Difference between revisions of "Team:York/Experiments"

 
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<h2>Experiments &amp; Protocols</h2>
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<h2>Notebook</h2>
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<p>The following is a weekly description of the experiments carried out each week. To see the protocols click <a href="https://2015.igem.org/Team:York/Protocols">here</a></p>
  
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<p>Describe the experiments, research and protocols you used in your iGEM project.</p>
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<h3>Dry Lab Work Period</h3>
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<ul>
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<li>28/01: Brief introductory meeting about the iGEM competition
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<li>17/03: The official iGEM team to represent the University of York was formed!
 +
<li>05/05: Article published in Nouse (University newspaper) about the 2015 iGEM team.
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<li>03/06: We were invited to Science and Technology Alumni Network discussion event (outreach)
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<li>04/06: Article published on the University's central news about this years iGEM team.
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<li>12/06: A meeting was held with Yorkshire Water to discuss water remediation processes in the Yorkshire region
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<li>13/06: We were invited to a University of York Biology Alumni event
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<li>15/06:
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<ol>
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<li>Some of us went to a round-table discussion about the impact of Santander at York as some of the team members won the International Connections Award by Santander.</li>
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<li>Article published on Mind the Horizon about University of York iGEM</li></ol></li>
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<li>19/06:
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  <ol>
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<li> Lab safety induction was carried out</li>
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<li>We passed out pamphlets at the York Festival of Ideas to raise awareness about the iGEM competition and synthetic biology.</li></ol></li>
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<li> 26&27/06: We participated in giving talks to prospective students about iGEM.</li>
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</ul>  
  
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<h5>What should this page contain?</h5>
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<h3>Week 1 - June 23-27 </h3>
 
<ul>
 
<ul>
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<li> Protocols </li> <a href="https://2015.igem.org/Team:York/Protocols">Protocols page</a>
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<li>24/07: Wet lab practice day- reviewed basic lab protocols- making LB, agar plates, competent cells, transformations, PCR </li>
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<li> Experiments </li>
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<li>Documentation of the development of your project </li>
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</ul>
 
</ul>
  
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<h3>Week 2 - June 29- July 3 </h3>
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<ul>
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<li>01/07:Competent cells made</li>
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<li>02/07:Plates were streaked with two isolates of each deletion wanted from the Keio collection [PPX, PPK, PstA, PstB, PstC, PstS and PhoE] These have been collected and put in the incubating (37°C) room.</li>
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<li>03/07:Competent cells were tested with iGEM transformation efficiency kit</li>
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</ul>
  
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<h3>Week 3 - July 6-10 </h3>
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<ul>
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<li>07/07: More agar plates made, next set of competent cell procedure started</li>
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<li>07/07: YUfund page done and submitted</li>
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</ul>
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 +
<h3>Week 4 - July 13-17  </h3>
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<ul>
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<li>14/07: Vistited Badger Hill Primary School and held a bacteria workshop for the students</li>
 +
<li>15/07: first day of demonstrations of cell transformations and aseptic technique to sixth formers</li>
 +
<li>16/07: second day of demonstrations to sixth formers:analysing transformations and building bioreactors</li>
 +
<Li>17/07: analysing bioreactors with sixth formers</li>
 +
<li>17/07: first attempt at phosphate assay</li>
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</ul>
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 +
<h3>Week 5 - July 20-24  </h3>
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<ul>
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<li>20/07:Phosphate assay was started for KEIO collection</li>
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<li>20/07:Colony PCR on SmPstSCAB, EcPstSCAB and EcPhoE</li>
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<li>20/07:Next set of competent cells made </li>
 +
<li>23/07:Visit to AgeUK LunchClub to explain about the GM</li>
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</ul>
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<h3>Week 6 - July 27-31  </h3>
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<ul>
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<li>28/07:First day of outreaching event to Saint Helen's CHurch, York, to carry out strawberry extraction practical and briefly explain about the steps involved for GM </li>
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<li>29/07:Second (and the last day) of outreaching event to Saint Helen's Church, York, to carry out strawberry extraction practical and briefly explain about the steps involved for GM </li>
 +
<li>30/07:After several repeats the phosphate assay team managed to get significant data</li>
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</ul>
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 +
<h3>Week 7 - August 3-7  </h3>
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<ul>
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<li>03/08:Plasmid (pBC 29 Keasling collection )loss experiment initiated</li>
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</ul>
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<h3>Week 8 - August 10-14    </h3>
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<ul>
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</ul>
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<h3>Week 9 - August 17-21  </h3>
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<ul>
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</ul>
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<h3>Week 10 - August 24-28    </h3>
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<ul>
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</ul>
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<h3>Week 11 - August 31- September 4    </h3>
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<ul>
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</ul>
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<h3>Week 12 - September 7-11  </h3>
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<ul>
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</ul>
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<h3>Week 13 - September 13-18  </h3>
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<ul>
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</ul>
  
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<h4>Inspiration</h4>
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<!-- <h4>Inspiration</h4>  
 
<ul>
 
<ul>
 
<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
 
<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
 
<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
 
</ul>
 
</ul>
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</div>
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Latest revision as of 09:59, 8 September 2015

Notebook

The following is a weekly description of the experiments carried out each week. To see the protocols click here

Dry Lab Work Period

  • 28/01: Brief introductory meeting about the iGEM competition
  • 17/03: The official iGEM team to represent the University of York was formed!
  • 05/05: Article published in Nouse (University newspaper) about the 2015 iGEM team.
  • 03/06: We were invited to Science and Technology Alumni Network discussion event (outreach)
  • 04/06: Article published on the University's central news about this years iGEM team.
  • 12/06: A meeting was held with Yorkshire Water to discuss water remediation processes in the Yorkshire region
  • 13/06: We were invited to a University of York Biology Alumni event
  • 15/06:
    1. Some of us went to a round-table discussion about the impact of Santander at York as some of the team members won the International Connections Award by Santander.
    2. Article published on Mind the Horizon about University of York iGEM
  • 19/06:
    1. Lab safety induction was carried out
    2. We passed out pamphlets at the York Festival of Ideas to raise awareness about the iGEM competition and synthetic biology.
  • 26&27/06: We participated in giving talks to prospective students about iGEM.

Week 1 - June 23-27

  • 24/07: Wet lab practice day- reviewed basic lab protocols- making LB, agar plates, competent cells, transformations, PCR

Week 2 - June 29- July 3

  • 01/07:Competent cells made
  • 02/07:Plates were streaked with two isolates of each deletion wanted from the Keio collection [PPX, PPK, PstA, PstB, PstC, PstS and PhoE] These have been collected and put in the incubating (37°C) room.
  • 03/07:Competent cells were tested with iGEM transformation efficiency kit

Week 3 - July 6-10

  • 07/07: More agar plates made, next set of competent cell procedure started
  • 07/07: YUfund page done and submitted

Week 4 - July 13-17

  • 14/07: Vistited Badger Hill Primary School and held a bacteria workshop for the students
  • 15/07: first day of demonstrations of cell transformations and aseptic technique to sixth formers
  • 16/07: second day of demonstrations to sixth formers:analysing transformations and building bioreactors
  • 17/07: analysing bioreactors with sixth formers
  • 17/07: first attempt at phosphate assay

Week 5 - July 20-24

  • 20/07:Phosphate assay was started for KEIO collection
  • 20/07:Colony PCR on SmPstSCAB, EcPstSCAB and EcPhoE
  • 20/07:Next set of competent cells made
  • 23/07:Visit to AgeUK LunchClub to explain about the GM

Week 6 - July 27-31

  • 28/07:First day of outreaching event to Saint Helen's CHurch, York, to carry out strawberry extraction practical and briefly explain about the steps involved for GM
  • 29/07:Second (and the last day) of outreaching event to Saint Helen's Church, York, to carry out strawberry extraction practical and briefly explain about the steps involved for GM
  • 30/07:After several repeats the phosphate assay team managed to get significant data

Week 7 - August 3-7

  • 03/08:Plasmid (pBC 29 Keasling collection )loss experiment initiated

Week 8 - August 10-14

Week 9 - August 17-21

Week 10 - August 24-28

Week 11 - August 31- September 4

Week 12 - September 7-11

Week 13 - September 13-18