Difference between revisions of "Team:Shiyan SY China/Project.html"

  With increased agriculture activities around the world, it becomes a common practice to use pesticides to manage pest problem. Runoff can carry field pesticide into aquatic environment while wind can carry them to other fields, potentially affecting other species. Over time, repeated application increases pest resistance and facilitate the pest resurgence. Further, especially in China, toxic pesticide residues on green vegetable and fruits become a major public health problem.

  In order to provide a solution, we design an engineered bacterium, secreting the OMP enzyme to degrade the common toxic pesticide residues. Its secretion is under the temperature control and can only be activated at specified temperature. To avoid the secondary pollution, a UV-induced suicide gene is inserted into the bacteria: upon exposure to UV or sunshine, the suicide procedure is induced. This purpose of the design is to remove toxic pesticide safely without affecting the environment.

  In our project to build biological modules, reference the study design of this project comes from the igem previous years, a large number of information and information from other research teams on the rest of society, the information in this project, coordinated and integrated to form and project implementation content.

  1) a strong promoter sequence used in this project, quoted from igem of BB_J23106.

  2) RNA thermometer sequences used in this project, quoted from igem of BB_K115017.

  3) organophosphorus pesticides used in this project degrading enzyme gene sequence opdA, quoted from igem of BB_K215090, BB_K215091 and BB_K1010008.

  4) The project used an inducible promoter repair enzyme RecA (SOS) sequence, quoted from igem of BB_J22106.

  5) ccdB suicide gene sequences used in this project, quoted from igem of BB_K145151 and BB_K1010007.

  6) ompA signal peptide sequence used in this project, quoted from GenBank sequences numbered AJ617284.1.

  We connect we the World magazine and publish the Igem monograph . In the magazine we discuss the safety, ethic, advantage and disadvantage of the synthetic biology. We record the process of our team, including the safety of the experiment and the what we learned during the experiment in the CAS. World magazine is established by the Liaoning province Shiyan high school’s international department., facing the entire school and the high school in the province,; therefore, through the magazine, we are able to share and discuss the igem with other students.

  The strong promoter has a strong function to start the downstream RNA transcription, and the transcription of the downstream opdA gene promoter is regulated by the promoter. The RNA structure of the thermometer RNA, which is formed by the RNA structure of the sequence, is formed at the temperature below 32. The structure of the RNA is blocked by the RNA. OmpA signal peptide can lead to opdA protein molecules secreted by the downstream expression of the host cell wall, and in the cell wall to form a opdA enzyme with the degradation of organic phosphorus pesticides, the enzyme can be hydrolyzed in the environment of organic phosphorus pesticides, pesticide toxicity.

  We do a new way spread the knowledge of biology to the public, and we update the dynamic of our team work.