Difference between revisions of "Team:Birkbeck/Results"

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<h1>Under Construction</h1>
 
<h1>Under Construction</h1>
 
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<p><b><u>Fig. 1: Growth Curve of <i>E. coli</i> DH5α Strains Following Culture Optical Density of 600 nm</u></b>.</p>
 
<p><b><u>Fig. 1: Growth Curve of <i>E. coli</i> DH5α Strains Following Culture Optical Density of 600 nm</u></b>.</p>
 
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<!--<p>Growth kinetics was initially studied using 50 mL cultures. Fig. 1 shows the growth kinetics of E. coli DH5α & derivative strains containing plasmids from the <a href="https://2015.igem.org/Team:Birkbeck/InterLab_Study">InterLab</a> study.  The growth curve shows that the <i>E. coli</i> strain that contains the <i>gfp</i> expression device P1 grows at a slower rate than the other strains investigated. At 220 minutes the <i>E. coli</i> DH5α P1 strain has a significantly lower OD<sup>600</sub> than the <i>E. coli</i> DH5α (P=0.023). <i>E. coli</i> DH5α remains very highly significantly higher in OD600 than E. coli DH5α with the P1-gfp expression device (P=<0.001). The only difference between the E. coli DH5α & E. coli DH5α positive control device is observed at 280 minutes into the growth curve (P=0.016) where the positive control has a higher OD<sub>600</sub>. The multiple comparison table showing P values can be viewed in <a href="https://2015.igem.org/Team:Birkbeck/Results/Table_S1"></a><b>Table S1</b></p>-->
 
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<p><b><u>Fig. 2: Growth Curve of <i>E. coli</i> DH5α Strains Following Culture Optical Density of 395 nm</u></b>.</p>
 
<p><b><u>Fig. 2: Growth Curve of <i>E. coli</i> DH5α Strains Following Culture Optical Density of 395 nm</u></b>.</p>
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Revision as of 23:04, 5 September 2015

iGEM Westminster Logo




Birkbeck iGEM

The Owligos are the first-ever team entered into the international Genetically Engineered Machine (iGEM) Competition by Birkbeck, University of London. We’re a varied group of students who reflect the diversity and unique character of our institution: many of us have chosen science as a second career, having already spent some time in full-time work. For most of us, this has meant making our way through a degree while continuing to work full-time. Hopefully this kind of dedication will help us successfully navigate our way through our iGEM project.

Project Aim

Our project aims to create a new diagnostic solution that will be low-tech and cost-effective enough to allow its usage in deprived and remote communities. We’re attempting to engineer a bacteriophage lambda chassis to change its host affinity, while simultaneously adding a marker that will facilitate easy detection of a target bacterial pathogen in patient samples.

To demonstrate this approach as a proof of concept for the competition, we plan to change this affinity between different strains of E.coli; however, ultimately we hope to demonstrate that this principle could also be applied to alter the phage’s host range to other bacterial species. We could then provide a modular system capable of diagnosing a range of diseases. Of course, we haven’t chosen a simple goal. But as Birkbeck pioneers, we are determined to prove ourselves by making our project a success. We can’t wait to present the results of our work at the Giant Jamboree in September!




Under Construction

Fig. 1: Growth Curve of E. coli DH5α Strains Following Culture Optical Density of 600 nm.


Fig. 2: Growth Curve of E. coli DH5α Strains Following Culture Optical Density of 395 nm.



Fig. 3: Viable Count of E. coli DH5α After 60 mins.


Fig. 4: Viable Count of E. coli DH5α After 175 mins.


Fig. 5: Growth Curves of Different Strains of E. coli DH5α Following Culture Optical Density at 601 nm.


Fig. 6: Growth Curves of Different Strains of E. coli DH5α Following Culture Optical Density at 501 nm.


Fig. 7: Growth Curves of Different Strains of E. coli DH5α Following Culture Optical Density at 475 nm.


Fig. 8: Growth Curves of Different Strains of E. coli DH5α Following Culture Optical Density at 395 nm.


Fig. 9: Growth Curves of Different Strains of E. coli DH5α Following Culture Fluorescence.