Difference between revisions of "Team:KU Leuven/InterLabStudy"

(Undo revision 180809 by VinnieVD (talk))
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<link rel="icon" href="https://static.igem.org/mediawiki/2015/9/9c/Ku_Leuven_Favicon.gif" />
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<link rel="shortcut icon" href="https://static.igem.org/mediawiki/2015/9/9c/Ku_Leuven_Favicon.gif" /
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}
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h2{
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text-allign="center";
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text-transform: none;
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b1{
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font-size:0.85em;
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margin-top: 10px;
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#tab1 { display:inline-block;
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      margin-left: 40px; }
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#tab2 { display:inline-block;
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#tab3 { display:inline-block;
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.tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:#aaa;color:#333;background-color:#fff;}
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.tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:#aaa;color:#fff;background-color:#f38630;} <!- change colour
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.tg .tg-s6z2{text-align:center}
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</style>
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<link rel="stylesheet" type="text/css"
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href="https://2015.igem.org/Template:KU_Leuven/Lightbox/CSS?action=raw&ctype=text/css" />
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</head>
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<script type="text/javascript" src="http://yourjavascript.com/11518521655/lightbox.js"></script>
  
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<body>
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<div id="summarywrapper">
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<div class="summaryheader">
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<div id="logowrapper">
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  <div class="summaryimg">
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<div id="logoiGEMKUL">
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  <img src="https://static.igem.org/mediawiki/2015/b/b7/KU_Leuven_Researchbanner.jpg" width="100%">
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  <img src="https://static.igem.org/mediawiki/2015/2/23/Team_KU_Leuven_banner.jpg"  alt="Logo" width="100%" >
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  <div class="head">
 +
    <h2> Interlab study </h2>
 +
  </div>
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</div>
 
</div>
 
</div>
  
  
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<div id="center">
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<!------------------------------------------------------Begin Content--------------------------------------------------------->
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<div id="pagetitle">
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<div class="summarytext1">
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<h2>Research</h2>
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<div class="part">
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</div>
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<div id="scrolldown"  class="center">
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<img src="https://static.igem.org/mediawiki/2015/1/1d/KU_Leuven_Arrows_wiki.png" alt="Scroll down" width="100%">
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</div>
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</div>
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−
<div id="summarylogos">
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<h2>Results</h2>
 +
<p>
 +
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
 +
</p>
 +
<br>
 +
<p>
 +
<b>Table 1</b>
 +
Fluorescence measurements
 +
</p>
 +
</br>
 +
<div class="tg-wrap" style="width:100%"><table class="tg" style="width:100%">
 +
  <tr>
 +
    <th class="tg-031e">LB + Cam</th>
 +
    <th class="tg-031e">LB+Cam+Cells</th>
 +
    <th class="tg-s6z2" colspan="3">D1</th>
 +
    <th class="tg-s6z2" colspan="3">D2</th>
 +
    <th class="tg-s6z2" colspan="3">D3</th>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">512</td>
 +
    <td class="tg-031e">601</td>
 +
    <td class="tg-031e">737</td>
 +
    <td class="tg-031e">685</td>
 +
    <td class="tg-031e">751</td>
 +
    <td class="tg-031e">9425</td>
 +
    <td class="tg-031e">9322</td>
 +
    <td class="tg-031e">9737</td>
 +
    <td class="tg-031e">22786</td>
 +
    <td class="tg-031e">25895</td>
 +
    <td class="tg-031e">25048</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">546</td>
 +
    <td class="tg-031e">594</td>
 +
    <td class="tg-031e">754</td>
 +
    <td class="tg-031e">641</td>
 +
    <td class="tg-031e">641</td>
 +
    <td class="tg-031e">9669</td>
 +
    <td class="tg-031e">9545</td>
 +
    <td class="tg-031e">9876</td>
 +
    <td class="tg-031e">23159</td>
 +
    <td class="tg-031e">26460</td>
 +
    <td class="tg-031e">24785</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">549</td>
 +
    <td class="tg-031e">587</td>
 +
    <td class="tg-031e">722</td>
 +
    <td class="tg-031e">660</td>
 +
    <td class="tg-031e">697</td>
 +
    <td class="tg-031e">9407</td>
 +
    <td class="tg-031e">9321</td>
 +
    <td class="tg-031e">9677</td>
 +
    <td class="tg-031e">22719</td>
 +
    <td class="tg-031e">25931</td>
 +
    <td class="tg-031e">24935</td>
 +
  </tr>
 +
<p>D1, D2, D3  <span id="tab1">Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively</span></p>
 +
<br>
 +
</table></div>
  
−
<div id="summarylogotwitter">
+
<p>
−
<a href="https://twitter.com/kuleuven_igem" height="100%"><img src="https://static.igem.org/mediawiki/2015/b/b8/KU_Leuven_Twitter-logo.png" alt="Twitter" width="100%"></a>
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
−
</div>
+
</p>
 +
<br>
  
−
<div id="summarylogofacebook">
 
−
<a href="https://www.facebook.com/KULeuveniGEM?fref=ts" alt="Facebook" height="50px"><img src="https://static.igem.org/mediawiki/2015/d/df/KU_Leuven_Fblogo.png" alt="mail" width="100%"></a> 
 
−
</div>
 
−
 
−
<div id="summarylogomail">
 
−
<a href="mailto:igem@chem.kuleuven.be"><img src="https://static.igem.org/mediawiki/2015/1/11/KU_Leuven_mail.png" alt="mail" width="100%"></a> 
 
−
</div>
 
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</div>
 
−
</div>
 
−
<div class="summary">
 
 
<p>
 
<p>
−
We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation and tissue regeneration.
+
<b>Table 2 </b> Absorbance measurements
 
</p>
 
</p>
−
<br/>
+
</br>
−
<br/>
+
−
<br/>
+
−
<br/>
+
−
</div>
+
−
</div>
+
  
−
<div class="subsections">
+
<div class="tg-wrap" style="width:100%"><table class="tg" style="width:100%" >
−
<div class="subsectionwrapper">
+
  <tr>
−
<div class="subimgrow">
+
    <th class="tg-031e">LB + Cam</th>
 +
    <th class="tg-031e">LB+Cam+Cells</th>
 +
    <th class="tg-s6z2" colspan="3">D1</th>
 +
    <th class="tg-s6z2" colspan="3">D2</th>
 +
    <th class="tg-s6z2" colspan="3">D3</th>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">0.0434</td>
 +
    <td class="tg-031e">0.2052</td>
 +
    <td class="tg-031e">0.2174</td>
 +
    <td class="tg-031e">0.1942</td>
 +
    <td class="tg-031e">0.2201</td>
 +
    <td class="tg-031e">0.2088</td>
 +
    <td class="tg-031e">0.2082</td>
 +
    <td class="tg-031e">0.2154</td>
 +
    <td class="tg-031e">0.2139</td>
 +
    <td class="tg-031e">0.2210</td>
 +
    <td class="tg-031e">0.2218</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">0.0405</td>
 +
    <td class="tg-031e">0.2000</td>
 +
    <td class="tg-031e">0.2182</td>
 +
    <td class="tg-031e">0.1974</td>
 +
    <td class="tg-031e">0.1980</td>
 +
    <td class="tg-031e">0.2108</td>
 +
    <td class="tg-031e">0.2111</td>
 +
    <td class="tg-031e">0.2123</td>
 +
    <td class="tg-031e">0.2143</td>
 +
    <td class="tg-031e">0.2179</td>
 +
    <td class="tg-031e">0.2056</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">0.0379</td>
 +
    <td class="tg-031e">0.2064</td>
 +
    <td class="tg-031e">0.2141</td>
 +
    <td class="tg-031e">0.1961</td>
 +
    <td class="tg-031e">0.2036</td>
 +
    <td class="tg-031e">0.2137</td>
 +
    <td class="tg-031e">0.2117</td>
 +
    <td class="tg-031e">0.2164</td>
 +
    <td class="tg-031e">0.2202</td>
 +
    <td class="tg-031e">0.2261</td>
 +
    <td class="tg-031e">0.2083</td>
 +
  </tr>
 +
<p>D1, D2, D3  <span id="tab1">Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively</span></p>
 +
<br>
 +
</table>
  
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<div class="subimg">
+
<p>
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Idea">
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<img src="https://static.igem.org/mediawiki/2015/4/4d/KU_Leuven_scheme_pattern_formation.png" width="95%" >
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</a>
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<img src="https://static.igem.org/mediawiki/2015/4/4e/KU_Leuven_Gblocks.jpg" width="100%">
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<img src="https://static.igem.org/mediawiki/2015/3/32/KU_Leuven_AgarPlates.jpg" width="100%">
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<div class="subimg">
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<img src="https://static.igem.org/mediawiki/2015/b/b7/KU_Leuven_Motility2.jpg" width="92.4%">
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<div class="whitespace">
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<div class="subtextrow">
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<div class="subtext">
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<a href="https://2015.igem.org/Team:KU_Leuven/Research/Idea">
+
−
<h2>Idea</h2>
+
−
<p> A detailed description about the interaction between our two cells and the genetic circuit can be found here.
+
−
<br/>
+
−
<div class="more"><p>Read more</p></div>
+
 
</p>
 
</p>
−
</a>
+
<br>
−
</div>
+
  
−
<div class="whitespace">
 
−
</div>
 
−
 
−
<div class="subtext">
 
−
<h2>Parts</h2>
 
 
<p>
 
<p>
−
More coming soon
+
<b>Table 3</b> Values for Biological replicates
 
</p>
 
</p>
−
<!---------<p>A list of all used biobricks, the modifications we performed on them and the new ones we designed.
+
</br>
−
<br/>
+
<div class="tg-wrap" style="width:100%"><table class="tg" style="width:100%">
−
<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
+
  <tr>
−
</p>------->
+
    <th class="tg-031e"></th>
−
</div>
+
    <th class="tg-s6z2" colspan="3">D1</th>
−
 
+
    <th class="tg-s6z2" colspan="3">D2</th>
−
<div class="whitespace">
+
    <th class="tg-s6z2" colspan="3">D3</th>
−
</div>
+
    <th class="tg-s6z2">LB+Cam+Cells</th>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e"></td>
 +
    <td class="tg-031e">T1</td>
 +
    <td class="tg-031e">T2</td>
 +
    <td class="tg-031e">T3</td>
 +
    <td class="tg-031e">T1</td>
 +
    <td class="tg-031e">T2</td>
 +
    <td class="tg-031e">T3</td>
 +
    <td class="tg-031e">T1</td>
 +
    <td class="tg-031e">T2</td>
 +
    <td class="tg-031e">T2</td>
 +
    <td class="tg-031e"></td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">B1</td>
 +
    <td class="tg-031e">3390</td>
 +
    <td class="tg-031e">3456</td>
 +
    <td class="tg-031e">3372</td>
 +
    <td class="tg-031e">45139</td>
 +
    <td class="tg-031e">45868</td>
 +
    <td class="tg-031e">44020</td>
 +
    <td class="tg-031e">106526</td>
 +
    <td class="tg-031e">108068</td>
 +
    <td class="tg-031e">103174</td>
 +
    <td class="tg-031e">2929</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">B2</td>
 +
    <td class="tg-031e">3527</td>
 +
    <td class="tg-031e">3247</td>
 +
    <td class="tg-031e">3366</td>
 +
    <td class="tg-031e">44774</td>
 +
    <td class="tg-031e">45216</td>
 +
    <td class="tg-031e">44029</td>
 +
    <td class="tg-031e">117172</td>
 +
    <td class="tg-031e">121432</td>
 +
    <td class="tg-031e">114688</td>
 +
    <td class="tg-031e">2970</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">B3</td>
 +
    <td class="tg-031e">3399</td>
 +
    <td class="tg-031e">3237</td>
 +
    <td class="tg-031e">3426</td>
 +
    <td class="tg-031e">45204</td>
 +
    <td class="tg-031e">46519</td>
 +
    <td class="tg-031e">44718</td>
 +
    <td class="tg-031e">112931</td>
 +
    <td class="tg-031e">120550</td>
 +
    <td class="tg-031e">119707</td>
 +
    <td class="tg-031e">2844</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">Brep Avg</td>
 +
    <td class="tg-031e">3439</td>
 +
    <td class="tg-031e">3313</td>
 +
    <td class="tg-031e">3388</td>
 +
    <td class="tg-031e">45039</td>
 +
    <td class="tg-031e">45868</td>
 +
    <td class="tg-031e">44256</td>
 +
    <td class="tg-031e">112210</td>
 +
    <td class="tg-031e">116683</td>
 +
    <td class="tg-031e">112523</td>
 +
    <td class="tg-031e" rowspan="2"></td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">BRep SD</td>
 +
    <td class="tg-031e">270</td>
 +
    <td class="tg-031e">101</td>
 +
    <td class="tg-031e">162</td>
 +
    <td class="tg-031e">189</td>
 +
    <td class="tg-031e">532</td>
 +
    <td class="tg-031e">327</td>
 +
    <td class="tg-031e">4376</td>
 +
    <td class="tg-031e">6102</td>
 +
    <td class="tg-031e">6921</td>
 +
  </tr>
 +
<p>D1, D2, D3  <span id="tab1">Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively</span></p>
 +
<p>B1, B2, B3  <span id="tab2"> Biological replicates of the respective devices</span></p>
 +
<p>T1, T2,  T3  <span id="tab3"> Technical replicates</span></p>
 +
</br>
 +
</table>
 +
<p>
 +
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
 +
</p>
 +
<br>
 +
<p>
 +
<b> Table 4 </b> Values for Technical replicates
 +
</p>
 +
</br>
 +
<table class="tg" style="width:100%">
 +
  <tr>
 +
    <th class="tg-031e"></th>
 +
    <th class="tg-s6z2" colspan="3">D1</th>
 +
    <th class="tg-s6z2" colspan="3">D2</th>
 +
    <th class="tg-s6z2" colspan="3">D3</th>
 +
    <th class="tg-031e">LB+Cam+ Cells</th>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e"></td>
 +
    <td class="tg-031e">B1</td>
 +
    <td class="tg-031e">B2</td>
 +
    <td class="tg-031e">B3</td>
 +
    <td class="tg-031e">B1</td>
 +
    <td class="tg-031e">B2</td>
 +
    <td class="tg-031e">B3</td>
 +
    <td class="tg-031e">B1</td>
 +
    <td class="tg-031e">B2</td>
 +
    <td class="tg-031e">B3</td>
 +
    <td class="tg-031e"></td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">T1</td>
 +
    <td class="tg-031e">3390</td>
 +
    <td class="tg-031e">3527</td>
 +
    <td class="tg-031e">3399</td>
 +
    <td class="tg-031e">45139</td>
 +
    <td class="tg-031e">44774</td>
 +
    <td class="tg-031e">45204</td>
 +
    <td class="tg-031e">106526</td>
 +
    <td class="tg-031e">117172</td>
 +
    <td class="tg-031e">112931</td>
 +
    <td class="tg-031e">2929</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">T2</td>
 +
    <td class="tg-031e">3456</td>
 +
    <td class="tg-031e">3247</td>
 +
    <td class="tg-031e">3237</td>
 +
    <td class="tg-031e">45868</td>
 +
    <td class="tg-031e">45216</td>
 +
    <td class="tg-031e">46519</td>
 +
    <td class="tg-031e">108068</td>
 +
    <td class="tg-031e">121432</td>
 +
    <td class="tg-031e">120550</td>
 +
    <td class="tg-031e">2970</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">T3</td>
 +
    <td class="tg-031e">3372</td>
 +
    <td class="tg-031e">3366</td>
 +
    <td class="tg-031e">3426</td>
 +
    <td class="tg-031e">44020</td>
 +
    <td class="tg-031e">44029</td>
 +
    <td class="tg-031e">44718</td>
 +
    <td class="tg-031e">103174</td>
 +
    <td class="tg-031e">114688</td>
 +
    <td class="tg-031e">119707</td>
 +
    <td class="tg-031e">2844</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">TRep Avg</td>
 +
    <td class="tg-031e">3406</td>
 +
    <td class="tg-031e">3380</td>
 +
    <td class="tg-031e">3354</td>
 +
    <td class="tg-031e">45009</td>
 +
    <td class="tg-031e">44673</td>
 +
    <td class="tg-031e">45480</td>
 +
    <td class="tg-031e">105923</td>
 +
    <td class="tg-031e">117764</td>
 +
    <td class="tg-031e">117729</td>
 +
    <td class="tg-031e">2914</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">TRep SD</td>
 +
    <td class="tg-031e">36</td>
 +
    <td class="tg-031e">115</td>
 +
    <td class="tg-031e">140</td>
 +
    <td class="tg-031e">760</td>
 +
    <td class="tg-031e">490</td>
 +
    <td class="tg-031e">761</td>
 +
    <td class="tg-031e">2043</td>
 +
    <td class="tg-031e">2785</td>
 +
    <td class="tg-031e">3410</td>
 +
    <td class="tg-031e">52</td>
 +
  </tr>
  
−
<div class="subtext">
+
<p>D1, D2, D3  <span id="tab1">Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively</span></p>
−
  <h2>Methods</h2>
+
<p>B1, B2, B3 <span id="tab2"> Biological replicates of the respective devices</span></p>
 +
<p>T1, T2,  T3  <span id="tab3"> Technical replicates</span></p>
 +
</br>
 +
</table>
 +
<div>
 
<p>
 
<p>
−
More coming soon
+
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
 
</p>
 
</p>
−
<!---<p>Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters
+
<br>
−
<br/>
+
−
<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
+
−
</p>----->
+
−
</div>
+
  
−
<div class="whitespace">
 
−
</div>
 
−
 
−
<div class="subtext">
 
−
<!------
 
−
<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">---->
 
−
<h2>Results</h2>
 
 
<p>
 
<p>
−
More coming soon
+
<b> Table 5 </b> Standard graph
 
</p>
 
</p>
−
<!--------<p>The results of our experiments will appear here.
 
−
<br/>
 
−
<div class="more"><p>
 
−
Read more</p>
 
−
</div>
 
−
</p>--------->
 
−
</a>
 
−
</div>
 
−
</div>
 
  
−
<div class="subimgrowm">
+
</br>
 +
<table class="tg" style="width:100%">
 +
  <tr>
 +
    <th class="tg-s6z2" colspan="7">Fluorescein standard</th>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">concentration (ng/mL)</td>
 +
    <td class="tg-031e">F1</td>
 +
    <td class="tg-031e">F2</td>
 +
    <td class="tg-031e">F3</td>
 +
    <td class="tg-031e">average F</td>
 +
    <td class="tg-031e">average F'</td>
 +
    <td class="tg-031e">SD</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">0</td>
 +
    <td class="tg-031e">9</td>
 +
    <td class="tg-031e">13</td>
 +
    <td class="tg-031e">12</td>
 +
    <td class="tg-031e">11</td>
 +
    <td class="tg-031e">0</td>
 +
    <td class="tg-031e">1.699673171</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">10</td>
 +
    <td class="tg-031e">1140</td>
 +
    <td class="tg-031e">1168</td>
 +
    <td class="tg-031e">1151</td>
 +
    <td class="tg-031e">1153</td>
 +
    <td class="tg-031e">1142</td>
 +
    <td class="tg-031e">11.5181017</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">25</td>
 +
    <td class="tg-031e">2543</td>
 +
    <td class="tg-031e">2600</td>
 +
    <td class="tg-031e">2631</td>
 +
    <td class="tg-031e">2591</td>
 +
    <td class="tg-031e">2580</td>
 +
    <td class="tg-031e">36.4447832</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">50</td>
 +
    <td class="tg-031e">4822</td>
 +
    <td class="tg-031e">5097</td>
 +
    <td class="tg-031e">5154</td>
 +
    <td class="tg-031e">5024</td>
 +
    <td class="tg-031e">5013</td>
 +
    <td class="tg-031e">144.9513328</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">125</td>
 +
    <td class="tg-031e">12879</td>
 +
    <td class="tg-031e">12902</td>
 +
    <td class="tg-031e">12931</td>
 +
    <td class="tg-031e">12904</td>
 +
    <td class="tg-031e">12893</td>
 +
    <td class="tg-031e">21.27596453</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">250</td>
 +
    <td class="tg-031e">25494</td>
 +
    <td class="tg-031e">25761</td>
 +
    <td class="tg-031e">25765</td>
 +
    <td class="tg-031e">25673</td>
 +
    <td class="tg-031e">25662</td>
 +
    <td class="tg-031e">126.8183303</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">375</td>
 +
    <td class="tg-031e">37474</td>
 +
    <td class="tg-031e">37505</td>
 +
    <td class="tg-031e">37899</td>
 +
    <td class="tg-031e">37626</td>
 +
    <td class="tg-031e">37615</td>
 +
    <td class="tg-031e">193.4545597</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">500</td>
 +
    <td class="tg-031e">46698</td>
 +
    <td class="tg-031e">49051</td>
 +
    <td class="tg-031e">48798</td>
 +
    <td class="tg-031e">48182</td>
 +
    <td class="tg-031e">48171</td>
 +
    <td class="tg-031e">1054.652023</td>
 +
  </tr>
  
−
<div class="subimg">
+
</table>
−
<b>Idea</b>
+
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Idea">
+
−
<img src="https://static.igem.org/mediawiki/2015/4/4d/KU_Leuven_scheme_pattern_formation.png" width="95%" >
+
−
</a>
+
−
</div>
+
  
−
<div class="whitespace">
 
−
</div>
 
  
−
<div class="subtext">
+
<div class="center">
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Idea">
+
<div id="image1">
−
<p> A detailed description about the interaction between our two cells and the genetic circuit can be found here.
+
−
</p>
+
    <img src="https://static.igem.org/mediawiki/2015/c/c2/KU_Leuven_Standardcurve.png" style="width:100%">
−
</a>
+
<h4>  
 +
<div id=figure1> <b>Figure 1</b> </div>
 +
Standard curve for fluorescein </h4>
 
</div>
 
</div>
−
 
 
</div>
 
</div>
−
<div class="subimgrowm">
 
  
−
 
−
<div class="subimg">
 
−
<b>Parts</b>
 
−
<img src="https://static.igem.org/mediawiki/2015/4/4e/KU_Leuven_Gblocks.jpg" width="100%">
 
−
</div>
 
−
 
−
<div class="whitespace">
 
−
</div>
 
−
 
−
<div class="subtext">
 
 
<p>
 
<p>
−
More coming soon
+
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
 
</p>
 
</p>
−
<!---------<p>A list of all used biobricks, the modifications we performed on them and the new ones we designed.
+
<br>
−
<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
+
<p>
−
</p>------->
+
<b> Table 6 </b> Extrapolation of GFP concentration using fluorescence measurements and fluorescein standard
−
</div>
+
</p>
 +
</br>
  
−
</div>
+
<table class="tg" style="width:100%">
−
<div class="subimgrowm">
+
  <tr>
 +
    <th class="tg-031e"></th>
 +
    <th class="tg-s6z2" colspan="3">D1</th>
 +
    <th class="tg-s6z2" colspan="3">D2</th>
 +
    <th class="tg-s6z2" colspan="3">D3</th>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">Brep Avg</td>
 +
    <td class="tg-031e">3439</td>
 +
    <td class="tg-031e">3313</td>
 +
    <td class="tg-031e">3388</td>
 +
    <td class="tg-031e">45039</td>
 +
    <td class="tg-031e">45868</td>
 +
    <td class="tg-031e">44256</td>
 +
    <td class="tg-031e">112210</td>
 +
    <td class="tg-031e">116683</td>
 +
    <td class="tg-031e">112523</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">(Brep Avg) - (LB+cam+cells avg)</td>
 +
    <td class="tg-031e">525</td>
 +
    <td class="tg-031e">399</td>
 +
    <td class="tg-031e">474</td>
 +
    <td class="tg-031e">42125</td>
 +
    <td class="tg-031e">42954</td>
 +
    <td class="tg-031e">41342</td>
 +
    <td class="tg-031e">109296</td>
 +
    <td class="tg-031e">113769</td>
 +
    <td class="tg-031e">109609</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">Concentration (ng/mL)</td>
 +
    <td class="tg-031e">1.929</td>
 +
    <td class="tg-031e">0.639</td>
 +
    <td class="tg-031e">1.407</td>
 +
    <td class="tg-031e">427.88</td>
 +
    <td class="tg-031e">436.36</td>
 +
    <td class="tg-031e">419.88</td>
 +
    <td class="tg-031e">1115.64</td>
 +
    <td class="tg-031e">1161.44</td>
 +
    <td class="tg-031e">1118.85</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">TRep Avg</td>
 +
    <td class="tg-031e">3406</td>
 +
    <td class="tg-031e">3380</td>
 +
    <td class="tg-031e">3354</td>
 +
    <td class="tg-031e">45009</td>
 +
    <td class="tg-031e">44673</td>
 +
    <td class="tg-031e">45480</td>
 +
    <td class="tg-031e">105923</td>
 +
    <td class="tg-031e">117764</td>
 +
    <td class="tg-031e">117729</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">(Trep avg) - (LB+cam+cellsavg)</td>
 +
    <td class="tg-031e">492</td>
 +
    <td class="tg-031e">466</td>
 +
    <td class="tg-031e">440</td>
 +
    <td class="tg-031e">42095</td>
 +
    <td class="tg-031e">41759</td>
 +
    <td class="tg-031e">42566</td>
 +
    <td class="tg-031e">103009</td>
 +
    <td class="tg-031e">114850</td>
 +
    <td class="tg-031e">114815</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="tg-031e">Concentration (ng/mL)</td>
 +
    <td class="tg-031e">1.591</td>
 +
    <td class="tg-031e">1.325</td>
 +
    <td class="tg-031e">1.059</td>
 +
    <td class="tg-031e">427.56</td>
 +
    <td class="tg-031e">424.12</td>
 +
    <td class="tg-031e">432.39</td>
 +
    <td class="tg-031e">1051.27</td>
 +
    <td class="tg-031e">1172.51</td>
 +
    <td class="tg-031e">1172.15</td>
 +
  </tr>
 +
</table>
  
  
−
<div class="subimg">
 
−
<b>Methods</b>
 
−
<img src="https://static.igem.org/mediawiki/2015/3/32/KU_Leuven_AgarPlates.jpg" width="100%">
 
 
</div>
 
</div>
−
<div class="whitespace">
 
 
</div>
 
</div>
  
−
<div class="subtext">
 
−
<p>
 
−
More coming soon
 
−
</p>
 
−
<!---<p>Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters
 
−
<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
 
−
</p>----->
 
−
</div>
 
  
−
</div>
+
<div class="summarytext1">
−
<div class="subimgrowm">
+
<div class="part">
 +
<h2>Protocols</h2>
  
−
<div class="subimg">
+
<p>
−
<b>Results</b>
+
−
<img src="https://static.igem.org/mediawiki/2015/b/b7/KU_Leuven_Motility2.jpg" width="92.4%">
+
−
</div>
+
−
<div class="whitespace">
+
−
</div>
+
  
−
<div class="subtext">
+
<br>
−
<!------
+
<br>
−
<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">---->
+
<br>
−
<p>
+
<br>
−
More coming soon
+
 
</p>
 
</p>
−
<!--------<p>The results of our experiments will appear here.
 
−
<br/>
 
−
<div class="more"><p>
 
−
Read more</p>
 
−
</div>
 
−
</p>--------->
 
−
</a>
 
−
</div>
 
 
</div>
 
</div>
 
</div>
 
</div>
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 +
 +
</br>
 +
 +
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Revision as of 07:57, 8 September 2015

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Interlab study

Results

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Table 1 Fluorescence measurements


D1, D2, D3 Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively


LB + Cam LB+Cam+Cells D1 D2 D3
512 601 737 685 751 9425 9322 9737 22786 25895 25048
546 594 754 641 641 9669 9545 9876 23159 26460 24785
549 587 722 660 697 9407 9321 9677 22719 25931 24935

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Table 2 Absorbance measurements


D1, D2, D3 Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively


LB + Cam LB+Cam+Cells D1 D2 D3
0.0434 0.2052 0.2174 0.1942 0.2201 0.2088 0.2082 0.2154 0.2139 0.2210 0.2218
0.0405 0.2000 0.2182 0.1974 0.1980 0.2108 0.2111 0.2123 0.2143 0.2179 0.2056
0.0379 0.2064 0.2141 0.1961 0.2036 0.2137 0.2117 0.2164 0.2202 0.2261 0.2083

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Table 3 Values for Biological replicates


D1, D2, D3 Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively

B1, B2, B3 Biological replicates of the respective devices

T1, T2, T3 Technical replicates


D1 D2 D3 LB+Cam+Cells
T1 T2 T3 T1 T2 T3 T1 T2 T2
B1 3390 3456 3372 45139 45868 44020 106526 108068 103174 2929
B2 3527 3247 3366 44774 45216 44029 117172 121432 114688 2970
B3 3399 3237 3426 45204 46519 44718 112931 120550 119707 2844
Brep Avg 3439 3313 3388 45039 45868 44256 112210 116683 112523
BRep SD 270 101 162 189 532 327 4376 6102 6921

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Table 4 Values for Technical replicates


D1, D2, D3 Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively

B1, B2, B3 Biological replicates of the respective devices

T1, T2, T3 Technical replicates


D1 D2 D3 LB+Cam+ Cells
B1 B2 B3 B1 B2 B3 B1 B2 B3
T1 3390 3527 3399 45139 44774 45204 106526 117172 112931 2929
T2 3456 3247 3237 45868 45216 46519 108068 121432 120550 2970
T3 3372 3366 3426 44020 44029 44718 103174 114688 119707 2844
TRep Avg 3406 3380 3354 45009 44673 45480 105923 117764 117729 2914
TRep SD 36 115 140 760 490 761 2043 2785 3410 52

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Table 5 Standard graph


Fluorescein standard
concentration (ng/mL) F1 F2 F3 average F average F' SD
0 9 13 12 11 0 1.699673171
10 1140 1168 1151 1153 1142 11.5181017
25 2543 2600 2631 2591 2580 36.4447832
50 4822 5097 5154 5024 5013 144.9513328
125 12879 12902 12931 12904 12893 21.27596453
250 25494 25761 25765 25673 25662 126.8183303
375 37474 37505 37899 37626 37615 193.4545597
500 46698 49051 48798 48182 48171 1054.652023

Figure 1
Standard curve for fluorescein

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Table 6 Extrapolation of GFP concentration using fluorescence measurements and fluorescein standard


D1 D2 D3
Brep Avg 3439 3313 3388 45039 45868 44256 112210 116683 112523
(Brep Avg) - (LB+cam+cells avg) 525 399 474 42125 42954 41342 109296 113769 109609
Concentration (ng/mL) 1.929 0.639 1.407 427.88 436.36 419.88 1115.64 1161.44 1118.85
TRep Avg 3406 3380 3354 45009 44673 45480 105923 117764 117729
(Trep avg) - (LB+cam+cellsavg) 492 466 440 42095 41759 42566 103009 114850 114815
Concentration (ng/mL) 1.591 1.325 1.059 427.56 424.12 432.39 1051.27 1172.51 1172.15

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