Difference between revisions of "Team:Paris Bettencourt/Notebook/Idli and micro-organisms"

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<h2 class="date one">July 12th to 14th and 16th to 18th</h2><br><br>
 
<h2 class="date one">July 12th to 14th and 16th to 18th</h2><br><br>
  
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I did grow ''Saccharomyce cerevisiae'' with mCherry and geneticin resistance genes in idli. I added 2 ml of a yeast growth solution (YPD) with an OD600 of 0.455 after the soaking phase and the mixing and grinding of rice and dall together.
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I did grow ''Saccharomyce cerevisiae'' with mCherry and geneticin resistance genes in idli. I added 2 ml of a yeast growth solution (YPD) with an OD600 of 0.455 (around 1.2 x 10<sup>7</sup> after the soaking phase and the mixing and grinding of rice and dall together.
  
  

Revision as of 14:16, 3 September 2015

July 2nd to 9th



I experiented different recipes to make idli come from India. I tried to do it with different rice and lentill (called dall in india). Finally, I chose to do this recipe with basmati rice and indian dall.

July 12th to 14th and 16th to 18th



I did grow ''Saccharomyce cerevisiae'' with mCherry and geneticin resistance genes in idli. I added 2 ml of a yeast growth solution (YPD) with an OD600 of 0.455 (around 1.2 x 107 after the soaking phase and the mixing and grinding of rice and dall together.

July 29th



Test of the titration protocol with spectrophotometer for the Vitamin A (protocol titration)
I tested this protocol and tried to calibrate it. I used, like food sample, 1g of rice and I did 8 bottles with 8 different concentrations of pure vitamin A from 10 -1 to 10 -8 mg.ml-1.

Results :
We can just observe concentrations higher than 10-3mg.ml-1.