|
|
| (36 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| − | {{TCU_Taiwan/CSS}}
| |
| − | <html>
| |
| − | <style type="text/css">
| |
| − | body{
| |
| − | background-image:url(https://static.igem.org/mediawiki/2015/1/11/Tcu_taiwan2015_Igem_background.jpg);
| |
| − | background-repeat:no-repeat;
| |
| − | background-attachment:fixed;
| |
| − | background-size: 100% 100%;
| |
| − | }
| |
| | | | |
| − | #form {
| |
| − | position:static;
| |
| − | margin-left:10%;
| |
| − | margin-top:2%;
| |
| − | width:80%;
| |
| − | height:50%;
| |
| − | z-index:1;
| |
| − | overflow:visible;
| |
| − | margin-bottom:2%;
| |
| − | display: inline-block
| |
| − | }
| |
| − | </style>
| |
| − | <body>
| |
| − | <br><br><br><br><br><br>
| |
| − | <div id="form" style="background: rgba(100%,100%,100%,0.5); overflow-x:hidden;overflow-y:hidden; ">
| |
| − | <h1>
| |
| − | <table width="97%" align="center">
| |
| − | <tr><br>
| |
| − | <td align="center">
| |
| − | <span style="font-family:AR DESTINE,AR BONNIE,Comic Sans MS;line-height: 150%;">
| |
| − | <font size="8"><font-weight: 900;>
| |
| − | <br>AMP. <I>coli</I>
| |
| − | </font>
| |
| − | </span>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </table>
| |
| − |
| |
| − | <table width="97%" align="center">
| |
| − | <tr>
| |
| − | <td>
| |
| − | <p align="justify" ><span style="font-family:Levenim MT;line-height: 150%;"><font size="4">
| |
| − | AMPs have an extensive ability in disinfect. Unlike antibiotics, AMPs use chargeability puncture the cell membrane to kill the bacteria therefore by passing bacterial antibiotic drug resistance mechanisms.[1] Two kinds of AMPs were selected as our reagents: Epinecidin-1 and Signiferin.
| |
| − | <br>
| |
| − | Epinecidin-1 is a peptide comes from <I>Epinephelus coioides</I>, and Signiferin is comes from <I>Crinia signifera</I>. Both of them are extracted from the skin mucus. In addition, epinecidin-1 has the ability to help wounds healing and has been proven by animal studies.[2] Moreover, signiferin have great ability in disinfect Methicillin-Resistant <I>Staphylococcus aureus</I> (<I>S. aureus</I>), and had already been kindly proved by the TU-Delft 2013 iGEM team. [3]Combining these two properties, we believe that can alleviate the serious problem of skin injury.
| |
| − | <br>
| |
| − |
| |
| − | To produce AMPs and control AMPs expression, we apply the Lac operon and ligate the DNA of signal peptide into E. <I>coli</I> to help AMPs secret into culture medium. [4][5]Next, to prove that AMPs have the extensive ability in disinfection and helps the wound healing, selected cells and bacteria were tested <I>in vitro</I>, including the squamous epithelial cell and endothelial cell of the blood vessel and MRSA, and mice were used <I>in vivo</I>. Ultimately, create a wound dressing based on the above procedure.
| |
| − | <br>
| |
| − |
| |
| − | An excellent dressing made of AMPs will make a fast recovery.
| |
| − | <br>
| |
| − | </font>
| |
| − | </span>
| |
| − | </p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </table>
| |
| − | </h1>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div id="form" style="background: rgba(100%,100%,100%,0.5); overflow-x:hidden;overflow-y:hidden; ">
| |
| − | <p align="center">
| |
| − | <img align="middle" src="https://static.igem.org/mediawiki/2015/f/f0/Tcu_Taiwan_Project_overview.jpg" width=100% height=100% >
| |
| − | </p></div>
| |
| − |
| |
| − |
| |
| − | <div id="form" style="background: rgba(100%,100%,100%,0.5); overflow-x:hidden;overflow-y:hidden; ">
| |
| − | <table width="97%" align="center">
| |
| − | <tr>
| |
| − | <td>
| |
| − | <span style="font-family:Levenim MT;line-height: 150%;"><font size="4">
| |
| − |
| |
| − |
| |
| − | <p style="font-size:0.7cm" align="justify">
| |
| − | <a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Our_Design">
| |
| − | Antimicrobial peptide
| |
| − | </a>
| |
| − | </p>
| |
| − |
| |
| − |
| |
| − | <p align="justify"> • Epinecidin-1:</p>
| |
| − |
| |
| − | <p align="justify"> 1. From the skin mucus of <I>Epinephelus coioides</I> a kind of fish.
| |
| − | </p>
| |
| − | <p align="justify">
| |
| − | 2. Has function of killing bacteria.
| |
| − | </p>
| |
| − | <p align="justify">
| |
| − | 3. In addition, it has the ability to help wounds healing and has been proven by animal studies.
| |
| − | </p>
| |
| − | <br>
| |
| − | <p align="justify"> • Signiferin:
| |
| − | </p>
| |
| − | <p align="justify">
| |
| − | 1. From the skin mucus of <I>Crinia signifera</I> a kind of tree frog.
| |
| − | </p>
| |
| − | <p align="justify">
| |
| − | 2. Have function of killing bacteria.
| |
| − | </p>
| |
| − | <p align="justify">
| |
| − | 3. Have great ability in disinfect Methicillin-Resistant <I>Staphylococcus aureus</I> (MRSA).
| |
| − | </p>
| |
| − | <p align="justify">
| |
| − | 4. Had already been kindly proved by the 2013 TU-Delft iGEM team.
| |
| − | </p>
| |
| − | <br>
| |
| − |
| |
| − | <p style="font-size:0.7cm" align="justify">
| |
| − | <a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Experimental"> Signal peptide: </a>
| |
| − | </p>
| |
| − | <p align="justify">
| |
| − | 1. Helps AMPs to secret out of E. coli.
| |
| − | </p>
| |
| − | <p align="justify">
| |
| − | 2. From <I>Streptomyces lividans</I> to trasport chitinase C to secretion system, which has been proven to work in E.<I>coli</I>
| |
| − | </p>
| |
| − | <p align="justify">
| |
| − | by reference.
| |
| − | </p>
| |
| − | <br>
| |
| − |
| |
| − | <p style="font-size:0.7cm" align="justify">
| |
| − | <a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Reference"> Wound dressing:</a>
| |
| − | </p>
| |
| − |
| |
| − | <p align="justify">
| |
| − | Based on AMPs to develop into a potential material of wound dressing.
| |
| − | </p>
| |
| − |
| |
| − |
| |
| − | </font>
| |
| − | </span>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </table>
| |
| − | </div>
| |
| − | <div id="form" style="background: rgba(100%,100%,100%,0.5); overflow-x:hidden;overflow-y:hidden; ">
| |
| − |
| |
| − | <p style="font-size:1.2cm" align="center">
| |
| − | Reference
| |
| − | </p>
| |
| − |
| |
| − |
| |
| − | <p style="font-size:0.6cm" align="justify">
| |
| − | [1] Michael R. Yeaman & Nannette Y. Yount,..(2003) 'Mechanisms of Antimicrobial Peptide Action and Resistance' , Pharmacological Reviews,vol 55,p:27-55
| |
| − | <br><br>
| |
| − | [2] Han-Ning Huang, Chang-Jer Wu, Jyh-Yih Chen, Venugopal Rajanbabu , Chieh-Yu Pan, Yi-Lin Chan. Use of the antimicrobial peptide Epinecidin-1 to protect against MRSA infection in mice with skin injuries. Biomaterials 34 (2013) 10319e10327.
| |
| − | <br><br>
| |
| − | [3] V.M. Maselli, D. Bilusich, et al., Host-defence skin peptides of the Australian Streambank Froglet Crinia riparia: isolation and sequence determination by positive and negative ion electrospray mass spectrometry, Rapid Communications in Mass Spectrometry, Volume 20, Issue 5, pages 797–803, Mar 2006.
| |
| − | <br><br>
| |
| − | [4] Ken Tokuyasu, Satoshi Kaneko, Kiyoshi Hayashi, Yutaka Mori.Production of a recombinant chitin deacetylase in the culture medium of Escherichia coli cells.National Food Research Institute, Kannondai 2-1-2, Tsukuba 305-8642, Japan Received 26 July 1999
| |
| − | <br><br>
| |
| − | [5] TAKESHI FUJII, KIYOTAKA MIYASHITA. Multiple domain structure in a chitinase gene (chic) of Streptomyces lividans. Journal of General Microbiology (1993), 139, 677-686
| |
| − | <br><br>
| |
| − | </p>
| |
| − | <br><br><br><br><br>
| |
| − | </div>
| |
| − | <br><br><br><br><br>
| |
| − | </body>
| |
| − | </html>
| |
| − | {{TCU_Taiwan/CSSfooter}}
| |