Difference between revisions of "Team:TU Dresden/Notebook/LabJournal"

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<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 1: (20-26 July)</div>
 
<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 1: (20-26 July)</div>
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  <li style="margin-bottom: 10px;line-height:1.8;">Plasmids were received (T18, ZHER2, LZ-T18, LZ-T25 and HER2 codon) and diluted. Also two pSB1C3 plasmids from iGEM containing RBS (<a style="text-decoration:none;" href="http://parts.igem.org/Part:BBa_B0030"><font color="#045FB4">BBa_E0020</font></a>) and CFP (<a style="text-decoration:none;" href="http://parts.igem.org/Part:BBa_E0020"><font color="#045FB4">BBa_E0020</font></a>) were diluted.</li>
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  <li style="margin-bottom: 10px;line-height:1.8;">Preparation of <i>E. coli</i> GB05 and set cultures overnight.</li>
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  <li style="margin-bottom: 10px;line-height:1.8;">Electroporation of the cells with the plasmids.</li>
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  <li style="margin-bottom: 10px;line-height:1.8;">Transfected cells were streaked out on chloramphenicol (Cm) and kanamycin (kan) plates.</li>
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  <li style="margin-bottom: 10px;line-height:1.8;">Colonies were picked and mixed with Cm and kan. These were stored in the fridge.</li>
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  <li style="margin-bottom: 10px;line-height:1.8;">Streaking out colonies from DHM1, BTH101 and K12 plates.</li>
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</ul>
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<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 2 (27 July-2 August)</div>
 
<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 2 (27 July-2 August)</div>
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  <li style="margin-bottom: 10px;line-height:1.8;">A miniprep was done with the seven cultures with the plasmids. A glycerol stock of each culture was saved.</li>
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  <li style="margin-bottom: 10px;line-height:1.8;">The concentration of the plasmids was measured using the nanodrop.</li>
 +
  <li style="margin-bottom: 10px;line-height:1.8;">A digestion of the plasmids was done and a gel was run.</li>
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</ul></div>
  
 
<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 3 (3-9 August)</div>
 
<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 3 (3-9 August)</div>
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<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 4 (10-16 August)</div>
 
<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 4 (10-16 August)</div>
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<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 5 ()</div>
 
<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 5 ()</div>
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<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 6 ()</div>
 
<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 6 ()</div>
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<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 7 ()</div>
 
<div class="technology" style="font-family: 'Open Sans', Arial, sans-serif;">Week 7 ()</div>
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Revision as of 21:42, 9 September 2015


Lab Journal

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Week 1: (20-26 July)
  • Plasmids were received (T18, ZHER2, LZ-T18, LZ-T25 and HER2 codon) and diluted. Also two pSB1C3 plasmids from iGEM containing RBS (BBa_E0020) and CFP (BBa_E0020) were diluted.
  • Preparation of E. coli GB05 and set cultures overnight.
  • Electroporation of the cells with the plasmids.
  • Transfected cells were streaked out on chloramphenicol (Cm) and kanamycin (kan) plates.
  • Colonies were picked and mixed with Cm and kan. These were stored in the fridge.
  • Streaking out colonies from DHM1, BTH101 and K12 plates.
Week 2 (27 July-2 August)
  • A miniprep was done with the seven cultures with the plasmids. A glycerol stock of each culture was saved.
  • The concentration of the plasmids was measured using the nanodrop.
  • A digestion of the plasmids was done and a gel was run.
Week 3 (3-9 August)
Week 4 (10-16 August)
Week 5 ()
Week 6 ()
Week 7 ()