Difference between revisions of "Team:Bordeaux/Description"
Line 59: | Line 59: | ||
<div class="col-lg-6"> | <div class="col-lg-6"> | ||
<br> | <br> | ||
− | <p align="justify" style="text-indent: 3vw;"> Before starting the project, we took a few weeks to decide which host organism we would use and how they could be useful. To begin with we looked at three different organisms: <i> Escherichia coli </i>, <i> Bacillus subtilis </i> and <i> Saccharomyces cerevisiae </i> and compared their glucan metabolic pathways. We rapidly eliminated <i> Bacillus subtilis </i> from our possible hosts due to it's lack of enzymes involved in the metabolic pathway of beta 1,3 glucans. However, we found that | + | <p align="justify" style="text-indent: 3vw;"> Before starting the project, we took a few weeks to decide <b>which host organism we would use</b> and how they could be useful. To begin with we looked at three different organisms: <i> Escherichia coli </i>, <i> Bacillus subtilis </i> and <i> Saccharomyces cerevisiae </i> and compared their glucan metabolic pathways. We rapidly eliminated <i> Bacillus subtilis </i> from our possible hosts due to it's lack of enzymes involved in the metabolic pathway of beta 1,3 glucans. However, we found that <b><i>Saccharomyces cerevisiae</i> naturally produces Curdlan</b> in it's cell wall, like <i>Agrobacterium</i>. Furthermore, <b><i> Escherichia coli </i> is only missing one enzyme</b> (the <b>Beta Glucan synthase</b>) to synthethize Curdlan. We therefore concluded that we could keep these two organisms: one where we would overexpress the beta 1,3 glucan synthase using a constititive promoter and one where we would insert the ability to create curdlan by adding the enzyme that is needed. </p> |
− | + | ||
</div> | </div> | ||
Revision as of 19:12, 4 September 2015