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Revision as of 11:42, 8 September 2015

Innovative Autonomous
Kill Switch for Bacteria

That's What We Do.

We Are The Technion HS 2015 iGEM Team

Welcome to our wiki.

Scroll to see What, Why, How and Who

What we do

We design

a chain of reactions which ends with in a poison

We implement

it using genetic engineering on a plasmid

We introduce

it into the bacteria in question

We control

the bacteria's lifespan by adjusting the amount of 'food' we give

We rest because the kill switch will eliminate the bacteria at the right time

Why should you care

Genetically modified organisms (GMO)

are everywhere

food, medicines, industry...

GMOs are scary

52% of the population think Genetically Modified foods are unsafe

Uncontrolled GMO growth might harm

the environment
Our system

helps to solve these problems by controlling GMO's population

and enables GMO-based industry to control precisely

how much product

will be produced

How we do it

Our biobrick

is composed out of three parts

The first and second parts

are responsible for controlling expression in the third part- the killing part.

we achieve

control

over the first two parts by using AHL (N-Acyl homoserine lactones) as an inducer- the 'food'.

The AHL

binds with LuxR creating a complex that inhibits expression of some genes while AiiA degrades the AHL. that why when the 'food' runs out the poison in the third part is expressed and the cell dies.

Who we are

Genetically modified organisms (GMO)

are everywhere

food, medicines, industry...

GMOs are scary

52% of the population think Genetically Modified foods are unsafe

Uncontrolled GMO growth might harm

the environment
Our system

helps to solve these problems by controlling GMO's population

and enables GMO-based industry to control precisely

how much product

will be produced

Scroll for more