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| − | {{TCU_Taiwan/CSS}}
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| − | background-image:url(https://static.igem.org/mediawiki/2015/1/11/Tcu_taiwan2015_Igem_background.jpg);
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| − | </style>
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| − | <body>
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| − | <br><br><br><br><br><br>
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| − | <div id="form" style="background: rgba(100%,100%,100%,0.5); overflow-x:hidden;overflow-y:hidden; ">
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| − | <h1>
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| − | <span style="font-family:Arial Black;;line-height: 150%;">
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| − | <font size="8"><font-weight: 900;>
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| − | <br>AMP. <I>coli</I>
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| − | <p align="justify" ><span style="font-family:Calibri;line-height: 150%;"><font size="5">
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| − | AMPs have an extensive ability in disinfect. Unlike antibiotics, AMPs use chargeability puncture the cell membrane to kill the bacteria therefore by passing bacterial antibiotic drug resistance mechanisms.<a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Overview#tcu_references_1">[1]</a> Two kinds of AMPs were selected as our reagents: Epinecidin-1 and Signiferin.
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| − | Epinecidin-1 is a peptide comes from <I>Epinephelus coioides</I>, and Signiferin is comes from <I>Crinia signifera</I>. Both of them are extracted from the skin mucus. In addition, epinecidin-1 has the ability to help wounds healing and has been proven by animal studies.<a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Overview#tcu_references_2">[2]</a> Moreover, signiferin have great ability in disinfect Methicillin-Resistant <I>Staphylococcus aureus</I> (<I>S. aureus</I>), and had already been kindly proved by the TU-Delft 2013 iGEM team. <a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Overview#tcu_references_3">[3]</a>Combining these two properties, we believe that can alleviate the serious problem of skin injury.
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| − | To produce AMPs and control AMPs expression, we apply the Lac operon and ligate the DNA of signal peptide into E. <I>coli</I> to help AMPs secret into culture medium. <a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Overview#tcu_references_4">[4][5]</a>Next, to prove that AMPs have the extensive ability in disinfection and helps the wound healing, selected cells and bacteria were tested <I>in vitro</I>, including the squamous epithelial cell and endothelial cell of the blood vessel and MRSA, and mice were used <I>in vivo</I>. Ultimately, create a wound dressing based on the above procedure.
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| − | An excellent dressing made of AMPs will make a fast recovery.
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| − | </h1>
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| − | </div>
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| − | <div id="form" style="background: rgba(100%,100%,100%,0.5); overflow-x:hidden;overflow-y:hidden; ">
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| − | <p align="center">
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| − | <img align="middle" src="https://static.igem.org/mediawiki/2015/f/f0/Tcu_Taiwan_Project_overview.jpg" width=100% height=100% >
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| − | </p></div>
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| − | <div id="form" style="background: rgba(100%,100%,100%,0.5); overflow-x:hidden;overflow-y:hidden; ">
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| − | <table width="97%" align="center">
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| − | <tr>
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| − | <td width="3%"></td>
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| − | <td>
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| − | <span style="font-family:Calibri;line-height: 150%;text-align:justify;"><font size="5">
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| − | <p>
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| − | <a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Our_Design">
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| − | Antimicrobial peptide
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| − | </a>
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| − | </p>
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| − | <p align="justify">• Epinecidin-1:</p>
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| − | <p align="justify">1. From the skin mucus of <I>Epinephelus coioides</I> a kind of fish.
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| − | </p>
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| − | 2. Has function of killing bacteria.
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| − | </p>
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| − | 3. In addition, it has the ability to help wounds healing and has been proven by animal studies.
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| − | </p>
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| − | <br>
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| − | <p align="justify">• Signiferin:
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| − | </p>
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| − | <p align="justify">
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| − | 1. From the skin mucus of <I>Crinia signifera</I> a kind of tree frog.
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| − | </p>
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| − | 2. Have function of killing bacteria.
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| − | </p>
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| − | 3. Have great ability in disinfect Methicillin-Resistant <I>Staphylococcus aureus</I> (MRSA).
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| − | </p>
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| − | <p align="justify">
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| − | 4. Had already been kindly proved by the 2013 TU-Delft iGEM team.
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| − | </p>
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| − | <br>
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| − | <p>
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| − | <a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Experimental">Signal peptide: </a>
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| − | </p>
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| − | 1. Helps AMPs to secret out of E. coli.
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| − | </p>
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| − | <p align="justify">
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| − | 2. From <I>Streptomyces lividans</I> to trasport chitinase C to secretion system, which has been proven to work in E.<I>coli</I>
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| − | by reference.
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| − | <p style="font-size:0.7cm" align="justify">
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| − | <a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Reference">Wound dressing:</a>
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| − | Based on AMPs to develop into a potential material of wound dressing.
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| − | </tr>
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| − | </table>
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| − | </div>
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| − | <a name="tcu_references_1"></a>
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| − | <div id="form" style="background: rgba(100%,100%,100%,0.5); overflow-x:hidden;overflow-y:hidden; ">
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| − | <table width="97%" align="center">
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| − | <p style="font-size:1.2cm" align="center">
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| − | References
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| − | </p>
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| − | <a name="tcu_references_2"></a>
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| − | [1] Michael R. Yeaman & Nannette Y. Yount,..(2003) 'Mechanisms of Antimicrobial Peptide Action and Resistance' , Pharmacological Reviews,vol 55,p:27-55
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| − | <br><br>
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| − | <a name="tcu_references_3"></a>
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| − | [2] Han-Ning Huang, Chang-Jer Wu, Jyh-Yih Chen, Venugopal Rajanbabu , Chieh-Yu Pan, Yi-Lin Chan. Use of the antimicrobial peptide Epinecidin-1 to protect against MRSA infection in mice with skin injuries. Biomaterials 34 (2013) 10319e10327.
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| − | <br><br>
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| − | <a name="tcu_references_4"></a>
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| − | [3] V.M. Maselli, D. Bilusich, et al., Host-defence skin peptides of the Australian Streambank Froglet Crinia riparia: isolation and sequence determination by positive and negative ion electrospray mass spectrometry, Rapid Communications in Mass Spectrometry, Volume 20, Issue 5, pages 797–803, Mar 2006.
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| − | <br><br>
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| − | <a name="tcu_references_5"></a>
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| − | [4] Ken Tokuyasu, Satoshi Kaneko, Kiyoshi Hayashi, Yutaka Mori.Production of a recombinant chitin deacetylase in the culture medium of Escherichia coli cells.National Food Research Institute, Kannondai<br>2-1-2, Tsukuba 305-8642, Japan Received 26 July 1999
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| − | <br><br>
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| − | [5] TAKESHI FUJII, KIYOTAKA MIYASHITA. Multiple domain structure in a chitinase gene (chic) of Streptomyces lividans. Journal of General Microbiology (1993), 139, 677-686
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| − | <br><br><br><br><br>
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| − | </body>
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| − | </html>
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| − | {{TCU_Taiwan/CSSfooter}}
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