Difference between revisions of "Team:Birkbeck/Results"

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<caption class="title" style="width:522px">Descriptive Statistics of 1 hour Viable Count.<span class="details"></span></caption>
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<caption class="title" style="width:522px">Descriptive Statistics of 1 hour Viable Count of <i>E. coli</i> DH5α.<span class="details"></span></caption>
 
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<p><b><u>Table 1: Descriptive Statistics of 60 minutes Viable Count</u></b>.</p>
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<p><b><u>Table 1: Descriptive Statistics of 60 minutes Viable Count of <i>E. coli</i> DH5α.</u></b>.</p>
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<center><img src="https://static.igem.org/mediawiki/2015/e/e6/Viable_count_of_DH5_alpha_after_175_mins_team_birkbeck_iGEM_2015.jpg"></center>
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<img src="https://static.igem.org/mediawiki/2015/e/e6/Viable_count_of_DH5_alpha_after_175_mins_team_birkbeck_iGEM_2015.jpg">
 
<p><b><u>Fig. 4: Viable Count of <i>E. coli</i> DH5α After 175 mins</u></b>.</p>
 
<p><b><u>Fig. 4: Viable Count of <i>E. coli</i> DH5α After 175 mins</u></b>.</p>
 
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<caption class="title" style="width:547px"><span class="details">Descriptive Statistics of 175 Minutes Viable Count of <i>E. coli</i> DH5α.</span></caption>
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<td class=" e dataAreaTop vCC">90000000</td>
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<td class=" e dataAreaTop vCC">175000000</td>
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<td class=" e dataAreaTop vCC">133333333.33</td>
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<td class=" e dataAreaTop vCC">29154759.474</td>
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<!--End of the descriptive statistics table for 175 minutes of growth-->
 
<!--End of the descriptive statistics table for 175 minutes of growth-->
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<p><b><u>Table 2: Descriptive Statistics of 175 minutes Viable Count of <i>E. coli</i> DH5α.</u></b></p>
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<br>
 
<center><IMG SRC="https://static.igem.org/mediawiki/2015/8/8a/Growth_curve_601_nm_96-microtitre_Team_birkbeck_iGEM_2015_data.jpg "></center>
 
<center><IMG SRC="https://static.igem.org/mediawiki/2015/8/8a/Growth_curve_601_nm_96-microtitre_Team_birkbeck_iGEM_2015_data.jpg "></center>
 
<p><b><u>Fig. 5: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Optical Density at 601 nm</u></b>.</p>
 
<p><b><u>Fig. 5: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Optical Density at 601 nm</u></b>.</p>

Revision as of 20:19, 6 September 2015

iGEM Westminster Logo




Birkbeck iGEM

The Owligos are the first-ever team entered into the international Genetically Engineered Machine (iGEM) Competition by Birkbeck, University of London. We’re a varied group of students who reflect the diversity and unique character of our institution: many of us have chosen science as a second career, having already spent some time in full-time work. For most of us, this has meant making our way through a degree while continuing to work full-time. Hopefully this kind of dedication will help us successfully navigate our way through our iGEM project.

Project Aim

Our project aims to create a new diagnostic solution that will be low-tech and cost-effective enough to allow its usage in deprived and remote communities. We’re attempting to engineer a bacteriophage lambda chassis to change its host affinity, while simultaneously adding a marker that will facilitate easy detection of a target bacterial pathogen in patient samples.

To demonstrate this approach as a proof of concept for the competition, we plan to change this affinity between different strains of E.coli; however, ultimately we hope to demonstrate that this principle could also be applied to alter the phage’s host range to other bacterial species. We could then provide a modular system capable of diagnosing a range of diseases. Of course, we haven’t chosen a simple goal. But as Birkbeck pioneers, we are determined to prove ourselves by making our project a success. We can’t wait to present the results of our work at the Giant Jamboree in September!




Under Construction

Fig. 1: Growth Curve of E. coli DH5α Strains Following Culture Optical Density of 600 nm.


Fig. 2: Growth Curve of E. coli DH5α Strains Following Culture Optical Density of 395 nm.



Fig. 3: Viable Count of E. coli DH5α After 60 mins.


Descriptive Statistics of 1 hour Viable Count of E. coli DH5α.
  N Minimum Maximum Mean Std. Deviation
Viable.Count 9 1600000 3750000 2566666.67 627495.020
Valid N (listwise) 9        

Table 1: Descriptive Statistics of 60 minutes Viable Count of E. coli DH5α..

Fig. 4: Viable Count of E. coli DH5α After 175 mins.


Descriptive Statistics of 175 Minutes Viable Count of E. coli DH5α.
  N Minimum Maximum Mean Std. Deviation
Viable.Count 9 90000000 175000000 133333333.33 29154759.474
Valid N (listwise) 9        

Table 2: Descriptive Statistics of 175 minutes Viable Count of E. coli DH5α.


Fig. 5: Growth Curves of Different Strains of E. coli DH5α Following Culture Optical Density at 601 nm.


Fig. 6: Growth Curves of Different Strains of E. coli DH5α Following Culture Optical Density at 501 nm.


Fig. 7: Growth Curves of Different Strains of E. coli DH5α Following Culture Optical Density at 475 nm.


Fig. 8: Growth Curves of Different Strains of E. coli DH5α Following Culture Optical Density at 395 nm.


Fig. 9: Growth Curves of Different Strains of E. coli DH5α Following Culture Fluorescence.