alpha + P1 E. coli DH5 alpha 69557.333* 2150.035 .000 63176.25 75938.41
E. coli DH5 alpha + P2 34143.444* 2150.035 .000 27762.37 40524.52
E. coli DH5 alpha + P3 64758.444* 2150.035 .000 58377.37 71139.52
E. coli DH5 alpha Positive control 47451.778* 2150.035 .000 41070.70 53832.86
E. coli DH5 alpha negative control 67851.778* 2150.035 .000 61470.70 74232.86
E. coli DH5 alpha + P2 E. coli DH5 alpha 35413.889* 2150.035 .000 29032.81 41794.97
E. coli DH5 alpha + P1 -34143.444* 2150.035 .000 -40524.52 -27762.37
E. coli DH5 alpha + P3 30615.000* 2150.035 .000 24233.92 36996.08
E. coli DH5 alpha Positive control 13308.333* 2150.035 .000 6927.25 19689.41
E. coli DH5 alpha negative control 33708.333* 2150.035 .000 27327.25 40089.41
E. coli DH5 alpha + P3 E. coli DH5 alpha 4798.889 2150.035 .243 -1582.19 11179.97
E. coli DH5 alpha + P1 -64758.444* 2150.035 .000 -71139.52 -58377.37
E. coli DH5 alpha + P2 -30615.000* 2150.035 .000 -36996.08 -24233.92
E. coli DH5 alpha Positive control -17306.667* 2150.035 .000 -23687.75 -10925.59
E. coli DH5 alpha negative control 3093.333 2150.035 .704 -3287.75 9474.41
E. coli DH5 alpha Positive control E. coli DH5 alpha 22105.556* 2150.035 .000 15724.48 28486.63
E. coli DH5 alpha + P1 -47451.778* 2150.035 .000 -53832.86 -41070.70
E. coli DH5 alpha + P2 -13308.333* 2150.035 .000 -19689.41 -6927.25
E. coli DH5 alpha + P3 17306.667* 2150.035 .000 10925.59 23687.75
E. coli DH5 alpha negative control 20400.000* 2150.035 .000 14018.92 26781.08
E. coli DH5 alpha negative control E. coli DH5 alpha 1705.556 2150.035 .967 -4675.52 8086.63
E. coli DH5 alpha + P1 -67851.778* 2150.035 .000 -74232.86 -61470.70
E. coli DH5 alpha + P2 -33708.333* 2150.035 .000 -40089.41 -27327.25
E. coli DH5 alpha + P3 -3093.333 2150.035 .704 -9474.41 3287.75
E. coli DH5 alpha Positive control -20400.000* 2150.035 .000 -26781.08 -14018.92
*. The mean difference is significant at the 0.05 level.